CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-002143
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
 CAA26306.1; AAA59987.1; AAA59987.1; AAA59987.1; AAA59987.1; AAA59987.1; AAA59987.1; AAA59987.1; AAA59987.1; AAA59987.1; AAA59987.1; AAA59989.1; AAA61211.1; AAA61212.1; AAA59988.1; AAA59988.1; AAA59988.1; AAA59988.1; AAA59988.1; AAA59988.1; AAA59988.1; AAA59988.1; AAA59988.1; AAA59988.1; CAA25652.1; CAA42626.1; CAA42627.1; CAA42628.1; CAA42629.1; CAA42630.1; CAA42631.1; CAA42632.1; CAA42633.1; CAA42634.1; CAA42635.1; AAG28785.1; ABA29753.1; ABA29754.1; ABA29755.1; ABA29756.1; ABA29757.1; ABB80262.1; ABB80266.1; CAA38095.1; AAC12971.1; AAV80424.1; AAD28535.1; AAD28535.1; AAD28628.1; AAD28628.1; BAC16799.1; BAG35463.1; EAW90143.1; EAW90144.1; AAH03596.1; AAR10356.1; AAQ90158.1; AAR13239.1; AAB39322.1; AAF36362.1; AAF36354.1; AAF36355.1; AAF36356.1; AAF36357.1; AAF36358.1; AAF36359.1; AAF36360.1; AAF36361.1; AAF36374.1; AAF36375.1; AAF36376.1; AAF36377.1; AAF36378.1; AAF36379.1; AAF36380.1; AAF36381.1; AAF36382.1; AAF63442.1; AAF63442.1; AAF63443.1; AAK76358.1; AAK76359.1; AAP30003.1 
Protein Name
 Cellular tumor antigen p53 
Protein Synonyms/Alias
 Antigen NY-CO-13; Phosphoprotein p53; Tumor suppressor p53 
Gene Name
 TP53 
Gene Synonyms/Alias
 P53 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
318LKDAQAGKEPGGSRAubiquitination[1]
347RHKKLMFKTEGPDSDubiquitination[1]
Reference
 [1] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661] 
Functional Description
 Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression. In cooperation with mitochondrial PPIF is involved in activating oxidative stress-induced necrosis; the function is largely independent of transcription. Induces the transcription of long intergenic non-coding RNA p21 (lincRNA-p21) and lincRNA- Mkln1. LincRNA-p21 participates in TP53-dependent transcriptional repression leading to apoptosis and seem to have to effect on cell-cycle regulation. Implicated in Notch signaling cross-over. Prevents CDK7 kinase activity when associated to CAK complex in response to DNA damage, thus stopping cell cycle progression. Isoform 2 enhances the transactivation activity of isoform 1 from some but not all TP53-inducible promoters. Isoform 4 suppresses transactivation activity and impairs growth suppression mediated by isoform 1. Isoform 7 inhibits isoform 1-mediated apoptosis. 
Sequence Annotation
 DNA_BIND 102 292
 REGION 1 83 Interaction with HRMT1L2.
 REGION 1 44 Transcription activation (acidic).
 REGION 66 110 Interaction with WWOX.
 REGION 100 370 Interaction with HIPK1 (By similarity).
 REGION 100 300 Required for interaction with ZNF385A.
 REGION 113 236 Required for interaction with FBXO42.
 REGION 116 292 Interaction with AXIN1 (By similarity).
 REGION 241 248 Interacts with the 53BP2 SH3 domain.
 REGION 256 294 Interaction with E4F1.
 REGION 273 280 Interaction with DNA.
 REGION 300 393 Interaction with CARM1.
 REGION 319 360 Interaction with HIPK2.
 REGION 325 356 Oligomerization.
 REGION 359 363 Interaction with USP7.
 REGION 368 387 Basic (repression of DNA-binding).
 MOTIF 17 25 TADI.
 MOTIF 48 56 TADII.
 MOTIF 305 321 Bipartite nuclear localization signal.
 MOTIF 339 350 Nuclear export signal.
 MOTIF 370 372 [KR]-[STA]-K motif.
 METAL 176 176 Zinc.
 METAL 179 179 Zinc.
 METAL 238 238 Zinc.
 METAL 242 242 Zinc.
 MOD_RES 9 9 Phosphoserine; by HIPK4.
 MOD_RES 15 15 Phosphoserine; by CDK5, PRPK, AMPK, NUAK1
 MOD_RES 18 18 Phosphothreonine; by CK1, VRK1 and VRK2.
 MOD_RES 20 20 Phosphoserine; by CHEK2, CK1 and PLK3.
 MOD_RES 33 33 Phosphoserine; by CDK5 and CDK7.
 MOD_RES 37 37 Phosphoserine; by MAPKAPK5.
 MOD_RES 46 46 Phosphoserine; by CDK5, DYRK2, HIPK2 and
 MOD_RES 55 55 Phosphothreonine; by TAF1 and GRK5.
 MOD_RES 120 120 N6-acetyllysine; by KAT6A.
 MOD_RES 183 183 Phosphoserine; by AURKB.
 MOD_RES 269 269 Phosphoserine; by AURKB.
 MOD_RES 284 284 Phosphothreonine; by AURKB.
 MOD_RES 305 305 N6-acetyllysine.
 MOD_RES 315 315 Phosphoserine; by AURKA, CDK1 and CDK2.
 MOD_RES 370 370 N6,N6-dimethyllysine; alternate.
 MOD_RES 370 370 N6-methyllysine; by SMYD2; alternate.
 MOD_RES 372 372 N6-methyllysine; by SETD7.
 MOD_RES 373 373 N6,N6-dimethyllysine; by EHMT1 and EHMT2;
 MOD_RES 373 373 N6-acetyllysine; alternate.
 MOD_RES 381 381 N6-acetyllysine.
 MOD_RES 382 382 N6,N6-dimethyllysine; alternate.
 MOD_RES 382 382 N6-acetyllysine; by KAT6A; alternate.
 MOD_RES 382 382 N6-methyllysine; by SETD8; alternate.
 MOD_RES 392 392 Phosphoserine; by CK2, CDK2 and NUAK1.
 CROSSLNK 291 291 Glycyl lysine isopeptide (Lys-Gly)
 CROSSLNK 292 292 Glycyl lysine isopeptide (Lys-Gly)
 CROSSLNK 386 386 Glycyl lysine isopeptide (Lys-Gly)  
Keyword
 3D-structure; Acetylation; Activator; Alternative promoter usage; Alternative splicing; Apoptosis; Cell cycle; Complete proteome; Cytoplasm; Disease mutation; DNA-binding; Endoplasmic reticulum; Glycoprotein; Host-virus interaction; Isopeptide bond; Li-Fraumeni syndrome; Metal-binding; Methylation; Mitochondrion; Necrosis; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Transcription; Transcription regulation; Tumor suppressor; Ubl conjugation; Zinc. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 393 AA 
Protein Sequence
MDDLMLSPDD IEQWFTEDPG PDEAPRMPEA APPVAPAPAA PTPAAPAPAP SWPLSSSVPS 60
QKTYQGSYGF RLGFLHSGTA KSVTCTYSPA LNKMFCQLAK TCPVQLWVDS TPPPGTRVRA 120
MAIYKQSQHM TEVVRRCPHH ERCSDSDGLA PPQHLIRVEG NLRVEYLDDR NTFRHSVVVP 180
YEPPEVGSDC TTIHYNYMCN SSCMGGMNRR PILTIITLED SSGNLLGRNS FEVRVCACPG 240
RDRRTEEENL RKKGEPHHEL PPGSTKRALP NNTSSSPQPK KKPLDGEYFT LQIRGRERFE 300
MFRELNEALE LKDAQAGKEP GGSRAHSSHL KSKKGQSTSR HKKLMFKTEG PDSD 354 
Gene Ontology
 GO:0005737; C:cytoplasm; IDA:UniProtKB.
 GO:0005829; C:cytosol; IDA:UniProtKB.
 GO:0005783; C:endoplasmic reticulum; IEA:UniProtKB-SubCell.
 GO:0005759; C:mitochondrial matrix; IEA:UniProtKB-SubCell.
 GO:0005739; C:mitochondrion; IDA:UniProtKB.
 GO:0000790; C:nuclear chromatin; IDA:BHF-UCL.
 GO:0016363; C:nuclear matrix; IDA:UniProtKB.
 GO:0005730; C:nucleolus; IDA:UniProtKB.
 GO:0005634; C:nucleus; IDA:UniProtKB.
 GO:0016605; C:PML body; IDA:UniProtKB.
 GO:0043234; C:protein complex; IDA:BHF-UCL.
 GO:0005657; C:replication fork; IBA:RefGenome.
 GO:0005524; F:ATP binding; IDA:UniProtKB.
 GO:0003682; F:chromatin binding; IDA:UniProtKB.
 GO:0005507; F:copper ion binding; IDA:UniProtKB.
 GO:0003684; F:damaged DNA binding; IBA:RefGenome.
 GO:0035033; F:histone deacetylase regulator activity; IEA:Compara.
 GO:0002039; F:p53 binding; IBA:RefGenome.
 GO:0001077; F:RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription; IEA:Compara.
 GO:0001228; F:RNA polymerase II transcription regulatory region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription; IDA:BHF-UCL.
 GO:0044212; F:transcription regulatory region DNA binding; IDA:BHF-UCL.
 GO:0008270; F:zinc ion binding; TAS:UniProtKB.
 GO:0002326; P:B cell lineage commitment; IEA:Compara.
 GO:0006284; P:base-excision repair; TAS:UniProtKB.
 GO:0007596; P:blood coagulation; TAS:Reactome.
 GO:0007050; P:cell cycle arrest; IMP:UniProtKB.
 GO:0030154; P:cell differentiation; TAS:UniProtKB.
 GO:0008283; P:cell proliferation; TAS:UniProtKB.
 GO:0034613; P:cellular protein localization; IDA:UniProtKB.
 GO:0035690; P:cellular response to drug; IEP:UniProtKB.
 GO:0042149; P:cellular response to glucose starvation; IDA:UniProtKB.
 GO:0071456; P:cellular response to hypoxia; IEP:UniProtKB.
 GO:0071479; P:cellular response to ionizing radiation; IMP:BHF-UCL.
 GO:0034644; P:cellular response to UV; IBA:RefGenome.
 GO:0007417; P:central nervous system development; IEA:Compara.
 GO:0051276; P:chromosome organization; IEA:Compara.
 GO:0008340; P:determination of adult lifespan; ISS:BHF-UCL.
 GO:0006977; P:DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; TAS:Reactome.
 GO:0006978; P:DNA damage response, signal transduction by p53 class mediator resulting in transcription of p21 class mediator; IMP:BHF-UCL.
 GO:0000733; P:DNA strand renaturation; IDA:UniProtKB.
 GO:0006302; P:double-strand break repair; IEA:Compara.
 GO:0006983; P:ER overload response; IDA:MGI.
 GO:0007369; P:gastrulation; IEA:Compara.
 GO:0001701; P:in utero embryonic development; IEA:Compara.
 GO:0006917; P:induction of apoptosis; IEA:Compara.
 GO:0042771; P:intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator; IDA:UniProtKB.
 GO:0071850; P:mitotic cell cycle arrest; IEA:Compara.
 GO:0035264; P:multicellular organism growth; IEA:Compara.
 GO:0043066; P:negative regulation of apoptotic process; IDA:UniProtKB.
 GO:0030308; P:negative regulation of cell growth; IMP:UniProtKB.
 GO:0008156; P:negative regulation of DNA replication; IEA:Compara.
 GO:0048147; P:negative regulation of fibroblast proliferation; IMP:UniProtKB.
 GO:0051097; P:negative regulation of helicase activity; TAS:UniProtKB.
 GO:0007406; P:negative regulation of neuroblast proliferation; IEA:Compara.
 GO:0000122; P:negative regulation of transcription from RNA polymerase II promoter; IDA:UniProtKB.
 GO:0030512; P:negative regulation of transforming growth factor beta receptor signaling pathway; IEA:Compara.
 GO:0051402; P:neuron apoptotic process; IEA:Compara.
 GO:0006289; P:nucleotide-excision repair; IMP:UniProtKB.
 GO:0097252; P:oligodendrocyte apoptotic process; IDA:UniProtKB.
 GO:0090403; P:oxidative stress-induced premature senescence; IMP:BHF-UCL.
 GO:0090343; P:positive regulation of cell aging; IEA:Compara.
 GO:0031065; P:positive regulation of histone deacetylation; IBA:RefGenome.
 GO:2001244; P:positive regulation of intrinsic apoptotic signaling pathway; IMP:UniProtKB.
 GO:0043525; P:positive regulation of neuron apoptotic process; IBA:RefGenome.
 GO:0050731; P:positive regulation of peptidyl-tyrosine phosphorylation; ISS:BHF-UCL.
 GO:0032461; P:positive regulation of protein oligomerization; IDA:UniProtKB.
 GO:2000379; P:positive regulation of reactive oxygen species metabolic process; IMP:BHF-UCL.
 GO:0090200; P:positive regulation of release of cytochrome c from mitochondria; IDA:UniProtKB.
 GO:0070245; P:positive regulation of thymocyte apoptotic process; ISS:BHF-UCL.
 GO:0045944; P:positive regulation of transcription from RNA polymerase II promoter; IDA:UniProtKB.
 GO:0000060; P:protein import into nucleus, translocation; IEA:Compara.
 GO:0008104; P:protein localization; IDA:UniProtKB.
 GO:0051262; P:protein tetramerization; TAS:UniProtKB.
 GO:0007265; P:Ras protein signal transduction; IEP:BHF-UCL.
 GO:0046902; P:regulation of mitochondrial membrane permeability; TAS:UniProtKB.
 GO:0001836; P:release of cytochrome c from mitochondria; IEA:Compara.
 GO:0090399; P:replicative senescence; IMP:BHF-UCL.
 GO:0046677; P:response to antibiotic; IEP:UniProtKB.
 GO:0010332; P:response to gamma radiation; IMP:BHF-UCL.
 GO:0009651; P:response to salt stress; IEA:Compara.
 GO:0010165; P:response to X-ray; IBA:RefGenome.
 GO:0009303; P:rRNA transcription; IEA:Compara.
 GO:0001756; P:somitogenesis; IEA:Compara.
 GO:0033077; P:T cell differentiation in thymus; IEA:Compara.
 GO:0002360; P:T cell lineage commitment; IEA:Compara.
 GO:0002309; P:T cell proliferation involved in immune response; IEA:Compara.
 GO:0007179; P:transforming growth factor beta receptor signaling pathway; IEA:Compara.
 GO:0019048; P:virus-host interaction; IEA:UniProtKB-KW. 
Interpro
 IPR008967; p53-like_TF_DNA-bd.
 IPR012346; p53/RUNT-type_TF_DNA-bd.
 IPR011615; p53_DNA-bd.
 IPR010991; p53_tetrameristn.
 IPR013872; p53_transactivation_domain.
 IPR002117; p53_tumour_suppressor. 
Pfam
 PF00870; P53
 PF08563; P53_TAD
 PF07710; P53_tetramer 
SMART
  
PROSITE
 PS00348; P53 
PRINTS
 PR00386; P53SUPPRESSR.