CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-014825
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 SUMO-activating enzyme subunit 1 
Protein Synonyms/Alias
 Ubiquitin-like 1-activating enzyme E1A; SUMO-activating enzyme subunit 1, N-terminally processed 
Gene Name
 Sae1 
Gene Synonyms/Alias
 Uble1a 
Created Date
 July 27, 2013 
Organism
 Rattus norvegicus (Rat) 
NCBI Taxa ID
 10116 
Lysine Modification
Position
Peptide
Type
References
4****MVEKEEVSGGGacetylation[1]
35LWGLEAQKRLRASRVacetylation[1]
198VEDGPDAKRAKLDSSacetylation[1]
201GPDAKRAKLDSSETTacetylation[1]
Reference
 [1] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
 Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
 Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405] 
Functional Description
 The heterodimer acts as a E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2 (By similarity). 
Sequence Annotation
 MOD_RES 1 1 N-acetylmethionine (By similarity).
 MOD_RES 2 2 N-acetylvaline; in SUMO-activating enzyme  
Keyword
 Acetylation; Complete proteome; Ligase; Nucleus; Reference proteome; Ubl conjugation pathway. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 349 AA 
Protein Sequence
MVEKEEVSGG GGGISEEEAA QYDRQIRLWG LEAQKRLRAS RVLIVGMKGL GAEIAKNLIL 60
AGVKGLTMLD HEQVSPEDLG AQFLIRTGSV GQNRAEASLE RAQNLNPMVD VKVDTEDIEK 120
KPESFFTEFD AVCLTCCSKD VIIKVDQICH RNSIKFFTGD VFGYHGYTFA NLGEHEFVEE 180
KTKVTKVSQG VEDGPDAKRA KLDSSETTMV KKKVLFCPVK EALAVDWSGE KAQAALKRTA 240
PDYFLLQVLL KFRTDKGRDP TSDSYSEDAE LLLQIRNDVF DSLGVSPDLL PDDFVRYCFS 300
EMAPVCAVVG GILAQEIVKA LSQRDPPHNN FFFFDGMKGS GIVECLGPQ 349 
Gene Ontology
 GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
 GO:0016874; F:ligase activity; IEA:UniProtKB-KW.
 GO:0008022; F:protein C-terminus binding; ISS:UniProtKB.
 GO:0019948; F:SUMO activating enzyme activity; IEA:Compara.
 GO:0016925; P:protein sumoylation; ISS:UniProtKB. 
Interpro
 IPR009036; Molybdenum_cofac_synth_MoeB.
 IPR016040; NAD(P)-bd_dom.
 IPR000594; ThiF_NAD_FAD-bd.
 IPR000011; UBQ/SUMO-activ_enz_E1-like. 
Pfam
 PF00899; ThiF 
SMART
  
PROSITE
  
PRINTS
 PR01849; UBIQUITINACT.