CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-000367
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Phosphoribosylformylglycinamidine synthase 
Protein Synonyms/Alias
 FGAM synthase; FGAMS; Formylglycinamide ribotide amidotransferase; FGARAT; Formylglycinamide ribotide synthetase 
Gene Name
 PFAS 
Gene Synonyms/Alias
 KIAA0361 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
59PSAEETKKLMWLFGCubiquitination[1]
186EGRLALEKANQELGLubiquitination[1, 2, 3]
205WDLDFYTKRFQELQRubiquitination[1, 2, 4]
236HSRHWFFKGQLHVDGubiquitination[1, 5]
268SNPNNVLKFCDNSSAubiquitination[1, 2]
279NSSAIQGKEVRFLRPubiquitination[1, 2, 3, 5, 6, 7]
422GQRREWIKPIMFSGGubiquitination[1, 2, 4, 7]
440MEADHISKEAPEPGMubiquitination[1]
451EPGMEVVKVGGPVYRubiquitination[1, 4]
494GDPEMEQKMNRVIRAubiquitination[1, 4]
507RACVEAPKGNPICSLubiquitination[1]
527GGNGNVLKELSDPAGubiquitination[1]
639VLGKMPRKEFFLQRKubiquitination[1]
646KEFFLQRKPPMLQPLubiquitination[1, 5]
677RLPAVASKRYLTNKVubiquitination[1]
683SKRYLTNKVDRSVGGubiquitination[1, 4, 5]
737VKSLLDPKVAARLAVubiquitination[1, 4]
843ATVTPDLKHPEGRGHubiquitination[1]
900SITQGLLKDRLLCSGubiquitination[1, 4, 5, 8]
1051CLPPTFPKASVPREPubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [3] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
 Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
 J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572]
 [4] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [5] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [6] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
 Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
 J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931]
 [7] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [8] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473
Functional Description
  
Sequence Annotation
 DOMAIN 1064 1302 Glutamine amidotransferase type-1.
 NP_BIND 322 333 ATP (Potential).
 ACT_SITE 1158 1158 For GATase activity (By similarity).
 MOD_RES 569 569 Phosphoserine.
 MOD_RES 619 619 Phosphothreonine.
 MOD_RES 623 623 Phosphothreonine.  
Keyword
 ATP-binding; Complete proteome; Cytoplasm; Glutamine amidotransferase; Ligase; Nucleotide-binding; Phosphoprotein; Polymorphism; Purine biosynthesis; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1338 AA 
Protein Sequence
MSPVLHFYVR PSGHEGAAPG HTRRKLQGKL PELQGVETEL CYNVNWTAEA LPSAEETKKL 60
MWLFGCPLLL DDVARESWLL PGSNDLLLEV GPRLNFSTPT STNIVSVCRA TGLGPVDRVE 120
TTRRYRLSFA HPPSAEVEAI ALATLHDRMT EQHFPHPIQS FSPESMPEPL NGPINILGEG 180
RLALEKANQE LGLALDSWDL DFYTKRFQEL QRNPSTVEAF DLAQSNSEHS RHWFFKGQLH 240
VDGQKLVHSL FESIMSTQES SNPNNVLKFC DNSSAIQGKE VRFLRPEDPT RPSRFQQQQG 300
LRHVVFTAET HNFPTGVCPF SGATTGTGGR IRDVQCTGRG AHVVAGTAGY CFGNLHIPGY 360
NLPWEDPSFQ YPGNFARPLE VAIEASNGAS DYGNKFGEPV LAGFARSLGL QLPDGQRREW 420
IKPIMFSGGI GSMEADHISK EAPEPGMEVV KVGGPVYRIG VGGGAASSVQ VQGDNTSDLD 480
FGAVQRGDPE MEQKMNRVIR ACVEAPKGNP ICSLHDQGAG GNGNVLKELS DPAGAIIYTS 540
RFQLGDPTLN ALEIWGAEYQ ESNALLLRSP NRDFLTHVSA RERCPACFVG TITGDRRIVL 600
VDDRECPVRR NGQGDAPPTP LPTPVDLELE WVLGKMPRKE FFLQRKPPML QPLALPPGLS 660
VHQALERVLR LPAVASKRYL TNKVDRSVGG LVAQQQCVGP LQTPLADVAV VALSHEELIG 720
AATALGEQPV KSLLDPKVAA RLAVAEALTN LVFALVTDLR DVKCSGNWMW AAKLPGEGAA 780
LADACEAMVA VMAALGVAVD GGKDSLSMAA RVGTETVRAP GSLVISAYAV CPDITATVTP 840
DLKHPEGRGH LLYVALSPGQ HRLGGTALAQ CFSQLGEHPP DLDLPENLVR AFSITQGLLK 900
DRLLCSGHDV SDGGLVTCLL EMAFAGNCGL QVDVPVPRVD VLSVLFAEEP GLVLEVQEPD 960
LAQVLKRYRD AGLHCLELGH TGEAGPHAMV RVSVNGAVVL EEPVGELRAL WEETSFQLDR 1020
LQAEPRCVAE EERGLRERMG PSYCLPPTFP KASVPREPGG PSPRVAILRE EGSNGDREMA 1080
DAFHLAGFEV WDVTMQDLCS GAIGLDTFRG VAFVGGFSYA DVLGSAKGWA AAVTFHPRAG 1140
AELRRFRKRP DTFSLGVCNG CQLLALLGWV GGDPNEDAAE MGPDSQPARP GLLLRHNLSG 1200
RYESRWASVR VGPGPALMLR GMEGAVLPVW SAHGEGYVAF SSPELQAQIE ARGLAPLHWA 1260
DDDGNPTEQY PLNPNGSPGG VAGICSCDGR HLAVMPHPER AVRPWQWAWR PPPFDTLTTS 1320
PWLQLFINAR NWTLEGSC 1338 
Gene Ontology
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0004642; F:phosphoribosylformylglycinamidine synthase activity; IDA:UniProtKB.
 GO:0006189; P:'de novo' IMP biosynthetic process; NAS:UniProtKB.
 GO:0006541; P:glutamine metabolic process; IEA:UniProtKB-KW.
 GO:0006144; P:purine nucleobase metabolic process; TAS:Reactome.
 GO:0042493; P:response to drug; IEA:Compara. 
Interpro
 IPR010918; AIR_synth_C_dom.
 IPR000728; AIR_synth_N_dom.
 IPR017926; GATASE.
 IPR010073; PRibForGlyAmidine_synth.
 IPR016188; PurM_N-like. 
Pfam
 PF00586; AIRS
 PF02769; AIRS_C 
SMART
  
PROSITE
 PS51273; GATASE_TYPE_1 
PRINTS