CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-005017
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Cysteine--tRNA ligase 
Protein Synonyms/Alias
 Cysteinyl-tRNA synthetase; CysRS 
Gene Name
 cysS 
Gene Synonyms/Alias
 b0526; JW0515 
Created Date
 July 27, 2013 
Organism
 Escherichia coli (strain K12) 
NCBI Taxa ID
 83333 
Lysine Modification
Position
Peptide
Type
References
60YLRFLGYKLKYVRNIacetylation[1]
73NITDIDDKIIKRANEacetylation[1, 2]
76DIDDKIIKRANENGEacetylation[1]
98RMIAEMHKDFDALNIacetylation[1]
175RVDVVDDKRNPMDFVacetylation[1]
185PMDFVLWKMSKEGEPacetylation[1]
269VDREKMSKSLGNFFTacetylation[1]
282FTVRDVLKYYDAETVacetylation[1]
310NYSEENLKQARAALEacetylation[1]
328TALRGTDKTVAPAGGacetylation[1]
Reference
 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618]
 [2] The diversity of lysine-acetylated proteins in Escherichia coli.
 Yu BJ, Kim JA, Moon JH, Ryu SE, Pan JG.
 J Microbiol Biotechnol. 2008 Sep;18(9):1529-36. [PMID: 18852508] 
Functional Description
  
Sequence Annotation
 MOTIF 30 40 "HIGH" region.
 MOTIF 266 270 "KMSKS" region.
 METAL 28 28 Zinc.
 METAL 209 209 Zinc.
 METAL 234 234 Zinc.
 METAL 238 238 Zinc.
 BINDING 269 269 ATP (By similarity).  
Keyword
 3D-structure; Aminoacyl-tRNA synthetase; ATP-binding; Complete proteome; Cytoplasm; Direct protein sequencing; Ligase; Metal-binding; Nucleotide-binding; Protein biosynthesis; Reference proteome; Zinc. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 461 AA 
Protein Sequence
MLKIFNTLTR QKEEFKPIHA GEVGMYVCGI TVYDLCHIGH GRTFVAFDVV ARYLRFLGYK 60
LKYVRNITDI DDKIIKRANE NGESFVAMVD RMIAEMHKDF DALNILRPDM EPRATHHIAE 120
IIELTEQLIA KGHAYVADNG DVMFDVPTDP TYGVLSRQDL DQLQAGARVD VVDDKRNPMD 180
FVLWKMSKEG EPSWPSPWGA GRPGWHIECS AMNCKQLGNH FDIHGGGSDL MFPHHENEIA 240
QSTCAHDGQY VNYWMHSGMV MVDREKMSKS LGNFFTVRDV LKYYDAETVR YFLMSGHYRS 300
QLNYSEENLK QARAALERLY TALRGTDKTV APAGGEAFEA RFIEAMDDDF NTPEAYSVLF 360
DMAREVNRLK AEDMAAANAM ASHLRKLSAV LGLLEQEPEA FLQSGAQADD SEVAEIEALI 420
QQRLDARKAK DWAAADAARD RLNEMGIVLE DGPQGTTWRR K 461 
Gene Ontology
 GO:0005829; C:cytosol; IBA:RefGenome.
 GO:0005524; F:ATP binding; IPI:EcoliWiki.
 GO:0004817; F:cysteine-tRNA ligase activity; IDA:EcoliWiki.
 GO:0008270; F:zinc ion binding; IDA:EcoCyc.
 GO:0006423; P:cysteinyl-tRNA aminoacylation; IDA:EcoliWiki. 
Interpro
 IPR015803; Cys-tRNA-ligase.
 IPR015273; Cys-tRNA-synt_Ia_DALR.
 IPR024909; Cys-tRNA/MSH_ligase.
 IPR014729; Rossmann-like_a/b/a_fold.
 IPR009080; tRNAsynth_1a_anticodon-bd. 
Pfam
 PF09190; DALR_2
 PF01406; tRNA-synt_1e 
SMART
 SM00840; DALR_2 
PROSITE
 PS00178; AA_TRNA_LIGASE_I 
PRINTS
 PR00983; TRNASYNTHCYS.