CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-007478
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Hepatocyte nuclear factor 4-alpha 
Protein Synonyms/Alias
 HNF-4-alpha; Nuclear receptor subfamily 2 group A member 1; Transcription factor 14; TCF-14; Transcription factor HNF-4 
Gene Name
 HNF4A 
Gene Synonyms/Alias
 HNF4; NR2A1; TCF14 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
106SRQCVVDKDKRNQCRacetylation[1, 2]
108QCVVDKDKRNQCRYCacetylation[1]
126KCFRAGMKKEAVQNEacetylation[1]
127CFRAGMKKEAVQNERacetylation[1]
234HLLLGATKRSMVFKDubiquitination[3]
307KGLSDPGKIKRLRSQubiquitination[3]
458GAVATIVKPLSAIPQacetylation[4]
458GAVATIVKPLSAIPQubiquitination[3]
Reference
 [1] Acetylation regulates transcription factor activity at multiple levels.
 Soutoglou E, Katrakili N, Talianidis I.
 Mol Cell. 2000 Apr;5(4):745-51. [PMID: 10882110]
 [2] Regulation of cellular metabolism by protein lysine acetylation.
 Zhao S, Xu W, Jiang W, Yu W, Lin Y, Zhang T, Yao J, Zhou L, Zeng Y, Li H, Li Y, Shi J, An W, Hancock SM, He F, Qin L, Chin J, Yang P, Chen X, Lei Q, Xiong Y, Guan KL.
 Science. 2010 Feb 19;327(5968):1000-4. [PMID: 20167786]
 [3] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [4] Multiple post-translational modifications in hepatocyte nuclear factor 4α.
 Yokoyama A, Katsura S, Ito R, Hashiba W, Sekine H, Fujiki R, Kato S.
 Biochem Biophys Res Commun. 2011 Jul 15;410(4):749-53. [PMID: 21708125] 
Functional Description
 Transcriptionally controlled transcription factor. Binds to DNA sites required for the transcription of alpha 1- antitrypsin, apolipoprotein CIII, transthyretin genes and HNF1- alpha. May be essential for development of the liver, kidney and intestine. 
Sequence Annotation
 DNA_BIND 57 132 Nuclear receptor.
 ZN_FING 60 80 NR C4-type.
 ZN_FING 96 120 NR C4-type.
 MOD_RES 142 142 Phosphoserine.
 MOD_RES 166 166 Phosphothreonine.
 MOD_RES 167 167 Phosphoserine.
 MOD_RES 313 313 Phosphoserine; by AMPK.
 MOD_RES 429 429 Phosphothreonine (By similarity).
 MOD_RES 432 432 Phosphothreonine.
 MOD_RES 436 436 Phosphoserine.
 MOD_RES 458 458 N6-acetyllysine.
 CROSSLNK 234 234 Glycyl lysine isopeptide (Lys-Gly)
 CROSSLNK 307 307 Glycyl lysine isopeptide (Lys-Gly)  
Keyword
 3D-structure; Acetylation; Alternative promoter usage; Alternative splicing; Complete proteome; Diabetes mellitus; Disease mutation; DNA-binding; Isopeptide bond; Metal-binding; Nucleus; Phosphoprotein; Polymorphism; Receptor; Reference proteome; Transcription; Transcription regulation; Ubl conjugation; Zinc; Zinc-finger. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 474 AA 
Protein Sequence
MRLSKTLVDM DMADYSAALD PAYTTLEFEN VQVLTMGNDT SPSEGTNLNA PNSLGVSALC 60
AICGDRATGK HYGASSCDGC KGFFRRSVRK NHMYSCRFSR QCVVDKDKRN QCRYCRLKKC 120
FRAGMKKEAV QNERDRISTR RSSYEDSSLP SINALLQAEV LSRQITSPVS GINGDIRAKK 180
IASIADVCES MKEQLLVLVE WAKYIPAFCE LPLDDQVALL RAHAGEHLLL GATKRSMVFK 240
DVLLLGNDYI VPRHCPELAE MSRVSIRILD ELVLPFQELQ IDDNEYAYLK AIIFFDPDAK 300
GLSDPGKIKR LRSQVQVSLE DYINDRQYDS RGRFGELLLL LPTLQSITWQ MIEQIQFIKL 360
FGMAKIDNLL QEMLLGGSPS DAPHAHHPLH PHLMQEHMGT NVIVANTMPT HLSNGQMCEW 420
PRPRGQAATP ETPQPSPPGG SGSEPYKLLP GAVATIVKPL SAIPQPTITK QEVI 474 
Gene Ontology
 GO:0005737; C:cytoplasm; IDA:BHF-UCL.
 GO:0005654; C:nucleoplasm; TAS:Reactome.
 GO:0033613; F:activating transcription factor binding; ISS:BHF-UCL.
 GO:0005504; F:fatty acid binding; IDA:BHF-UCL.
 GO:0004879; F:ligand-activated sequence-specific DNA binding RNA polymerase II transcription factor activity; IEA:InterPro.
 GO:0042803; F:protein homodimerization activity; IDA:BHF-UCL.
 GO:0005102; F:receptor binding; IDA:BHF-UCL.
 GO:0001077; F:RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription; ISS:BHF-UCL.
 GO:0003705; F:RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity; IEA:Compara.
 GO:0043565; F:sequence-specific DNA binding; IEA:Compara.
 GO:0003707; F:steroid hormone receptor activity; IEA:InterPro.
 GO:0044212; F:transcription regulatory region DNA binding; IDA:BHF-UCL.
 GO:0008270; F:zinc ion binding; IEA:InterPro.
 GO:0007596; P:blood coagulation; IDA:BHF-UCL.
 GO:0031018; P:endocrine pancreas development; TAS:Reactome.
 GO:0042593; P:glucose homeostasis; ISS:BHF-UCL.
 GO:0030522; P:intracellular receptor signaling pathway; IEA:GOC.
 GO:0006629; P:lipid metabolic process; IEA:Compara.
 GO:0030308; P:negative regulation of cell growth; IMP:BHF-UCL.
 GO:0008285; P:negative regulation of cell proliferation; IMP:BHF-UCL.
 GO:0006591; P:ornithine metabolic process; IMP:BHF-UCL.
 GO:0055091; P:phospholipid homeostasis; ISS:BHF-UCL.
 GO:2000189; P:positive regulation of cholesterol homeostasis; ISS:BHF-UCL.
 GO:0010470; P:regulation of gastrulation; IEA:Compara.
 GO:0060398; P:regulation of growth hormone receptor signaling pathway; NAS:BHF-UCL.
 GO:0050796; P:regulation of insulin secretion; ISS:BHF-UCL.
 GO:0019216; P:regulation of lipid metabolic process; IDA:BHF-UCL.
 GO:0009749; P:response to glucose stimulus; ISS:BHF-UCL.
 GO:0007548; P:sex differentiation; IEA:Compara.
 GO:0023019; P:signal transduction involved in regulation of gene expression; IEA:Compara.
 GO:0060395; P:SMAD protein signal transduction; IEA:Compara.
 GO:0006367; P:transcription initiation from RNA polymerase II promoter; TAS:Reactome.
 GO:0070328; P:triglyceride homeostasis; ISS:BHF-UCL.
 GO:0006805; P:xenobiotic metabolic process; IMP:BHF-UCL. 
Interpro
 IPR003068; COUP_TF.
 IPR008946; Nucl_hormone_rcpt_ligand-bd.
 IPR000536; Nucl_hrmn_rcpt_lig-bd_core.
 IPR001723; Str_hrmn_rcpt.
 IPR001628; Znf_hrmn_rcpt.
 IPR013088; Znf_NHR/GATA. 
Pfam
 PF00104; Hormone_recep
 PF00105; zf-C4 
SMART
 SM00430; HOLI
 SM00399; ZnF_C4 
PROSITE
 PS00031; NUCLEAR_REC_DBD_1
 PS51030; NUCLEAR_REC_DBD_2 
PRINTS
 PR01282; COUPTNFACTOR.
 PR00398; STRDHORMONER.
 PR00047; STROIDFINGER.