CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-030868
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Eukaryotic translation initiation factor 2-alpha kinase 1 
Protein Synonyms/Alias
 cDNA FLJ58724, highly similar to Eukaryotic translation initiation factor2-alpha kinase 1 (EC 2.7.11.1) 
Gene Name
 EIF2AK1 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
51EELAILGKGGYGRVYubiquitination[1, 2, 3]
179DTENQNNKSVKYTTNubiquitination[1, 2, 3]
334HGPDQQVKIGDFGLAubiquitination[4]
348ACTDILQKNTDWTNRubiquitination[2, 4, 5]
434KRCPVQAKYIQHLTRubiquitination[2]
485KEIAELKKQLNLLSQubiquitination[2]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [4] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [5] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302] 
Functional Description
  
Sequence Annotation
  
Keyword
 ATP-binding; Complete proteome; Initiation factor; Kinase; Nucleotide-binding; Protein biosynthesis; Reference proteome; Serine/threonine-protein kinase; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 577 AA 
Protein Sequence
MNQTHFVQDR CLSDPCEDIS RIQKIRSREV ALEAQTSRYL NEFEELAILG KGGYGRVYKV 60
RNKLDGQYYA IKKILIKGAT KTVCMKVLRE VKVLAGLQHP NIVGYHTAWI EHVHVIQPRA 120
DRAAIELPSL EVLSDQEEDR EQCGVKNDES SSSSIIFAEP TPEKEKRFGE SDTENQNNKS 180
VKYTTNLVIR ESGELESTLE LQENGLAGLS ASSIVEQQLP LRRNSHLEES FTSTEESSEE 240
NVNFLGQTEA QYHLMLHIQM QLCELSLWDW IVERNKRGRE YVDESACPYV MANVATKIFQ 300
ELVEGVFYIH NMGIVHRDLK PRNIFLHGPD QQVKIGDFGL ACTDILQKNT DWTNRNGKRT 360
PTHTSRVGTC LYASPEQLEG SEYDAKSDMY SLGVVLLELF QPFGTEMERA EVLTGLRTGQ 420
LPESLRKRCP VQAKYIQHLT RRNSSQRPSA IQLLQSELFQ NSGNVNLTLQ MKIIEQEKEI 480
AELKKQLNLL SQDKGFSVIP KPHPCKALYS APQMISRQLR SLSCLCPALF PGTSSFIVAL 540
SSPANLYIPQ RQRSDELVFE SQKGSAMELA VITVDGV 577 
Gene Ontology
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0004674; F:protein serine/threonine kinase activity; IEA:UniProtKB-KW.
 GO:0003743; F:translation initiation factor activity; IEA:UniProtKB-KW.
 GO:0006950; P:response to stress; IEA:InterPro. 
Interpro
 IPR015516; Haem-reg_EIF2A_Kinase.
 IPR011009; Kinase-like_dom.
 IPR000719; Prot_kinase_cat_dom.
 IPR017441; Protein_kinase_ATP_BS.
 IPR008271; Ser/Thr_kinase_AS. 
Pfam
 PF00069; Pkinase 
SMART
  
PROSITE
 PS00107; PROTEIN_KINASE_ATP
 PS50011; PROTEIN_KINASE_DOM
 PS00108; PROTEIN_KINASE_ST 
PRINTS