CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-024067
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Gamma-aminobutyric acid type B receptor subunit 1 
Protein Synonyms/Alias
 GABA-B receptor 1; GABA-B-R1; GABA-BR1; GABABR1; Gb1 
Gene Name
 Gabbr1 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Rattus norvegicus (Rat) 
NCBI Taxa ID
 10116 
Lysine Modification
Position
Peptide
Type
References
917TNNNEEEKSRLLEKEubiquitination[1]
935LEKIIAEKEERVSELubiquitination[1]
Reference
 [1] Synaptic protein ubiquitination in rat brain revealed by antibody-based ubiquitome analysis.
 Na CH, Jones DR, Yang Y, Wang X, Xu Y, Peng J.
 J Proteome Res. 2012 Sep 7;11(9):4722-32. [PMID: 22871113] 
Functional Description
 Receptor for GABA. Calcium is required for high affinity binding to GABA. The activity of this receptor is mediated by G- proteins that inhibit adenylyl cyclase activity, stimulates phospholipase A2, activates potassium channels, inactivates voltage-dependent calcium-channels and modulates inositol phospholipids hydrolysis. Plays a critical role in the fine-tuning of inhibitory synaptic transmission. Pre-synaptic GABA-B-R inhibit neurotransmitter release by down-regulating high-voltage activated calcium channels, whereas postsynaptic GABA-B-R decrease neuronal excitability by activating a prominent inwardly rectifying potassium (Kir) conductance that underlies the late inhibitory postsynaptic potentials. Not only implicated in synaptic inhibition but also in hippocampal long-term potentiation, slow wave sleep, muscle relaxation and antinociception. 
Sequence Annotation
 DOMAIN 29 95 Sushi 1.
 DOMAIN 97 158 Sushi 2.
 REGION 918 946 Interaction with ATF4.
 MOD_RES 960 960 Phosphothreonine (By similarity).
 CARBOHYD 23 23 N-linked (GlcNAc...) (Potential).
 CARBOHYD 83 83 N-linked (GlcNAc...) (Potential).
 CARBOHYD 408 408 N-linked (GlcNAc...) (Potential).
 CARBOHYD 439 439 N-linked (GlcNAc...) (Potential).
 CARBOHYD 481 481 N-linked (GlcNAc...) (Potential).
 CARBOHYD 501 501 N-linked (GlcNAc...) (Potential).
 CARBOHYD 513 513 N-linked (GlcNAc...) (Potential).
 DISULFID 99 144
 DISULFID 130 156  
Keyword
 3D-structure; Alternative splicing; Cell junction; Cell membrane; Coiled coil; Complete proteome; Disulfide bond; G-protein coupled receptor; Glycoprotein; Membrane; Phosphoprotein; Postsynaptic cell membrane; Receptor; Reference proteome; Repeat; Signal; Synapse; Transducer; Transmembrane; Transmembrane helix. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 991 AA 
Protein Sequence
MLLLLLVPLF LRPLGAGGAQ TPNATSEGCQ IIHPPWEGGI RYRGLTRDQV KAINFLPVDY 60
EIEYVCRGER EVVGPKVRKC LANGSWTDMD TPSRCVRICS KSYLTLENGK VFLTGGDLPA 120
LDGARVEFRC DPDFHLVGSS RSVCSQGQWS TPKPHCQVNR TPHSERRAVY IGALFPMSGG 180
WPGGQACQPA VEMALEDVNS RRDILPDYEL KLIHHDSKCD PGQATKYLYE LLYNDPIKII 240
LMPGCSSVST LVAEAARMWN LIVLSYGSSS PALSNRQRFP TFFRTHPSAT LHNPTRVKLF 300
EKWGWKKIAT IQQTTEVFTS TLDDLEERVK EAGIEITFRQ SFFSDPAVPV KNLKRQDARI 360
IVGLFYETEA RKVFCEVYKE RLFGKKYVWF LIGWYADNWF KTYDPSINCT VEEMTEAVEG 420
HITTEIVMLN PANTRSISNM TSQEFVEKLT KRLKRHPEET GGFQEAPLAY DAIWALALAL 480
NKTSGGGGRS GVRLEDFNYN NQTITDQIYR AMNSSSFEGV SGHVVFDASG SRMAWTLIEQ 540
LQGGSYKKIG YYDSTKDDLS WSKTDKWIGG SPPADQTLVI KTFRFLSQKL FISVSVLSSL 600
GIVLAVVCLS FNIYNSHVRY IQNSQPNLNN LTAVGCSLAL AAVFPLGLDG YHIGRSQFPF 660
VCQARLWLLG LGFSLGYGSM FTKIWWVHTV FTKKEEKKEW RKTLEPWKLY ATVGLLVGMD 720
VLTLAIWQIV DPLHRTIETF AKEEPKEDID VSILPQLEHC SSKKMNTWLG ELWSFAVSSD 780
VQRRATVGGD SPICVWPAPE SIFYGYKGLL LLLGIFLAYE TKSVSTEKIN DHRAVGMAIY 840
NVAVLCLITA PVTMILSSQQ DAAFAFASLA IVFSSYITLV VLFVPKMRRL ITRGEWQSET 900
QDTMKTGSST NNNEEEKSRL LEKENRELEK IIAEKEERVS ELRHQLQSRQ QLRSRRHPPT 960
PPDPSGGLPR GPSEPPDRLS CDGSRVHLLY K 991 
Gene Ontology
 GO:0030673; C:axolemma; IDA:RGD.
 GO:0030054; C:cell junction; IEA:UniProtKB-KW.
 GO:0043198; C:dendritic shaft; IDA:RGD.
 GO:0043197; C:dendritic spine; IDA:RGD.
 GO:0005789; C:endoplasmic reticulum membrane; IDA:RGD.
 GO:0005615; C:extracellular space; IDA:RGD.
 GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
 GO:0045121; C:membrane raft; IDA:RGD.
 GO:0031966; C:mitochondrial membrane; IDA:RGD.
 GO:0043025; C:neuronal cell body; IDA:RGD.
 GO:0045211; C:postsynaptic membrane; IEA:UniProtKB-SubCell.
 GO:0008021; C:synaptic vesicle; IDA:RGD.
 GO:0004965; F:G-protein coupled GABA receptor activity; IDA:RGD.
 GO:0007194; P:negative regulation of adenylate cyclase activity; IDA:MGI.
 GO:0008285; P:negative regulation of cell proliferation; IMP:RGD.
 GO:0033602; P:negative regulation of dopamine secretion; IDA:RGD.
 GO:0032811; P:negative regulation of epinephrine secretion; IMP:RGD.
 GO:0014053; P:negative regulation of gamma-aminobutyric acid secretion; IMP:RGD.
 GO:0050805; P:negative regulation of synaptic transmission; IDA:RGD.
 GO:0001649; P:osteoblast differentiation; IMP:RGD.
 GO:0014049; P:positive regulation of glutamate secretion; IMP:RGD.
 GO:0060124; P:positive regulation of growth hormone secretion; IMP:RGD.
 GO:0045471; P:response to ethanol; IEP:RGD.
 GO:0035094; P:response to nicotine; IEP:RGD. 
Interpro
 IPR001828; ANF_lig-bd_rcpt.
 IPR017978; GPCR_3_C.
 IPR002455; GPCR_3_GABA_rcpt_B.
 IPR002456; GPCR_3_GABA_rcpt_B1.
 IPR000436; Sushi_SCR_CCP. 
Pfam
 PF00003; 7tm_3
 PF01094; ANF_receptor
 PF00084; Sushi 
SMART
 SM00032; CCP 
PROSITE
 PS00979; G_PROTEIN_RECEP_F3_1
 PS00980; G_PROTEIN_RECEP_F3_2
 PS00981; G_PROTEIN_RECEP_F3_3
 PS50259; G_PROTEIN_RECEP_F3_4
 PS50923; SUSHI 
PRINTS
 PR01177; GABAB1RECPTR.
 PR01176; GABABRECEPTR.