CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-006903
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Antagonist of mitotic exit network protein 1 
Protein Synonyms/Alias
 Chromosome stability protein 13; Increased copper-sensitivity protein 4 
Gene Name
 AMN1 
Gene Synonyms/Alias
 CST13; ICS4; YBR158W; YBR1208 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
294PSHFILHKLHQVTQPubiquitination[1]
315RMECQNLKWLEFYVCubiquitination[1]
347KLIIPGNKNIDDNFLubiquitination[1]
365SQSIPNLKHLVLRACubiquitination[1]
389CIALNCPKLKTFNIGubiquitination[1]
442EFARLNGKNVERLSLubiquitination[1]
482LEIRNLDKITDVRHFubiquitination[1]
Reference
 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301] 
Functional Description
 Negative regulator of the mitotic exit network (MEN), required for multiple cell cycle checkpoints. Acts in the daughter cell to inhibit the mitotic exit pathway once MEN has executed its function. Through its binding ability to TEM1, interferes with the TEM1-CDC5 association, required for CDC5 kinase activation and MEN activation. Required for daughter cell separation and chromosome stability. Involved in copper sensitivity. 
Sequence Annotation
  
Keyword
 Cell cycle; Cell division; Complete proteome; Cytoplasm; Mitosis; Nucleus; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 549 AA 
Protein Sequence
MKLERVSSNG SFKRGRDIQS LESPCTRPLK KMSPSPSFTS LKMEKPFKDI VRKYGGHLHQ 60
SSYNPGSSKV ELVRPDLSLK TDQSFLQSSV QTTPNKKSCN EYLSTPEATP LKNTATENAW 120
ATSRVVSASS LSIVTPTEIK NILVDEFSEL KLGQPLTAQH QRSHAVFEIP EIVENIIKMI 180
VSLESANIPK ERPCLRRNPQ SYEHSLLMYK DEERAKKAWS AAQQLRDPPL VGHKEKKQGA 240
LFSCMMVNRL WLNVTRPFLF KSLHFKSVHN FKEFLRTSQE TTQVMRPSHF ILHKLHQVTQ 300
PDIERLSRME CQNLKWLEFY VCPRITPPLS WFDNLHKLEK LIIPGNKNID DNFLLRLSQS 360
IPNLKHLVLR ACDNVSDSGV VCIALNCPKL KTFNIGRHRR GNLITSVSLV ALGKYTQVET 420
VGFAGCDVDD AGIWEFARLN GKNVERLSLN SCRLLTDYSL PILFALNSFP NLAVLEIRNL 480
DKITDVRHFV KYNLWKKSLD APILIEACER ITKLIDQEEN RVKRINSLVA LKDMTAWVNA 540
DDEIENNVD 549 
Gene Ontology
 GO:0005933; C:cellular bud; IDA:SGD.
 GO:0005737; C:cytoplasm; IDA:SGD.
 GO:0005634; C:nucleus; IDA:SGD.
 GO:0051301; P:cell division; IEA:UniProtKB-KW.
 GO:0007067; P:mitosis; IEA:UniProtKB-KW.
 GO:0007093; P:mitotic cell cycle checkpoint; IMP:SGD.
 GO:0001100; P:negative regulation of exit from mitosis; IMP:SGD. 
Interpro
  
Pfam
  
SMART
  
PROSITE
  
PRINTS