| Tag | Content |
|---|
| CPLM ID | CPLM-011900 |
UniProt Accession | |
Genbank Protein ID | |
Genbank Nucleotide ID | |
Protein Name | Sterol regulatory element-binding protein cleavage-activating protein |
Protein Synonyms/Alias | SCAP; SREBP cleavage-activating protein |
Gene Name | SCAP |
Gene Synonyms/Alias | KIAA0199; PSEC0227 |
Created Date | July 27, 2013 |
Organism | Homo sapiens (Human) |
NCBI Taxa ID | 9606 |
Lysine Modification | Position | Peptide | Type | References |
|---|
| 378 | TPVDLEVKLRIAQGL | ubiquitination | [1, 2] | | 454 | MELADLNKRLPPEAC | ubiquitination | [1, 2] | | 842 | ERLSDGGKAGPEEPG | ubiquitination | [1, 2] |
|
Reference | [1] Systematic and quantitative assessment of the ubiquitin-modified proteome. Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP. Mol Cell. 2011 Oct 21;44(2):325-40. [ PMID: 21906983] [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments. Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA. Mol Cell Proteomics. 2013 Mar;12(3):825-31. [ PMID: 23266961] |
Functional Description | Escort protein required for cholesterol as well as lipid homeostasis. Regulates export of the SCAP/SREBF complex from the ER upon low cholesterol. Formation of a ternary complex with INSIG at high sterol concentrations leads to masking of an ER-export signal in SCAP and retention of the complex in the ER. Low sterol concentrations trigger release of INSIG, a conformational change in the SSC domain of SCAP, unmasking of the ER export signal, recruitment into COPII-coated vesicles, transport to the Golgi complex, proteolytic cleavage of SREBF in the Golgi, release of the transcription factor fragment of SREBF from the membrane, its import into the nucleus and up-regulation of LDLR, INSIG1 and the mevalonate pathway (By similarity). |
Sequence Annotation | DOMAIN 284 442 SSD.
REPEAT 771 811 WD 1.
REPEAT 952 1002 WD 2.
REPEAT 1005 1042 WD 3.
REPEAT 1077 1114 WD 4.
REPEAT 1117 1155 WD 5.
REPEAT 1158 1195 WD 6.
REPEAT 1197 1235 WD 7.
REGION 447 452 ER export signal (By similarity).
REGION 731 1279 Interaction with SREBF2 (By similarity).
MOD_RES 907 907 Phosphoserine.
CARBOHYD 263 263 N-linked (GlcNAc...) (Potential).
CARBOHYD 590 590 N-linked (GlcNAc...) (Potential).
CARBOHYD 641 641 N-linked (GlcNAc...) (Potential). |
Keyword | Alternative splicing; Cholesterol metabolism; Complete proteome; Cytoplasmic vesicle; Endoplasmic reticulum; Glycoprotein; Golgi apparatus; Lipid metabolism; Membrane; Phosphoprotein; Polymorphism; Reference proteome; Repeat; Steroid metabolism; Sterol metabolism; Transmembrane; Transmembrane helix; WD repeat. |
Sequence Source | UniProt (SWISSPROT/TrEMBL); GenBank; EMBL |
Protein Length | 1279 AA |
Protein Sequence | MTLTERLREK ISRAFYNHGL LCASYPIPII LFTGFCILAC CYPLLKLPLP GTGPVEFTTP 60
VKDYSPPPVD SDRKQGEPTE QPEWYVGAPV AYVQQIFVKS SVFPWHKNLL AVDVFRSPLS 120
RAFQLVEEIR NHVLRDSSGI RSLEELCLQV TDLLPGLRKL RNLLPEHGCL LLSPGNFWQN 180
DWERFHADPD IIGTIHQHEP KTLQTSATLK DLLFGVPGKY SGVSLYTRKR MVSYTITLVF 240
QHYHAKFLGS LRARLMLLHP SPNCSLRAES LVHVHFKEEI GVAELIPLVT TYIILFAYIY 300
FSTRKIDMVK SKWGLALAAV VTVLSSLLMS VGLCTLFGLT PTLNGGEIFP YLVVVIGLEN 360
VLVLTKSVVS TPVDLEVKLR IAQGLSSESW SIMKNMATEL GIILIGYFTL VPAIQEFCLF 420
AVVGLVSDFF LQMLFFTTVL SIDIRRMELA DLNKRLPPEA CLPSAKPVGQ PTRYERQLAV 480
RPSTPHTITL QPSSFRNLRL PKRLRVVYFL ARTRLAQRLI MAGTVVWIGI LVYTDPAGLR 540
NYLAAQVTEQ SPLGEGALAP MPVPSGMLPP SHPDPAFSIF PPDAPKLPEN QTSPGESPER 600
GGPAEVVHDS PVPEVTWGPE DEELWRKLSF RHWPTLFSYY NITLAKRYIS LLPVIPVTLR 660
LNPREALEGR HPQDGRSAWP PPGPIPAGHW EAGPKGPGGV QAHGDVTLYK VAALGLATGI 720
VLVLLLLCLY RVLCPRNYGQ LGGGPGRRRR GELPCDDYGY APPETEIVPL VLRGHLMDIE 780
CLASDGMLLV SCCLAGHVCV WDAQTGDCLT RIPRPGRQRR DSGVGSGLEA QESWERLSDG 840
GKAGPEEPGD SPPLRHRPRG PPPPSLFGDQ PDLTCLIDTN FSAQPRSSQP TQPEPRHRAV 900
CGRSRDSPGY DFSCLVQRVY QEEGLAAVCT PALRPPSPGP VLSQAPEDEG GSPEKGSPSL 960
AWAPSAEGSI WSLELQGNLI VVGRSSGRLE VWDAIEGVLC CSSEEVSSGI TALVFLDKRI 1020
VAARLNGSLD FFSLETHTAL SPLQFRGTPG RGSSPASPVY SSSDTVACHL THTVPCAHQK 1080
PITALKAAAG RLVTGSQDHT LRVFRLEDSC CLFTLQGHSG AITTVYIDQT MVLASGGQDG 1140
AICLWDVLTG SRVSHVFAHR GDVTSLTCTT SCVISSGLDD LISIWDRSTG IKFYSIQQDL 1200
GCGASLGVIS DNLLVTGGQG CVSFWDLNYG DLLQTVYLGK NSEAQPARQI LVLDNAAIVC 1260
NFGSELSLVY VPSVLEKLD 1279 |
Gene Ontology | GO:0005783; C:endoplasmic reticulum; NAS:UniProtKB. GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell. GO:0012507; C:ER to Golgi transport vesicle membrane; IEA:UniProtKB-SubCell. GO:0000139; C:Golgi membrane; TAS:Reactome. GO:0016021; C:integral to membrane; NAS:UniProtKB. GO:0043234; C:protein complex; IEA:Compara. GO:0051082; F:unfolded protein binding; NAS:UniProtKB. GO:0007568; P:aging; IEA:Compara. GO:0008203; P:cholesterol metabolic process; IEA:UniProtKB-KW. GO:0045541; P:negative regulation of cholesterol biosynthetic process; NAS:UniProtKB. GO:0045716; P:positive regulation of low-density lipoprotein particle receptor biosynthetic process; NAS:UniProtKB. GO:0042304; P:regulation of fatty acid biosynthetic process; IEA:Compara. GO:0001666; P:response to hypoxia; IEA:Compara. GO:0032868; P:response to insulin stimulus; IEA:Compara. GO:0044281; P:small molecule metabolic process; TAS:Reactome. GO:0032933; P:SREBP signaling pathway; IMP:UniProtKB. |
Interpro | |
Pfam | |
SMART | |
PROSITE | |
PRINTS | |