UniProt Accession

 DHRS4_HUMAN; Q9BTZ2; B2RB10; B7WNS9; D3YTB8; E2QRL8; O95162; Q20CR0; Q2LC19; Q2LE81; Q58IU4; Q6E0Y1; Q6UWU3; Q71UQ6; Q8TD03; Q9H3N5; Q9NV08 

Genbank Protein ID

 AF044127; AB045131; AY071856; AF064256; AY616182; DQ344810; AY943857; DQ325464; DQ338571; AK001870; AK314448; AY358638; AL136419; BC003019 

Genbank Nucleotide ID

 AAD02292.1; BAB18775.1; AAL61824.2; AAQ13444.1; AAT70757.1; ABD75823.1; AAX49568.1; ABC61320.1; ABC61321.1; BAA91953.1; BAG37057.1; AAQ89001.1; AAH03019.1 

Protein Name

 Dehydrogenase/reductase SDR family member 4 

Protein Synonyms/Alias

 NADPH-dependent carbonyl reductase/NADP-retinol dehydrogenase; CR; PHCR; NADPH-dependent retinol dehydrogenase/reductase; NRDR; humNRDR; Peroxisomal short-chain alcohol dehydrogenase; PSCD; SCAD-SRL; Short-chain dehydrogenase/reductase family member 4 

Gene Name

 DHRS4 

Gene Synonyms/Alias

 UNQ851/PRO1800 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
65	HVVVSSRKQQNVDQA	ubiquitination	[1]
150	KAPALMTKAVVPEME	ubiquitination	[2]
194	TALLGLTKTLAIELA	ubiquitination	[2, 3]
216	CLAPGLIKTSFSRML	ubiquitination	[2]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [3] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302] 

Functional Description

 Reduces all-trans-retinal and 9-cis retinal. Can also catalyze the oxidation of all-trans-retinol with NADP as co- factor, but with much lower efficiency. Reduces alkyl phenyl ketones and alpha-dicarbonyl compounds with aromatic rings, such as pyrimidine-4-aldehyde, 3-benzoylpyridine, 4-benzoylpyridine, menadione and 4-hexanoylpyridine. Has no activity towards aliphatic aldehydes and ketones (By similarity). 

Sequence Annotation

 NP_BIND 36 60 NADP (By similarity).
 MOTIF 276 278 Microbody targeting signal.
 ACT_SITE 182 182 Proton acceptor (By similarity).
 BINDING 169 169 Substrate (By similarity).  

Keyword

 3D-structure; Alternative splicing; Complete proteome; NADP; Nucleus; Oxidoreductase; Peroxisome; Phosphoprotein; Polymorphism; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 278 AA 

Protein Sequence

Gene Ontology

 GO:0005739; C:mitochondrion; ISS:UniProtKB.
 GO:0005634; C:nucleus; IDA:UniProtKB.
 GO:0005778; C:peroxisomal membrane; IDA:UniProtKB.
 GO:0000253; F:3-keto sterol reductase activity; IDA:UniProtKB.
 GO:0018455; F:alcohol dehydrogenase [NAD(P)+] activity; IDA:UniProtKB.
 GO:0004090; F:carbonyl reductase (NADPH) activity; IDA:UniProtKB.
 GO:0016655; F:oxidoreductase activity, acting on NAD(P)H, quinone or similar compound as acceptor; IDA:UniProtKB.
 GO:0006066; P:alcohol metabolic process; IDA:UniProtKB.
 GO:0042180; P:cellular ketone metabolic process; IDA:UniProtKB.
 GO:0051262; P:protein tetramerization; IDA:UniProtKB.
 GO:0008202; P:steroid metabolic process; IDA:UniProtKB. 

Interpro

 IPR002198; DH_sc/Rdtase_SDR.
 IPR027052; DHRS2/4.
 IPR002347; Glc/ribitol_DH.
 IPR016040; NAD(P)-bd_dom.
 IPR020904; Sc_DH/Rdtase_CS. 

Pfam

  

SMART

  

PROSITE

 PS00061; ADH_SHORT 

PRINTS

 PR00081; GDHRDH.
 PR00080; SDRFAMILY.