UniProt Accession

 CBS_YEAST; P32582; D6VUT5; Q05177; Q27JK1; Q27JK4 

Genbank Protein ID

 X72922; D16502; L14578; DQ393806; DQ393807; DQ393808; DQ393809; X85807; Z72940; BK006941 

Genbank Nucleotide ID

 CAA51426.1; BAA03952.1; AAC37401.1; ABD57960.1; ABD57961.1; ABD57962.1; ABD57963.1; CAA59812.1; CAA97169.1; DAA08246.1 

Protein Name

 Cystathionine beta-synthase 

Protein Synonyms/Alias

 Beta-thionase; Serine sulfhydrase; Sulfur transfer protein 4 

Gene Name

 CYS4 

Gene Synonyms/Alias

 STR4; YGR155W; G6667 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
27	NTPLIALKKLPKALG	acetylation	[1]
53	YNPGGSIKDRIAKSM	ubiquitination	[2]
58	SIKDRIAKSMVEEAE	ubiquitination	[2, 3]
96	LALIGAIKGYRTIIT	ubiquitination	[2]
112	PEKMSNEKVSVLKAL	ubiquitination	[2]
117	NEKVSVLKALGAEII	ubiquitination	[2]
206	GTISGISKYLKEQND	acetylation	[1]
235	AQPENLNKTDITDYK	ubiquitination	[4]
258	VPQVLDRKLIDVWYK	acetylation	[1]
273	TDDKPSFKYARQLIS	acetylation	[1]
327	SIRSYLTKFVDDEWL	ubiquitination	[2]
335	FVDDEWLKKNNLWDD	acetylation	[1]
352	LARFDSSKLEASTTK	acetylation	[1]
359	KLEASTTKYADVFGN	ubiquitination	[2]
370	VFGNATVKDLHLKPV	acetylation	[1]
375	TVKDLHLKPVVSVKE	acetylation	[1]
391	AKVTDVIKILKDNGF	acetylation	[1]
394	TDVIKILKDNGFDQL	acetylation	[1]
422	TLSELLRKLSINNSN	ubiquitination	[2]
443	GKYLDFKKLNNFNDV	ubiquitination	[2]
465	SGKKKFIKFDENSKL	acetylation	[1]
471	IKFDENSKLSDLNRF	acetylation	[1]
481	DLNRFFEKNSSAVIT	acetylation	[1]
492	AVITDGLKPIHIVTK	acetylation	[1]

Reference

 [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]
 [2] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [3] Identification, analysis, and prediction of protein ubiquitination sites.
 Radivojac P, Vacic V, Haynes C, Cocklin RR, Mohan A, Heyen JW, Goebl MG, Iakoucheva LM.
 Proteins. 2010 Feb 1;78(2):365-80. [PMID: 19722269]
 [4] Systematic approach for validating the ubiquitinated proteome.
 Seyfried NT, Xu P, Duong DM, Cheng D, Hanfelt J, Peng J.
 Anal Chem. 2008 Jun 1;80(11):4161-9. [PMID: 18433149] 

Functional Description

  

Sequence Annotation

 DOMAIN 373 432 CBS.
 REGION 196 200 Pyridoxal phosphate binding (By
 BINDING 84 84 Pyridoxal phosphate (By similarity).
 BINDING 289 289 Pyridoxal phosphate (By similarity).
 MOD_RES 53 53 N6-(pyridoxal phosphate)lysine (By
 MOD_RES 134 134 Phosphoserine.
 MOD_RES 350 350 Phosphoserine.
 MOD_RES 424 424 Phosphoserine.  

Keyword

 Amino-acid biosynthesis; CBS domain; Complete proteome; Cysteine biosynthesis; Lyase; Phosphoprotein; Pyridoxal phosphate; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 507 AA 

Protein Sequence

Gene Ontology

 GO:0005739; C:mitochondrion; IDA:SGD.
 GO:0004122; F:cystathionine beta-synthase activity; IDA:SGD.
 GO:0006535; P:cysteine biosynthetic process from serine; IDA:SGD.
 GO:0019343; P:cysteine biosynthetic process via cystathionine; IMP:SGD.
 GO:0070814; P:hydrogen sulfide biosynthetic process; IDA:SGD.
 GO:0019346; P:transsulfuration; IMP:SGD.
 GO:0007089; P:traversing start control point of mitotic cell cycle; IMP:SGD. 

Interpro

 IPR001216; Cys_synth_BS.
 IPR005857; Cysta_beta_synth.
 IPR000644; Cysta_beta_synth_core.
 IPR001926; Trp_syn_b_sub_like_PLP_eny_SF. 

Pfam

 PF00571; CBS
 PF00291; PALP 

SMART

 SM00116; CBS 

PROSITE

 PS51371; CBS
 PS00901; CYS_SYNTHASE 

PRINTS