UniProt Accession

 DYR_ECOLI; P0ABQ4; P00379 

Genbank Protein ID

 J01609; X05108; U00096; AP009048 

Genbank Nucleotide ID

 AAA87976.1; CAA28755.1; AAC73159.1; BAB96616.1 

Protein Name

 Dihydrofolate reductase 

Protein Synonyms/Alias

  

Gene Name

 folA 

Gene Synonyms/Alias

 tmrA; b0048; JW0047 

Created Date

 July 27, 2013 

Organism

 Escherichia coli (strain K12) 

NCBI Taxa ID

 83333 

Lysine Modification

Position	Peptide	Type	References
32	PADLAWFKRNTLNKP	acetylation	[1]
106	VYEQFLPKAQKLYLT	acetylation	[1]

Reference

 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618] 

Functional Description

 Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis. 

Sequence Annotation

 DOMAIN 1 158 DHFR.
 NP_BIND 13 19 NADP.
 NP_BIND 45 46 NADP.
 NP_BIND 63 64 NADP.
 NP_BIND 95 102 NADP.
 BINDING 5 5 Substrate; via carbonyl oxygen.
 BINDING 7 7 NADP; via amide nitrogen and carbonyl
 BINDING 27 27 Substrate.
 BINDING 52 52 Substrate.
 BINDING 57 57 Substrate.
 BINDING 76 76 NADP; via carbonyl oxygen.
 BINDING 113 113 Substrate.  

Keyword

 3D-structure; Antibiotic resistance; Complete proteome; Direct protein sequencing; Methotrexate resistance; NADP; One-carbon metabolism; Oxidoreductase; Reference proteome; Trimethoprim resistance. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 159 AA 

Protein Sequence

Gene Ontology

 GO:0004146; F:dihydrofolate reductase activity; IDA:EcoCyc.
 GO:0050661; F:NADP binding; IEA:InterPro.
 GO:0009257; P:10-formyltetrahydrofolate biosynthetic process; IGI:EcoCyc.
 GO:0046656; P:folic acid biosynthetic process; IGI:EcoCyc.
 GO:0006545; P:glycine biosynthetic process; IEA:InterPro.
 GO:0009165; P:nucleotide biosynthetic process; IEA:InterPro.
 GO:0006730; P:one-carbon metabolic process; IEA:UniProtKB-KW.
 GO:0046677; P:response to antibiotic; IEA:UniProtKB-KW.
 GO:0042493; P:response to drug; IDA:EcoliWiki.
 GO:0031427; P:response to methotrexate; IEA:UniProtKB-KW. 

Interpro

 IPR012259; DHFR.
 IPR024072; DHFR-like_dom.
 IPR017925; DHFR_CS.
 IPR001796; DHFR_dom. 

Pfam

 PF00186; DHFR_1 

SMART

  

PROSITE

 PS00075; DHFR_1
 PS51330; DHFR_2 

PRINTS

 PR00070; DHFR.