UniProt Accession

 KU70_YEAST; P32807; D6W0B1; P32498; Q0P778; Q0P779; Q0P787; Q0P789; Q6B267 

Genbank Protein ID

 X70379; D15052; AM296304; AM296302; AM296301; AM296299; AM296298; AM296297; AM296296; AM296294; AM296292; AM296295; AM296303; AM296293; Z49704; AY692863; BK006946 

Genbank Nucleotide ID

 CAA49840.1; BAA03648.1; CAL36013.1; CAL36015.1; CAL36016.1; CAL36018.1; CAL36019.1; CAL36020.1; CAL36021.1; CAL36023.1; CAL36025.1; CAL36022.1; CAL36014.1; CAL36024.1; CAA89782.1; AAT92882.1; DAA10185.1 

Protein Name

 ATP-dependent DNA helicase II subunit 1 

Protein Synonyms/Alias

 ATP-dependent DNA helicase II subunit Ku70; High affinity DNA-binding factor subunit 1 

Gene Name

 YKU70 

Gene Synonyms/Alias

 HDF1; NES24; YMR284W; YM8021.10 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
406	RTLASLLKILRKKDK	ubiquitination	[1]

Reference

 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301] 

Functional Description

 Single stranded DNA-dependent ATP-dependent helicase. Involved in non-homologous end joining (NHEJ) DNA double strand break repair. DNA-binding is sequence-independent but has a high affinity to nicks in double stranded DNA and to the ends of duplex DNA. Binds to naturally occurring chromosomal ends, and therefore provides chromosomal end protection. Appears to have a role in recruitment of telomerase and CDC13 to the telomere and the subsequent telomere elongation. Required also for telomere recombination to repair telomeric ends in the absence of telomerase. KU70, of the KU70/KU80 heterodimer, binds to the stem loop of TLC1, the RNA component of telomerase. Involved in telomere maintenance. Interacts with telomeric repeats and subtelomeric sequences thereby controlling telomere length and protecting against subtelomeric rearrangement. Maintains telomeric chromatin, which is involved in silencing the expression of genes located at the telomere. Required for mating-type switching. 

Sequence Annotation

 DOMAIN 268 483 Ku.
 MOD_RES 370 370 Phosphoserine.
 MOD_RES 371 371 Phosphoserine.
 MOD_RES 372 372 Phosphoserine.  

Keyword

 ATP-binding; Chromosome; Complete proteome; Direct protein sequencing; DNA damage; DNA recombination; DNA repair; DNA-binding; Helicase; Hydrolase; Nucleotide-binding; Nucleus; Phosphoprotein; Reference proteome; Telomere; Ubl conjugation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 602 AA 

Protein Sequence

Gene Ontology

 GO:0043564; C:Ku70:Ku80 complex; IDA:SGD.
 GO:0005635; C:nuclear envelope; IDA:SGD.
 GO:0005724; C:nuclear telomeric heterochromatin; TAS:SGD.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0004003; F:ATP-dependent DNA helicase activity; IEA:InterPro.
 GO:0003684; F:damaged DNA binding; TAS:SGD.
 GO:0003723; F:RNA binding; IDA:SGD.
 GO:0042162; F:telomeric DNA binding; IEA:InterPro.
 GO:0006333; P:chromatin assembly or disassembly; IDA:SGD.
 GO:0030466; P:chromatin silencing at silent mating-type cassette; IMP:SGD.
 GO:0000727; P:double-strand break repair via break-induced replication; IMP:SGD.
 GO:0006303; P:double-strand break repair via nonhomologous end joining; IDA:SGD.
 GO:0000723; P:telomere maintenance; IMP:SGD. 

Interpro

 IPR006165; Ku70.
 IPR006164; Ku70/Ku80_beta-barrel_dom.
 IPR005160; Ku_C.
 IPR005161; Ku_N.
 IPR016194; SPOC_like_C_dom.
 IPR002035; VWF_A. 

Pfam

 PF02735; Ku
 PF03730; Ku_C
 PF03731; Ku_N 

SMART

 SM00559; Ku78 

PROSITE

  

PRINTS