UniProt Accession

 UBC3_YEAST; P14682; D6VS40 

Genbank Protein ID

 M21877; X84162; Z74350; Z49209; BK006938 

Genbank Nucleotide ID

 AAA35188.1; CAA58970.1; CAA98872.1; CAA89083.1; DAA11900.1 

Protein Name

 Ubiquitin-conjugating enzyme E2-34 kDa 

Protein Synonyms/Alias

 Cell division control protein 34; E3 ubiquitin ligase complex SCF subunit CDC34; Ubiquitin carrier protein; Ubiquitin-protein ligase 

Gene Name

 CDC34 

Gene Synonyms/Alias

 DNA6; UBC3; YDR054C; D4211; YD9609.08C 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
151	DAAVDYRKNPEQYKQ	ubiquitination	[1]
157	RKNPEQYKQRVKMEV	ubiquitination	[1]
161	EQYKQRVKMEVERSK	ubiquitination	[1]
168	KMEVERSKQDIPKGF	ubiquitination	[1]
189	SAYISQSKLDEPESN	ubiquitination	[1]

Reference

 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301] 

Functional Description

 Catalyzes the covalent attachment of ubiquitin to other proteins. Capable, in vitro, to ubiquitinate histone H2A. 

Sequence Annotation

 ACT_SITE 95 95 Glycyl thioester intermediate (By
 MOD_RES 186 186 Phosphoserine.
 MOD_RES 282 282 Phosphoserine.
 MOD_RES 292 292 Phosphoserine.  

Keyword

 ATP-binding; Cell cycle; Cell division; Complete proteome; Cytoplasm; DNA replication; Ligase; Nucleotide-binding; Nucleus; Phosphoprotein; Reference proteome; Ubl conjugation pathway. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 295 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IDA:SGD.
 GO:0005634; C:nucleus; IDA:SGD.
 GO:0019005; C:SCF ubiquitin ligase complex; IDA:SGD.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0042803; F:protein homodimerization activity; IDA:SGD.
 GO:0004842; F:ubiquitin-protein ligase activity; IDA:SGD.
 GO:0051301; P:cell division; IEA:UniProtKB-KW.
 GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
 GO:0000082; P:G1/S transition of mitotic cell cycle; TAS:SGD.
 GO:0000086; P:G2/M transition of mitotic cell cycle; IGI:SGD.
 GO:0051865; P:protein autoubiquitination; IDA:SGD.
 GO:0000209; P:protein polyubiquitination; IDA:SGD.
 GO:0042787; P:protein ubiquitination involved in ubiquitin-dependent protein catabolic process; IDA:SGD.
 GO:0031146; P:SCF-dependent proteasomal ubiquitin-dependent protein catabolic process; IDA:SGD. 

Interpro

 IPR000608; UBQ-conjugat_E2.
 IPR023313; UBQ-conjugating_AS.
 IPR016135; UBQ-conjugating_enzyme/RWD. 

Pfam

 PF00179; UQ_con 

SMART

  

PROSITE

 PS00183; UBIQUITIN_CONJUGAT_1
 PS50127; UBIQUITIN_CONJUGAT_2 

PRINTS