CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-023731
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Acyl-coenzyme A thioesterase 9, mitochondrial 
Protein Synonyms/Alias
 Acyl-CoA thioesterase 9; Acyl-CoA thioester hydrolase 9 
Gene Name
 ACOT9 
Gene Synonyms/Alias
 CGI-16 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
59TNWRDHVKAMEERKLubiquitination[1]
65VKAMEERKLLHSFLAubiquitination[1]
73LLHSFLAKSQDGLPPubiquitination[1]
103SEPELREKYLTVQNTacetylation[2]
138HNKIHSAKMSPLSIVubiquitination[1]
157DKIDMCKKSLSPEQDacetylation[2]
211VARDSENKGPAFVNPubiquitination[3]
250FSSTSLLKMAPSAEEacetylation[2]
250FSSTSLLKMAPSAEEubiquitination[1]
272FLSTLDPKTISFRSRubiquitination[1]
407EVPLVFPKTYGESMLacetylation[2, 4, 5]
407EVPLVFPKTYGESMLubiquitination[6]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [4] Proteomic investigations reveal a role for RNA processing factor THRAP3 in the DNA damage response.
 Beli P, Lukashchuk N, Wagner SA, Weinert BT, Olsen JV, Baskcomb L, Mann M, Jackson SP, Choudhary C.
 Mol Cell. 2012 Apr 27;46(2):212-25. [PMID: 22424773]
 [5] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [6] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661] 
Functional Description
 Acyl-CoA thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH. Active on long chain acyl-CoAs. 
Sequence Annotation
 MOD_RES 103 103 N6-acetyllysine.  
Keyword
 Acetylation; Alternative splicing; Complete proteome; Hydrolase; Mitochondrion; Polymorphism; Reference proteome; Serine esterase; Transit peptide. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 439 AA 
Protein Sequence
MRRAALRLCA LGKGQLTPGR GLTQGPQNPK KQGIFHIHEV RDKLREIVGA STNWRDHVKA 60
MEERKLLHSF LAKSQDGLPP RRMKDSYIEV LLPLGSEPEL REKYLTVQNT VRFGRILEDL 120
DSLGVLICYM HNKIHSAKMS PLSIVTALVD KIDMCKKSLS PEQDIKFSGH VSWVGKTSME 180
VKMQMFQLHG DEFCPVLDAT FVMVARDSEN KGPAFVNPLI PESPEEEELF RQGELNKGRR 240
IAFSSTSLLK MAPSAEERTT IHEMFLSTLD PKTISFRSRV LPSNAVWMEN SKLKSLEICH 300
PQERNIFNRI FGGFLMRKAY ELAWATACSF GGSRPFVVAV DDIMFQKPVE VGSLLFLSSQ 360
VCFTQNNYIQ VRVHSEVASL QEKQHTTTNV FHFTFMSEKE VPLVFPKTYG ESMLYLDGQR 420
HFNSMSGPAT LRKDYLVEP 439 
Gene Ontology
 GO:0005739; C:mitochondrion; ISS:HGNC.
 GO:0003986; F:acetyl-CoA hydrolase activity; ISS:HGNC.
 GO:0004091; F:carboxylesterase activity; IEA:UniProtKB-KW.
 GO:0006637; P:acyl-CoA metabolic process; ISS:HGNC. 
Interpro
 IPR006683; Thioestr_supf. 
Pfam
 PF03061; 4HBT 
SMART
  
PROSITE
  
PRINTS