CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-012857
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 WD40 repeat-containing protein SMU1 
Protein Synonyms/Alias
 Smu-1 suppressor of mec-8 and unc-52 protein homolog 
Gene Name
 SMU1 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
19RLIMQYLKENSLHRAubiquitination[1, 2]
107TDPMIMLKQTQPERYubiquitination[2, 3]
194FRGKAAVKDVEEEKFubiquitination[2, 3, 4]
200VKDVEEEKFPTQLSRubiquitination[1, 2, 3]
214RHIKFGQKSHVECARubiquitination[2]
287ATGAQDGKIKVWKIQubiquitination[2, 3]
289GAQDGKIKVWKIQSGubiquitination[2]
292DGKIKVWKIQSGQCLubiquitination[2]
343LKSGKTLKEFRGHSSubiquitination[2]
379TVKIWNMKTTECSNTubiquitination[1, 2]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [4] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724] 
Functional Description
  
Sequence Annotation
 DOMAIN 6 38 LisH.
 DOMAIN 40 92 CTLH.
 REPEAT 212 253 WD 1.
 REPEAT 262 303 WD 2.
 REPEAT 305 346 WD 3.
 REPEAT 347 386 WD 4.
 REPEAT 388 426 WD 5.
 REPEAT 482 513 WD 6.
 MOD_RES 2 2 N-acetylserine.  
Keyword
 Acetylation; Complete proteome; Cytoplasm; Direct protein sequencing; Nucleus; Reference proteome; Repeat; WD repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 513 AA 
Protein Sequence
MSIEIESSDV IRLIMQYLKE NSLHRALATL QEETTVSLNT VDSIESFVAD INSGHWDTVL 60
QAIQSLKLPD KTLIDLYEQV VLELIELREL GAARSLLRQT DPMIMLKQTQ PERYIHLENL 120
LARSYFDPRE AYPDGSSKEK RRAAIAQALA GEVSVVPPSR LMALLGQALK WQQHQGLLPP 180
GMTIDLFRGK AAVKDVEEEK FPTQLSRHIK FGQKSHVECA RFSPDGQYLV TGSVDGFIEV 240
WNFTTGKIRK DLKYQAQDNF MMMDDAVLCM CFSRDTEMLA TGAQDGKIKV WKIQSGQCLR 300
RFERAHSKGV TCLSFSKDSS QILSASFDQT IRIHGLKSGK TLKEFRGHSS FVNEATFTQD 360
GHYIISASSD GTVKIWNMKT TECSNTFKSL GSTAGTDITV NSVILLPKNP EHFVVCNRSN 420
TVVIMNMQGQ IVRSFSSGKR EGGDFVCCAL SPRGEWIYCV GEDFVLYCFS TVTGKLERTL 480
TVHEKDVIGI AHHPHQNLIA TYSEDGLLKL WKP 513 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0005634; C:nucleus; IEA:UniProtKB-SubCell. 
Interpro
 IPR006595; CTLH_C.
 IPR020472; G-protein_beta_WD-40_rep.
 IPR006594; LisH_dimerisation.
 IPR015943; WD40/YVTN_repeat-like_dom.
 IPR001680; WD40_repeat.
 IPR019775; WD40_repeat_CS.
 IPR017986; WD40_repeat_dom. 
Pfam
 PF00400; WD40 
SMART
 SM00668; CTLH
 SM00667; LisH
 SM00320; WD40 
PROSITE
 PS50897; CTLH
 PS50896; LISH
 PS00678; WD_REPEATS_1
 PS50082; WD_REPEATS_2
 PS50294; WD_REPEATS_REGION 
PRINTS
 PR00320; GPROTEINBRPT.