CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-011909
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Aspartyl/asparaginyl beta-hydroxylase 
Protein Synonyms/Alias
 Aspartate beta-hydroxylase; ASP beta-hydroxylase; Peptide-aspartate beta-dioxygenase 
Gene Name
 ASPH 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
8MAQRKNAKSSGNSSSubiquitination[1]
104DFDVDDAKVLLGLKEubiquitination[1]
110AKVLLGLKERSTSEPubiquitination[1, 2, 3]
512AESIPYLKEGIESGDubiquitination[1]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 
Functional Description
 Isoform 1: specifically hydroxylates an Asp or Asn residue in certain epidermal growth factor-like (EGF) domains of a number of proteins. 
Sequence Annotation
 REPEAT 341 374 TPR 1.
 REPEAT 454 487 TPR 2.
 REPEAT 489 521 TPR 3.
 REPEAT 525 557 TPR 4.
 CARBOHYD 452 452 N-linked (GlcNAc...).
 CARBOHYD 706 706 N-linked (GlcNAc...) (Potential).  
Keyword
 3D-structure; Alternative splicing; Calcium; Complete proteome; Dioxygenase; Endoplasmic reticulum; Glycoprotein; Iron; Membrane; Metal-binding; Oxidoreductase; Polymorphism; Reference proteome; Repeat; Signal-anchor; TPR repeat; Transmembrane; Transmembrane helix. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 758 AA 
Protein Sequence
MAQRKNAKSS GNSSSSGSGS GSTSAGSSSP GARRETKHGG HKNGRKGGLS GTSFFTWFMV 60
IALLGVWTSV AVVWFDLVDY EEVLGKLGIY DADGDGDFDV DDAKVLLGLK ERSTSEPAVP 120
PEEAEPHTEP EEQVPVEAEP QNIEDEAKEQ IQSLLHEMVH AEHVEGEDLQ QEDGPTGEPQ 180
QEDDEFLMAT DVDDRFETLE PEVSHEETEH SYHVEETVSQ DCNQDMEEMM SEQENPDSSE 240
PVVEDERLHH DTDDVTYQVY EEQAVYEPLE NEGIEITEVT APPEDNPVED SQVIVEEVSI 300
FPVEEQQEVP PETNRKTDDP EQKAKVKKKK PKLLNKFDKT IKAELDAAEK LRKRGKIEEA 360
VNAFKELVRK YPQSPRARYG KAQCEDDLAE KRRSNEVLRG AIETYQEVAS LPDVPADLLK 420
LSLKRRSDRQ QFLGHMRGSL LTLQRLVQLF PNDTSLKNDL GVGYLLIGDN DNAKKVYEEV 480
LSVTPNDGFA KVHYGFILKA QNKIAESIPY LKEGIESGDP GTDDGRFYFH LGDAMQRVGN 540
KEAYKWYELG HKRGHFASVW QRSLYNVNGL KAQPWWTPKE TGYTELVKSL ERNWKLIRDE 600
GLAVMDKAKG LFLPEDENLR EKGDWSQFTL WQQGRRNENA CKGAPKTCTL LEKFPETTGC 660
RRGQIKYSIM HPGTHVWPHT GPTNCRLRMH LGLVIPKEGC KIRCANETKT WEEGKVLIFD 720
DSFEHEVWQD ASSFRLIFIV DVWHPELTPQ QRRSLPAI 758 
Gene Ontology
 GO:0034704; C:calcium channel complex; TAS:BHF-UCL.
 GO:0032541; C:cortical endoplasmic reticulum; IDA:UniProtKB.
 GO:0005789; C:endoplasmic reticulum membrane; NAS:UniProtKB.
 GO:0030176; C:integral to endoplasmic reticulum membrane; IDA:UniProtKB.
 GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
 GO:0014701; C:junctional sarcoplasmic reticulum membrane; TAS:BHF-UCL.
 GO:0005886; C:plasma membrane; IDA:UniProtKB.
 GO:0033018; C:sarcoplasmic reticulum lumen; TAS:BHF-UCL.
 GO:0005509; F:calcium ion binding; IDA:UniProtKB.
 GO:0009055; F:electron carrier activity; TAS:UniProtKB.
 GO:0044325; F:ion channel binding; TAS:BHF-UCL.
 GO:0016702; F:oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen; IEA:UniProtKB-KW.
 GO:0004597; F:peptide-aspartate beta-dioxygenase activity; TAS:ProtInc.
 GO:0008307; F:structural constituent of muscle; TAS:ProtInc.
 GO:0097202; P:activation of cysteine-type endopeptidase activity; IDA:BHF-UCL.
 GO:0032237; P:activation of store-operated calcium channel activity; IDA:UniProtKB.
 GO:0070588; P:calcium ion transmembrane transport; IDA:UniProtKB.
 GO:0071277; P:cellular response to calcium ion; IDA:UniProtKB.
 GO:0005513; P:detection of calcium ion; TAS:BHF-UCL.
 GO:0060325; P:face morphogenesis; IEA:Compara.
 GO:0035108; P:limb morphogenesis; IEA:Compara.
 GO:0006936; P:muscle contraction; TAS:ProtInc.
 GO:0008285; P:negative regulation of cell proliferation; IEA:Compara.
 GO:0060021; P:palate development; IEA:Compara.
 GO:0007389; P:pattern specification process; IEA:Compara.
 GO:0042264; P:peptidyl-aspartic acid hydroxylation; IEA:Compara.
 GO:0010524; P:positive regulation of calcium ion transport into cytosol; IDA:UniProtKB.
 GO:0090316; P:positive regulation of intracellular protein transport; IDA:UniProtKB.
 GO:0045862; P:positive regulation of proteolysis; IDA:BHF-UCL.
 GO:0060316; P:positive regulation of ryanodine-sensitive calcium-release channel activity; TAS:BHF-UCL.
 GO:0045893; P:positive regulation of transcription, DNA-dependent; IMP:UniProtKB.
 GO:0010881; P:regulation of cardiac muscle contraction by regulation of the release of sequestered calcium ion; ISS:BHF-UCL.
 GO:0010649; P:regulation of cell communication by electrical coupling; TAS:BHF-UCL.
 GO:0031585; P:regulation of inositol 1,4,5-trisphosphate-sensitive calcium-release channel activity; IDA:UniProtKB.
 GO:0033198; P:response to ATP; IDA:UniProtKB. 
Interpro
 IPR007943; Asp-B-hydro/Triadin_dom.
 IPR007803; Asp_Arg_b-Hydrxlase.
 IPR027443; IPNS-like.
 IPR013026; TPR-contain_dom.
 IPR011990; TPR-like_helical.
 IPR019734; TPR_repeat. 
Pfam
 PF05279; Asp-B-Hydro_N
 PF05118; Asp_Arg_Hydrox 
SMART
  
PROSITE
 PS50005; TPR
 PS50293; TPR_REGION 
PRINTS