UniProt Accession

 KCRB_RAT; P07335; A0JPK7; Q499P7; Q5PPJ5; Q6IRE0; Q6P139 

Genbank Protein ID

 M14400; M18668; M57664; M57665; BC065307; BC070955; BC087656; BC099814; BC127477 

Genbank Nucleotide ID

 AAA40930.1; AAA40932.1; AAA40933.1; AAA40931.1; AAH65307.2; AAH70955.2; AAH87656.1; AAH99814.2; AAI27478.2 

Protein Name

 Creatine kinase B-type 

Protein Synonyms/Alias

 B-CK; Creatine kinase B chain 

Gene Name

 Ckb 

Gene Synonyms/Alias

 Ckbb 

Created Date

 July 27, 2013 

Organism

 Rattus norvegicus (Rat) 

NCBI Taxa ID

 10116 

Lysine Modification

Position	Peptide	Type	References
11	SNSHNTQKLRFPAED	acetylation	[1]
32	SHNNHMAKVLTPELY	acetylation	[1]
156	GERRAIEKLAVEALS	acetylation	[1]
196	DDHFLFDKPVSPLLL	acetylation	[1]
242	LRVISMQKGGNMKEV	acetylation	[1]
242	LRVISMQKGGNMKEV	ubiquitination	[2]
247	MQKGGNMKEVFTRFC	acetylation	[1]
265	TQIETLFKSKNYEFM	acetylation	[1]
298	LRAGVHIKLPHLGKH	acetylation	[1]
304	IKLPHLGKHEKFSEV	acetylation	[1]
304	IKLPHLGKHEKFSEV	ubiquitination	[2]
307	PHLGKHEKFSEVLKR	acetylation	[1]
313	EKFSEVLKRLRLQKR	acetylation	[1]
313	EKFSEVLKRLRLQKR	ubiquitination	[2]

Reference

 [1] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
 Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
 Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405]
 [2] Synaptic protein ubiquitination in rat brain revealed by antibody-based ubiquitome analysis.
 Na CH, Jones DR, Yang Y, Wang X, Xu Y, Peng J.
 J Proteome Res. 2012 Sep 7;11(9):4722-32. [PMID: 22871113] 

Functional Description

 Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa. 

Sequence Annotation

 DOMAIN 11 98 Phosphagen kinase N-terminal.
 DOMAIN 125 367 Phosphagen kinase C-terminal.
 NP_BIND 128 132 ATP (By similarity).
 NP_BIND 320 325 ATP (By similarity).
 BINDING 130 130 ATP (By similarity).
 BINDING 132 132 ATP (By similarity).
 BINDING 191 191 ATP (By similarity).
 BINDING 232 232 Substrate (By similarity).
 BINDING 236 236 ATP (By similarity).
 BINDING 285 285 Substrate (By similarity).
 BINDING 292 292 ATP (By similarity).
 BINDING 320 320 ATP (By similarity).
 BINDING 335 335 ATP (By similarity).
 MOD_RES 4 4 Phosphoserine (By similarity).
 MOD_RES 35 35 Phosphothreonine (By similarity).
 MOD_RES 39 39 Phosphotyrosine (By similarity).
 MOD_RES 125 125 Phosphotyrosine (By similarity).
 MOD_RES 164 164 Phosphoserine (By similarity).
 MOD_RES 199 199 Phosphoserine (By similarity).
 MOD_RES 269 269 Nitrated tyrosine (By similarity).  

Keyword

 ATP-binding; Complete proteome; Cytoplasm; Direct protein sequencing; Kinase; Nitration; Nucleotide-binding; Phosphoprotein; Reference proteome; Transferase. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 381 AA 

Protein Sequence

Gene Ontology

 GO:0005739; C:mitochondrion; IEA:Compara.
 GO:0005886; C:plasma membrane; TAS:RGD.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0004111; F:creatine kinase activity; TAS:RGD.
 GO:0007420; P:brain development; IEP:RGD.
 GO:0030644; P:cellular chloride ion homeostasis; IMP:RGD.
 GO:0006603; P:phosphocreatine metabolic process; TAS:RGD. 

Interpro

 IPR000749; ATP-guanido_PTrfase.
 IPR022415; ATP-guanido_PTrfase_AS.
 IPR022414; ATP-guanido_PTrfase_cat.
 IPR022413; ATP-guanido_PTrfase_N.
 IPR014746; Gln_synth/guanido_kin_cat_dom. 

Pfam

 PF00217; ATP-gua_Ptrans
 PF02807; ATP-gua_PtransN 

SMART

  

PROSITE

 PS00112; PHOSPHAGEN_KINASE
 PS51510; PHOSPHAGEN_KINASE_C
 PS51509; PHOSPHAGEN_KINASE_N 

PRINTS