CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-009624
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 60S ribosomal protein L8 
Protein Synonyms/Alias
  
Gene Name
 RPL8 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
10RVIRGQRKGAGSVFRubiquitination[1]
42AERHGYIKGIVKDIIubiquitination[1, 2, 3]
46GYIKGIVKDIIHDPGacetylation[4]
46GYIKGIVKDIIHDPGubiquitination[1, 2, 3, 5, 6, 7]
60GRGAPLAKVVFRDPYubiquitination[1, 2, 3, 5, 6, 7]
93QFVYCGKKAQLNIGNubiquitination[1]
119IVCCLEEKPGDRGKLubiquitination[1, 2, 6]
144ISHNPETKKTRVKLPubiquitination[1, 2, 6, 8]
145SHNPETKKTRVKLPSubiquitination[1]
155VKLPSGSKKVISSANubiquitination[6]
177AGGGRIDKPILKAGRubiquitination[1, 2]
181RIDKPILKAGRAYHKubiquitination[1, 2, 3, 5, 6, 7]
188KAGRAYHKYKAKRNCubiquitination[2]
234RDAPAGRKVGLIAARubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [4] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [5] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [6] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [7] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [8] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724] 
Functional Description
  
Sequence Annotation
 MOD_RES 216 216 (3S)-3-hydroxyhistidine.  
Keyword
 3D-structure; Complete proteome; Cytoplasm; Direct protein sequencing; Hydroxylation; Polymorphism; Reference proteome; Ribonucleoprotein; Ribosomal protein; RNA-binding; rRNA-binding. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 257 AA 
Protein Sequence
MGRVIRGQRK GAGSVFRAHV KHRKGAARLR AVDFAERHGY IKGIVKDIIH DPGRGAPLAK 60
VVFRDPYRFK KRTELFIAAE GIHTGQFVYC GKKAQLNIGN VLPVGTMPEG TIVCCLEEKP 120
GDRGKLARAS GNYATVISHN PETKKTRVKL PSGSKKVISS ANRAVVGVVA GGGRIDKPIL 180
KAGRAYHKYK AKRNCWPRVR GVAMNPVEHP FGGGNHQHIG KPSTIRRDAP AGRKVGLIAA 240
RRTGRLRGTK TVQEKEN 257 
Gene Ontology
 GO:0022625; C:cytosolic large ribosomal subunit; IDA:UniProtKB.
 GO:0003723; F:RNA binding; TAS:ProtInc.
 GO:0019843; F:rRNA binding; IEA:UniProtKB-KW.
 GO:0003735; F:structural constituent of ribosome; NAS:UniProtKB.
 GO:0000184; P:nuclear-transcribed mRNA catabolic process, nonsense-mediated decay; TAS:Reactome.
 GO:0006614; P:SRP-dependent cotranslational protein targeting to membrane; TAS:Reactome.
 GO:0006414; P:translational elongation; TAS:Reactome.
 GO:0006413; P:translational initiation; TAS:Reactome.
 GO:0006415; P:translational termination; TAS:Reactome.
 GO:0019083; P:viral transcription; TAS:Reactome. 
Interpro
 IPR012340; NA-bd_OB-fold.
 IPR022666; Rbsml_prot_L2_RNA-bd_dom.
 IPR014722; Rib_L2_dom2.
 IPR002171; Ribosomal_L2.
 IPR023672; Ribosomal_L2_arc.
 IPR022669; Ribosomal_L2_C.
 IPR022671; Ribosomal_L2_CS.
 IPR014726; Ribosomal_L2_dom3.
 IPR008991; Translation_prot_SH3-like. 
Pfam
 PF00181; Ribosomal_L2
 PF03947; Ribosomal_L2_C 
SMART
  
PROSITE
 PS00467; RIBOSOMAL_L2 
PRINTS