CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-008887
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 NEDD8 ultimate buster 1 
Protein Synonyms/Alias
 Negative regulator of ubiquitin-like proteins 1; Protein BS4 
Gene Name
 Nub1 
Gene Synonyms/Alias
 Nyren18 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
168QSEEDVRKNLQLEEEubiquitination[1]
183EQNEAELKERRIQRTubiquitination[1]
311KKLNLAQKCFKNCYGubiquitination[1]
314NLAQKCFKNCYGENHubiquitination[1]
328HQRLVHIKGNCGKEKubiquitination[1]
335KGNCGKEKVLFLRLYacetylation[2]
370NKARQLFKELYIDPSubiquitination[1]
449GYSTQAAKQALHQARubiquitination[1]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [2] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377] 
Functional Description
 Specific down-regulator of the NEDD8 conjugation system. Recruits NEDD8, UBD, and their conjugates to the proteasome for degradation (By similarity). 
Sequence Annotation
 DOMAIN 373 413 UBA 1.
 DOMAIN 423 469 UBA 2.
 DOMAIN 488 528 UBA 3.
 REGION 426 473 NEDD8-binding 1 (By similarity).
 REGION 549 597 NEDD8-binding 2 (By similarity).
 MOTIF 413 430 Nuclear localization signal (Probable).  
Keyword
 3D-structure; Coiled coil; Complete proteome; Nucleus; Reference proteome; Repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 614 AA 
Protein Sequence
MAQKKYLQAK LTQFLREDRI QLWKPPYTKE NKEVGLAVKD LAKKYSERLE CCENEVENII 60
EEIRRKAIER GTGNEHYRTT GIATIEVFLP PRLRKHDKKS LLETRLHVTG RDLRCQIAET 120
FGFQENYIKI VINKKQLQLG KSLEEQGVTH NVKAMVLELK QSEEDVRKNL QLEEEEQNEA 180
ELKERRIQRT KRGLEILAER AEMVVDPETM PYLDIANQTG RSLRIPPAER KALMLAMGYH 240
EKGRAFLKRK EYGIALPCLL DADRYFCECK ELLDTVDNYA VLQLDIVWCY FRLEQLECLD 300
DAEKKLNLAQ KCFKNCYGEN HQRLVHIKGN CGKEKVLFLR LYLLQGIQNY HSGNGEEARE 360
YLNKARQLFK ELYIDPSKVH NLLQLGFTAQ EARLGLRACD GNVDHAATHI SNRREELAQI 420
RKEEKEKRRR RLENVNTLRG MGYSTQAAKQ ALHQARGNLD DALKVLLSNP HMWWLQDADP 480
ENNSRQASPS QESINQLVYM GFDTVVAEAA LRVFGGNVQL AAQTLAHHGG SLPPDLQFSG 540
EDSSPTPSTS PSDSAGTSSA STDEDMETEA VNEILEDIPE HEEDYLDSTL EDEEVIIAEY 600
LSYVESISSA AKNN 614 
Gene Ontology
 GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
 GO:0032436; P:positive regulation of proteasomal ubiquitin-dependent protein catabolic process; ISS:UniProtKB.
 GO:0016567; P:protein ubiquitination; ISS:UniProtKB.
 GO:0034341; P:response to interferon-gamma; IEA:Compara.
 GO:0034612; P:response to tumor necrosis factor; IEA:Compara.
 GO:0006511; P:ubiquitin-dependent protein catabolic process; ISS:UniProtKB. 
Interpro
 IPR009060; UBA-like.
 IPR000449; UBA/transl_elong_EF1B_N.
 IPR015940; UBA/transl_elong_EF1B_N_euk. 
Pfam
 PF00627; UBA 
SMART
 SM00165; UBA 
PROSITE
 PS50030; UBA 
PRINTS