UniProt Accession

 IFM3_MOUSE; Q9CQW9; Q9D8L6 

Genbank Protein ID

 AY082484; AY594690; AK003407; AK007916; AK007919; AK151890; CH466531; BC010291 

Genbank Nucleotide ID

 AAM03316.1; AAT06089.1; BAB22771.1; BAB25347.1; BAB25350.1; BAE30774.1; EDL17977.1; AAH10291.1 

Protein Name

 Interferon-induced transmembrane protein 3 

Protein Synonyms/Alias

 Dispanin subfamily A member 2b; DSPA2b; Fragilis protein; Interferon-inducible protein 15; Mouse ifitm-like protein 1; Mil-1 

Gene Name

 Ifitm3 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
24	PPNYERIKEEYEVAE	acetylation	[1]
24	PPNYERIKEEYEVAE	ubiquitination	[2]
88	SVKSRDRKMVGDVTG	ubiquitination	[2]

Reference

 [1] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337]
 [2] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 

Functional Description

 IFN-induced antiviral protein which inhibits the entry of viruses to the host cell cytoplasm, permitting endocytosis, but preventing subsequent viral fusion and release of viral contents into the cytosol. Active against multiple viruses, including influenza A virus, SARS coronavirus (SARS-CoV), Marburg virus (MARV) and Ebola virus (EBOV), Dengue virus (DNV), West Nile virus (WNV) and human immunodeficiency virus type 1 (HIV-1). Can inhibit: influenza virus hemagglutinin protein-mediated viral entry, MARV and EBOV GP1,2-mediated viral entry and SARS-CoV S protein-mediated viral entry. Plays a critical role in the structural stability and function of vacuolar ATPase (v-ATPase). Establishes physical contact with the v-ATPase of endosomes which is critical for proper clathrin localization and is also required for the function of the v-ATPase to lower the pH in phagocytic endosomes thus establishing an antiviral state. Involved in initiating germ cell competence and specification, and in the demarcation of PGCs from their somatic neighbors. 

Sequence Annotation

 REGION 60 93 Interaction with SPP1 (By similarity).
 MOD_RES 27 27 Phosphotyrosine.
 LIPID 71 71 S-palmitoyl cysteine (By similarity).
 LIPID 72 72 S-palmitoyl cysteine (By similarity).
 LIPID 105 105 S-palmitoyl cysteine (By similarity).
 CROSSLNK 24 24 Glycyl lysine isopeptide (Lys-Gly)
 CROSSLNK 83 83 Glycyl lysine isopeptide (Lys-Gly)
 CROSSLNK 88 88 Glycyl lysine isopeptide (Lys-Gly)
 CROSSLNK 104 104 Glycyl lysine isopeptide (Lys-Gly)  

Keyword

 Antiviral defense; Cell membrane; Complete proteome; Endosome; Immunity; Innate immunity; Isopeptide bond; Lipoprotein; Lysosome; Membrane; Palmitate; Phosphoprotein; Reference proteome; Signal-anchor; Transmembrane; Transmembrane helix; Ubl conjugation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 137 AA 

Protein Sequence

Gene Ontology

 GO:0016023; C:cytoplasmic membrane-bounded vesicle; IDA:MGI.
 GO:0005783; C:endoplasmic reticulum; IDA:MGI.
 GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
 GO:0031902; C:late endosome membrane; IEA:UniProtKB-SubCell.
 GO:0005765; C:lysosomal membrane; IEA:UniProtKB-SubCell.
 GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
 GO:0051607; P:defense response to virus; IMP:MGI.
 GO:0008285; P:negative regulation of cell proliferation; IDA:MGI.
 GO:0046597; P:negative regulation of viral entry into host cell; IDA:UniProtKB.
 GO:0060337; P:type I interferon-mediated signaling pathway; IDA:MGI. 

Interpro

 IPR007593; Interferon-induced_TM_protein. 

Pfam

 PF04505; CD225 

SMART

  

PROSITE

  

PRINTS