CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-023886
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 BTB/POZ domain-containing protein KCTD3 
Protein Synonyms/Alias
 Renal carcinoma antigen NY-REN-45 
Gene Name
 KCTD3 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
81ILNFLRTKELDLRGVubiquitination[1]
237ERVALNAKVVGGPHGubiquitination[2]
350QKFPLRMKDNDLLVTubiquitination[2]
426VHRSPVTKIMLSEKHubiquitination[3, 4]
503DQQVFIQKVVPITNKubiquitination[2, 4, 5]
510KVVPITNKLFVRLSSubiquitination[1, 4, 5]
796TASPSPTKTTPSPRHphosphoglycerylation[6]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [4] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [5] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [6] Functional lysine modification by an intrinsically reactive primary glycolytic metabolite.
 Moellering RE, Cravatt BF.
 Science. 2013 Aug 2;341(6145):549-53. [PMID: 23908237
Functional Description
  
Sequence Annotation
 DOMAIN 18 87 BTB.
 REPEAT 224 271 WD 1.
 REPEAT 276 313 WD 2.
 REPEAT 315 350 WD 3.
 REPEAT 419 457 WD 4.
 REPEAT 537 577 WD 5.  
Keyword
 Alternative splicing; Complete proteome; Polymorphism; Reference proteome; Repeat; WD repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 815 AA 
Protein Sequence
MAGGHCGSFP AAAAGSGEIV QLNVGGTRFS TSRQTLMWIP DSFFSSLLSG RISTLRDETG 60
AIFIDRDPAA FAPILNFLRT KELDLRGVSI NVLRHEAEFY GITPLVRRLL LCEELERSSC 120
GSVLFHGYLP PPGIPSRKIN NTVRSADSRN GLNSTEGEAR GNGTQPVLSG TGEETVRLGF 180
PVDPRKVLIV AGHHNWIVAA YAHFAVCYRI KESSGWQQVF TSPYLDWTIE RVALNAKVVG 240
GPHGDKDKMV AVASESSIIL WSVQDGGSGS EIGVFSLGVP VDALFFIGNQ LVATSHTGKV 300
GVWNAVTQHW QVQDVVPITS YDTAGSFLLL GCNNGSIYYI DMQKFPLRMK DNDLLVTELY 360
HDPSNDAITA LSVYLTPKTS VSGNWIEIAY GTSSGAVRVI VQHPETVGSG PQLFQTFTVH 420
RSPVTKIMLS EKHLVSVCAD NNHVRTWTVT RFRGMISTQP GSTPLASFKI LSLEETESHG 480
SYSSGNDIGP FGERDDQQVF IQKVVPITNK LFVRLSSTGK RICEIQAVDC TTISSFTVRE 540
CEGSSRMGSR PRRYLFTGHT NGSIQMWDLT TAMDMVNKSE DKDVGGPTEE ELLKLLDQCD 600
LSTSRCATPN ISPATSVVQH SHLRESNSSL QLQHHDTTHE AATYGSMRPY RESPLLARAR 660
RTESFHSYRD FQTINLNRNV ERAVPENGNL GPIQAEVKGA TGECNISERK SPGVEIKSLR 720
ELDSGLEVHK IAEGFSESKK RSSEDENENK IEFRKKGGFE GGGFLGRKKV PYLASSPSTS 780
DGGTDSPGTA SPSPTKTTPS PRHKKSDSSG QEYSL 815 
Gene Ontology
 GO:0051260; P:protein homooligomerization; IEA:InterPro. 
Interpro
 IPR000210; BTB/POZ-like.
 IPR011333; BTB/POZ_fold.
 IPR003131; T1-type_BTB.
 IPR015943; WD40/YVTN_repeat-like_dom.
 IPR001680; WD40_repeat.
 IPR019775; WD40_repeat_CS.
 IPR017986; WD40_repeat_dom. 
Pfam
 PF02214; K_tetra 
SMART
 SM00225; BTB
 SM00320; WD40 
PROSITE
 PS50097; BTB
 PS00678; WD_REPEATS_1
 PS50082; WD_REPEATS_2
 PS50294; WD_REPEATS_REGION 
PRINTS