| Tag | Content |
|---|
| CPLM ID | CPLM-015624 |
UniProt Accession | |
Genbank Protein ID | |
Genbank Nucleotide ID | |
Protein Name | Phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 2 protein |
Protein Synonyms/Alias | P-Rex2; PtdIns(3,4,5)-dependent Rac exchanger 2; DEP domain-containing protein 2 |
Gene Name | PREX2 |
Gene Synonyms/Alias | DEPDC2 |
Created Date | July 27, 2013 |
Organism | Homo sapiens (Human) |
NCBI Taxa ID | 9606 |
Lysine Modification | Position | Peptide | Type | References |
|---|
| 138 | KLLLELNKIRTIRTF | ubiquitination | [1] | | 592 | VVENVIAKSLLIKSN | ubiquitination | [1, 2] | | 673 | LRVLVSTKPRETVKI | ubiquitination | [1] | | 679 | TKPRETVKIPDSADG | ubiquitination | [1] |
|
Reference | [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments. Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA. Mol Cell Proteomics. 2013 Mar;12(3):825-31. [ PMID: 23266961] [2] Integrated proteomic analysis of post-translational modifications by serial enrichment. Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA. Nat Methods. 2013 Jul;10(7):634-7. [ PMID: 23749302] |
Functional Description | Functions as a RAC1 guanine nucleotide exchange factor (GEF), activating Rac proteins by exchanging bound GDP for free GTP. Its activity is synergistically activated by phosphatidylinositol 3,4,5-trisphosphate and the beta gamma subunits of heterotrimeric G protein. Mediates the activation of RAC1 in a PI3K-dependent manner. May be an important mediator of Rac signaling, acting directly downstream of both G protein- coupled receptors and phosphoinositide 3-kinase. |
Sequence Annotation | DOMAIN 23 214 DH.
DOMAIN 245 361 PH.
DOMAIN 390 464 DEP 1.
DOMAIN 491 566 DEP 2.
DOMAIN 592 671 PDZ 1.
DOMAIN 677 754 PDZ 2. |
Keyword | Alternative splicing; Complete proteome; Guanine-nucleotide releasing factor; Polymorphism; Reference proteome; Repeat. |
Sequence Source | UniProt (SWISSPROT/TrEMBL); GenBank; EMBL |
Protein Length | 1606 AA |
Protein Sequence | MSEDSRGDSR AESAKDLEKQ LRLRVCVLSE LQKTERDYVG TLEFLVSAFL HRMNQCAASK 60
VDKNVTEETV KMLFSNIEDI LAVHKEFLKV VEECLHPEPN AQQEVGTCFL HFKDKFRIYD 120
EYCSNHEKAQ KLLLELNKIR TIRTFLLNCM LLGGRKNTDV PLEGYLVTPI QRICKYPLIL 180
KELLKRTPRK HSDYAAVMEA LQAMKAVCSN INEAKRQMEK LEVLEEWQSH IEGWEGSNIT 240
DTCTEMLMCG VLLKISSGNI QERVFFLFDN LLVYCKRKHR RLKNSKASTD GHRYLFRGRI 300
NTEVMEVENV DDGTADFHSS GHIVVNGWKI HNTAKNKWFV CMAKTPEEKH EWFEAILKER 360
ERRKGLKLGM EQDTWVMISE QGEKLYKMMC RQGNLIKDRK RKLTTFPKCF LGSEFVSWLL 420
EIGEIHRPEE GVHLGQALLE NGIIHHVTDK HQFKPEQMLY RFRYDDGTFY PRNEMQDVIS 480
KGVRLYCRLH SLFTPVIRDK DYHLRTYKSV VMANKLIDWL IAQGDCRTRE EAMIFGVGLC 540
DNGFMHHVLE KSEFKDEPLL FRFFSDEEME GSNMKHRLMK HDLKVVENVI AKSLLIKSNE 600
GSYGFGLEDK NKVPIIKLVE KGSNAEMAGM EVGKKIFAIN GDLVFMRPFN EVDCFLKSCL 660
NSRKPLRVLV STKPRETVKI PDSADGLGFQ IRGFGPSVVH AVGRGTVAAA AGLHPGQCII 720
KVNGINVSKE THASVIAHVT ACRKYRRPTK QDSIQWVYNS IESAQEDLQK SHSKPPGDEA 780
GDAFDCKVEE VIDKFNTMAI IDGKKEHVSL TVDNVHLEYG VVYEYDSTAG IKCNVVEKMI 840
EPKGFFSLTA KILEALAKSD EHFVQNCTSL NSLNEVIPTD LQSKFSALCS ERIEHLCQRI 900
SSYKKFSRVL KNRAWPTFKQ AKSKISPLHS SDFCPTNCHV NVMEVSYPKT STSLGSAFGV 960
QLDSRKHNSH DKENKSSEQG KLSPMVYIQH TITTMAAPSG LSLGQQDGHG LRYLLKEEDL 1020
ETQDIYQKLL GKLQTALKEV EMCVCQIDDL LSSITYSPKL ERKTSEGIIP TDSDNEKGER 1080
NSKRVCFNVA GDEQEDSGHD TISNRDSYSD CNSNRNSIAS FTSICSSQCS SYFHSDEMDS 1140
GDELPLSVRI SHDKQDKIHS CLEHLFSQVD SITNLLKGQA VVRAFDQTKY LTPGRGLQEF 1200
QQEMEPKLSC PKRLRLHIKQ DPWNLPSSVR TLAQNIRKFV EEVKCRLLLA LLEYSDSETQ 1260
LRRDMVFCQT LVATVCAFSE QLMAALNQMF DNSKENEMET WEASRRWLDQ IANAGVLFHF 1320
QSLLSPNLTD EQAMLEDTLV ALFDLEKVSF YFKPSEEEPL VANVPLTYQA EGSRQALKVY 1380
FYIDSYHFEQ LPQRLKNGGG FKIHPVLFAQ ALESMEGYYY RDNVSVEEFQ AQINAASLEK 1440
VKQYNQKLRA FYLDKSNSPP NSTSKAAYVD KLMRPLNALD ELYRLVASFI RSKRTAACAN 1500
TACSASGVGL LSVSSELCNR LGACHIIMCS SGVHRCTLSV TLEQAIILAR SHGLPPRYIM 1560
QATDVMRKQG ARVQNTAKNL GVRDRTPQSA PRLYKLCEPP PPAGEE 1606 |
Gene Ontology | GO:0005622; C:intracellular; IEA:InterPro. GO:0005543; F:phospholipid binding; IEA:InterPro. GO:0030675; F:Rac GTPase activator activity; IDA:MGI. GO:0030676; F:Rac guanyl-nucleotide exchange factor activity; IDA:MGI. GO:0007186; P:G-protein coupled receptor signaling pathway; IDA:MGI. GO:0035556; P:intracellular signal transduction; IEA:InterPro. |
Interpro | |
Pfam | |
SMART | |
PROSITE | |
PRINTS | |