CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-014655
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Kynurenine/alpha-aminoadipate aminotransferase, mitochondrial 
Protein Synonyms/Alias
 KAT/AadAT; 2-aminoadipate aminotransferase; 2-aminoadipate transaminase; Alpha-aminoadipate aminotransferase; AadAT; Kynurenine aminotransferase II; Kynurenine--oxoglutarate aminotransferase II; Kynurenine--oxoglutarate transaminase 2; Kynurenine--oxoglutarate transaminase II 
Gene Name
 Aadat 
Gene Synonyms/Alias
 Kat2 
Created Date
 July 27, 2013 
Organism
 Rattus norvegicus (Rat) 
NCBI Taxa ID
 10116 
Lysine Modification
Position
Peptide
Type
References
31EIMSRAPKDIISLAPacetylation[1]
44APGSPNPKVFPFKSAacetylation[1]
49NPKVFPFKSAVFTVEacetylation[1]
179KKVLSQWKPEDSKDPacetylation[1]
184QWKPEDSKDPTKRTPacetylation[1]
192DPTKRTPKFLYTIPNacetylation[1]
213NSLTGDRKKEIYELAacetylation[1]
214SLTGDRKKEIYELARacetylation[1]
263IRADSLSKVISSGLRacetylation[1]
278VGFITGPKSLIQRIVacetylation[1]
327DRAIDFYKNQRDFILacetylation[1]
339FILAAADKWLRGLAEacetylation[1]
351LAEWHVPKAGMFLWIacetylation[1]
367VNGISDAKKLIEEKAacetylation[1]
373AKKLIEEKAIEREILacetylation[1]
422QRLAQLIKDVS****acetylation[1]
Reference
 [1] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
 Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
 Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405] 
Functional Description
 Transaminase with broad substrate specificity. Has transaminase activity towards aminoadipate, kynurenine, methionine and glutamate. Shows activity also towards tryptophan, aspartate and hydroxykynurenine. Accepts a variety of oxo-acids as amino- group acceptors, with a preference for 2-oxoglutarate, 2- oxocaproic acid, phenylpyruvate and alpha-oxo-gamma-methiol butyric acid. Can also use glyoxylate as amino-group acceptor (in vitro) (By similarity). 
Sequence Annotation
 BINDING 20 20 Substrate (By similarity).
 BINDING 74 74 Substrate (By similarity).
 BINDING 142 142 Substrate (By similarity).
 BINDING 202 202 Substrate (By similarity).
 BINDING 399 399 Substrate (By similarity).
 MOD_RES 263 263 N6-(pyridoxal phosphate)lysine (By  
Keyword
 Aminotransferase; Complete proteome; Direct protein sequencing; Mitochondrion; Pyridoxal phosphate; Reference proteome; Transferase; Transit peptide. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 425 AA 
Protein Sequence
MNYSRFLTAT SLARKTSPIR ATVEIMSRAP KDIISLAPGS PNPKVFPFKS AVFTVENGST 60
IRFEGEMFQR ALQYSSSYGI PELLSWLKQL QIKLHNPPTV NYSPNEGQMD LCITSGCQDG 120
LCKVFEMLIN PGDTVLVNEP LYSGALFAMK PLGCNFISVP SDDCGIIPEG LKKVLSQWKP 180
EDSKDPTKRT PKFLYTIPNG NNPTGNSLTG DRKKEIYELA RKYDFLIIED DPYYFLQFTK 240
PWEPTFLSMD VDGRVIRADS LSKVISSGLR VGFITGPKSL IQRIVLHTQI SSLHPCTLSQ 300
LMISELLYQW GEEGFLAHVD RAIDFYKNQR DFILAAADKW LRGLAEWHVP KAGMFLWIKV 360
NGISDAKKLI EEKAIEREIL LVPGNSFFVD NSAPSSFFRA SFSQVTPAQM DLVFQRLAQL 420
IKDVS 425 
Gene Ontology
 GO:0005739; C:mitochondrion; IDA:RGD.
 GO:0047536; F:2-aminoadipate transaminase activity; ISS:UniProtKB.
 GO:0016212; F:kynurenine-oxoglutarate transaminase activity; ISS:UniProtKB.
 GO:0030170; F:pyridoxal phosphate binding; IMP:RGD.
 GO:0006103; P:2-oxoglutarate metabolic process; ISS:UniProtKB.
 GO:0009058; P:biosynthetic process; IEA:InterPro.
 GO:0006536; P:glutamate metabolic process; ISS:UniProtKB.
 GO:0033512; P:L-lysine catabolic process to acetyl-CoA via saccharopine; IEA:UniProtKB-UniPathway.
 GO:0019441; P:tryptophan catabolic process to kynurenine; IDA:RGD. 
Interpro
 IPR004839; Aminotransferase_I/II.
 IPR015424; PyrdxlP-dep_Trfase.
 IPR015421; PyrdxlP-dep_Trfase_major_sub1.
 IPR015422; PyrdxlP-dep_Trfase_major_sub2. 
Pfam
 PF00155; Aminotran_1_2 
SMART
  
PROSITE
  
PRINTS