UniProt Accession

 RIMKB_HUMAN; Q9ULI2; B7Z834; D3DUV2; Q8N4P4; Q8WTW6 

Genbank Protein ID

 AB033064; AK302847; CH471116; CH471116; BC021977; BC033793 

Genbank Nucleotide ID

 BAA86552.1; BAH13820.1; EAW88610.1; EAW88611.1; AAH21977.2; AAH33793.1 

Protein Name

 Beta-citryl-glutamate synthase B 

Protein Synonyms/Alias

 N-acetyl-aspartyl-glutamate synthetase B; NAAG synthetase B; NAAGS; Ribosomal protein S6 modification-like protein B 

Gene Name

 RIMKLB 

Gene Synonyms/Alias

 FAM80B; KIAA1238 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
24	IREDYPQKEILRALK	ubiquitination	[1]
33	ILRALKAKCCEEELD	ubiquitination	[1]
158	LEFPMVVKNTRGHRG	ubiquitination	[2]
174	AVFLARDKHHLADLS	ubiquitination	[2]
194	EAPYLFQKYVKESHG	ubiquitination	[1, 2, 3]
197	YLFQKYVKESHGRDV	ubiquitination	[3]
365	TERELLTKLPGGLFN	ubiquitination	[1, 2]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983] 

Functional Description

 Catalyzes the synthesis of beta-citryl-glutamate and N- acetyl-aspartyl-glutamate. Beta-citryl-glutamate is synthesized more efficiently than N-acetyl-aspartyl-glutamate (By similarity). 

Sequence Annotation

 DOMAIN 119 304 ATP-grasp.
 NP_BIND 193 203 ATP (By similarity).
 METAL 264 264 Magnesium or manganese 1 (By similarity).
 METAL 277 277 Magnesium or manganese 1 (By similarity).
 METAL 277 277 Magnesium or manganese 2 (By similarity).
 METAL 279 279 Magnesium or manganese 2 (By similarity).
 BINDING 158 158 ATP (By similarity).
 BINDING 219 219 ATP (By similarity).  

Keyword

 Alternative splicing; ATP-binding; Complete proteome; Cytoplasm; Ligase; Magnesium; Manganese; Metal-binding; Nucleotide-binding; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 386 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; ISS:UniProtKB.
 GO:0016881; F:acid-amino acid ligase activity; IEA:EC.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0072591; F:citrate-L-glutamate ligase activity; ISS:UniProtKB.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0072590; F:N-acetyl-L-aspartate-L-glutamate ligase activity; ISS:UniProtKB.
 GO:0006464; P:cellular protein modification process; IEA:InterPro. 

Interpro

 IPR011761; ATP-grasp.
 IPR013651; ATP-grasp_RimK-type.
 IPR013815; ATP_grasp_subdomain_1.
 IPR013816; ATP_grasp_subdomain_2.
 IPR016185; PreATP-grasp_dom.
 IPR004666; RpS6_RimK/Lys_biosynth_LsyX. 

Pfam

 PF08443; RimK 

SMART

  

PROSITE

 PS50975; ATP_GRASP 

PRINTS