CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-037638
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 Ribosomal protein S6 kinase 
Protein Synonyms/Alias
  
Gene Name
 RPS6KA4 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
37VENFELLKVLGTGAYubiquitination[1, 2, 3]
134LYQRQYFKEAEVRVYubiquitination[3]
191KEFLTEEKERTFSFCubiquitination[2]
279LLQRLLCKDPKKRLGubiquitination[3]
313WVALAARKIPAPFRPubiquitination[3]
433SGQEFAVKILSRRLEubiquitination[1]
525GVVHRDLKPENILYAubiquitination[2, 3]
541DTPGAPVKIIDFGFAubiquitination[2]
654LLTVDPAKRLKLEGLubiquitination[3]
657VDPAKRLKLEGLRGSubiquitination[3]
713KREGFFLKSVENAPLubiquitination[3]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 
Functional Description
  
Sequence Annotation
  
Keyword
 ATP-binding; Complete proteome; Kinase; Nucleotide-binding; Reference proteome; Serine/threonine-protein kinase; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 765 AA 
Protein Sequence
MGDEDDDESC AVELRITEAN LTGHEEKVSV ENFELLKVLG TGAYGKVFLV RKAGGHDAGK 60
LYAMKVLRKA ALVQRAKTQE HTRTERSVLE LVRQAPFLVT LHYAFQTDAK LHLILDYVSG 120
GEMFTHLYQR QYFKEAEVRV YGGEIVLALE HLHKLGIIYR DLKLENVLLD SEGHIVLTDF 180
GLSKEFLTEE KERTFSFCGT IEYMAPEIIR SKTGHGKAVD WWSLGILLFE LLTGASPFTL 240
EGERNTQAEV SRRILKCSPP FPPRIGPVAQ DLLQRLLCKD PKKRLGAGPQ GAQEVRNHPF 300
FQGLDWVALA ARKIPAPFRP QIRSELDVGN FAEEFTRLEP VYSPPGSPPP GDPRIFQGYS 360
FVAPSILFDH NNAVMTDGLE APGAGDRPGR AAVARSAMMQ YELDLREPAL GQGSFSVCRR 420
CRQRQSGQEF AVKILSRRLE ANTQREVAAL RLCQSHPNVV NLHEVHHDQL HTYLVLELLR 480
GGELLEHIRK KRHFSESEAS QILRSLVSAV SFMHEEAGVV HRDLKPENIL YADDTPGAPV 540
KIIDFGFARL RPQSPGVPMQ TPCFTLQYAA PELLAQQGYD ESCDLWSLGV ILYMMLSGQV 600
PFQGASGQGG QSQAAEIMCK IREGRFSLDG EAWQGVSEEA KELVRGLLTV DPAKRLKLEG 660
LRGSSWLQDG SARSSPPLRT PDVLESSGPA VRSGLNATFM AFNRGKREGF FLKSVENAPL 720
AKRRKQKLRS ATASRRGSPA PANPGRAPVA SKGAPRRANG PLPPS 765 
Gene Ontology
 GO:0005737; C:cytoplasm; IDA:HPA.
 GO:0005634; C:nucleus; IDA:HPA.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0000287; F:magnesium ion binding; IEA:InterPro.
 GO:0004674; F:protein serine/threonine kinase activity; IEA:UniProtKB-KW.
 GO:0007243; P:intracellular protein kinase cascade; IEA:InterPro. 
Interpro
 IPR000961; AGC-kinase_C.
 IPR011009; Kinase-like_dom.
 IPR017892; Pkinase_C.
 IPR000719; Prot_kinase_cat_dom.
 IPR017441; Protein_kinase_ATP_BS.
 IPR016239; Ribosomal_S6_kinase_II.
 IPR002290; Ser/Thr_dual-sp_kinase_dom.
 IPR008271; Ser/Thr_kinase_AS. 
Pfam
 PF00069; Pkinase
 PF00433; Pkinase_C 
SMART
 SM00133; S_TK_X
 SM00220; S_TKc 
PROSITE
 PS51285; AGC_KINASE_CTER
 PS00107; PROTEIN_KINASE_ATP
 PS50011; PROTEIN_KINASE_DOM
 PS00108; PROTEIN_KINASE_ST 
PRINTS