CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-000843
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Phosphatidylinositol-binding clathrin assembly protein 
Protein Synonyms/Alias
 Clathrin assembly lymphoid myeloid leukemia protein; rCALM 
Gene Name
 Picalm 
Gene Synonyms/Alias
 Calm 
Created Date
 July 27, 2013 
Organism
 Rattus norvegicus (Rat) 
NCBI Taxa ID
 10116 
Lysine Modification
Position
Peptide
Type
References
134YSRYLNEKAVSYRQVubiquitination[1]
163MRTMNTEKLLKTVPIubiquitination[1]
318STGLSLTKVDEREKQubiquitination[1]
324TKVDEREKQAALEEEubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 
Functional Description
 Assembly protein recruiting clathrin and adapter protein complex 2 (AP2) to cell membranes at sites of coated-pit formation and clathrin-vesicle assembly. May be required to determine the amount of membrane to be recycled, possibly by regulating the size of the clathrin cage. Involved in AP2-dependent clathrin-mediated endocytosis at the neuromuscular junction (By similarity). 
Sequence Annotation
 DOMAIN 14 145 ENTH.
 REGION 221 294 Interaction with FAM64A (By similarity).
 MOD_RES 2 2 N-acetylserine (By similarity).
 MOD_RES 436 436 Phosphoserine (By similarity).  
Keyword
 3D-structure; Acetylation; Alternative splicing; Coated pit; Complete proteome; Cytoplasmic vesicle; Endocytosis; Golgi apparatus; Membrane; Nucleus; Phosphoprotein; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 640 AA 
Protein Sequence
MSGQSLTDRI TAAQHSVTGS AVSKTVCKAT THEIMGPKKK HLDYLIQCTN EMNVNIPQLA 60
DSLFERTTNS SWVVVFKSLI TTHHLMVYGN ERFIQYLASR NTLFNLSNFL DKSGLQGYDM 120
STFIRRYSRY LNEKAVSYRQ VAFDFTKVKR GADGVMRTMN TEKLLKTVPI IQNQMDALLD 180
FNVNSNELTN GVINAAFMLL FKDAIRLFAA YNEGIINLLE KYFDMKKNQC KEGLDIYKKF 240
LTRMTRISEF LKVAEQVGID RGDIPDLSQA PSSLLDALEQ HLASLEGKKI KDSTAASRAT 300
TLSNAVSSLA STGLSLTKVD EREKQAALEE EQARLKALKE QRLKELAKKP HTSLTTAASP 360
VSTSAGGIMT APAIDIFSTP SSSNSTSKLP NDLLDLQQPT FHPSVHAMSA APQVASTWGD 420
AVDDAIPSLN PFLTKSSGDV HLPISSDVST FTTRTPTHEM FVGFSPSPVT QPHPSAGLNV 480
DFESVFGNKS TNVAVDSGGG LLKPTVASQN QSLPVAKLPP NKLVSDDLDS SLANLVGNLG 540
IGNGTTKNDV SCSQPGEKKL TGGSNWQPKV APTTAWSAAT MAPPVMAYPA TTPTGMIGYG 600
IPPQMGSVPV MTQPTLIYSQ PVMRPPNPFG PVPGAQIQFM 640 
Gene Ontology
 GO:0030118; C:clathrin coat; IEA:InterPro.
 GO:0005905; C:coated pit; ISS:UniProtKB.
 GO:0005768; C:endosome; IDA:BHF-UCL.
 GO:0005794; C:Golgi apparatus; IEA:UniProtKB-SubCell.
 GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
 GO:0005886; C:plasma membrane; IDA:BHF-UCL.
 GO:0014069; C:postsynaptic density; IDA:BHF-UCL.
 GO:0045211; C:postsynaptic membrane; IDA:BHF-UCL.
 GO:0042734; C:presynaptic membrane; IDA:BHF-UCL.
 GO:0008021; C:synaptic vesicle; IDA:BHF-UCL.
 GO:0005545; F:1-phosphatidylinositol binding; IDA:UniProtKB.
 GO:0030276; F:clathrin binding; IDA:UniProtKB.
 GO:0017124; F:SH3 domain binding; IDA:RGD.
 GO:0007409; P:axonogenesis; IMP:BHF-UCL.
 GO:0048268; P:clathrin coat assembly; IDA:RGD.
 GO:0048813; P:dendrite morphogenesis; IMP:BHF-UCL.
 GO:0006898; P:receptor-mediated endocytosis; ISS:UniProtKB.
 GO:0030100; P:regulation of endocytosis; IMP:BHF-UCL.
 GO:0051223; P:regulation of protein transport; IMP:BHF-UCL. 
Interpro
 IPR011417; ANTH_dom.
 IPR014712; Clathrin_Pinositid-bd_GAT-like.
 IPR008942; ENTH_VHS.
 IPR013809; Epsin-like_N. 
Pfam
 PF07651; ANTH 
SMART
 SM00273; ENTH 
PROSITE
 PS50942; ENTH 
PRINTS