UniProt Accession

 CH10_MOUSE; Q64433 

Genbank Protein ID

 U09659; AF251024; AK088121; BC024385 

Genbank Nucleotide ID

 AAA62229.1; AAF67345.1; BAC40159.1; AAH24385.1 

Protein Name

 10 kDa heat shock protein, mitochondrial 

Protein Synonyms/Alias

 Hsp10; 10 kDa chaperonin; Chaperonin 10; CPN10 

Gene Name

 Hspe1 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
8	MAGQAFRKFLPLFDR	acetylation	[1, 2, 3, 4]
8	MAGQAFRKFLPLFDR	succinylation	[4]
8	MAGQAFRKFLPLFDR	ubiquitination	[5]
28	SAAETVTKGGIMLPE	acetylation	[2, 4, 6]
28	SAAETVTKGGIMLPE	succinylation	[4]
28	SAAETVTKGGIMLPE	ubiquitination	[5]
36	GGIMLPEKSQGKVLQ	acetylation	[4, 6, 7]
36	GGIMLPEKSQGKVLQ	succinylation	[4]
36	GGIMLPEKSQGKVLQ	ubiquitination	[5]
40	LPEKSQGKVLQATVV	acetylation	[2, 3, 4, 6, 7, 8, 9]
40	LPEKSQGKVLQATVV	succinylation	[4]
40	LPEKSQGKVLQATVV	ubiquitination	[5]
54	VAVGSGGKGKSGEIE	acetylation	[4]
54	VAVGSGGKGKSGEIE	succinylation	[4]
56	VGSGGKGKSGEIEPV	acetylation	[3, 4, 6, 7]
56	VGSGGKGKSGEIEPV	succinylation	[4]
56	VGSGGKGKSGEIEPV	succinylation	[4]
66	EIEPVSVKVGDKVLL	acetylation	[1, 2, 3, 4, 6, 7, 8, 9]
66	EIEPVSVKVGDKVLL	succinylation	[4]
66	EIEPVSVKVGDKVLL	succinylation	[4]
70	VSVKVGDKVLLPEYG	acetylation	[1, 2, 3, 4, 6, 7, 9]
70	VSVKVGDKVLLPEYG	succinylation	[4]
80	LPEYGGTKVVLDDKD	acetylation	[2, 3, 4, 6, 7, 9]
80	LPEYGGTKVVLDDKD	succinylation	[4]
86	TKVVLDDKDYFLFRD	acetylation	[1, 2, 3, 4, 6, 7, 8, 9, 10]
86	TKVVLDDKDYFLFRD	succinylation	[4]
86	TKVVLDDKDYFLFRD	ubiquitination	[5]
99	RDSDILGKYVD****	acetylation	[1, 2, 3, 6, 8, 10]

Reference

 [1] The fasted/fed mouse metabolic acetylome: N6-acetylation differences suggest acetylation coordinates organ-specific fuel switching.
 Yang L, Vaitheesvaran B, Hartil K, Robinson AJ, Hoopmann MR, Eng JK, Kurland IJ, Bruce JE.
 J Proteome Res. 2011 Sep 2;10(9):4134-49. [PMID: 21728379]
 [2] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
 [3] Label-free quantitative proteomics of the lysine acetylome in mitochondria identifies substrates of SIRT3 in metabolic pathways.
 Rardin MJ, Newman JC, Held JM, Cusack MP, Sorensen DJ, Li B, Schilling B, Mooney SD, Kahn CR, Verdin E, Gibson BW.
 Proc Natl Acad Sci U S A. 2013 Apr 16;110(16):6601-6. [PMID: 23576753]
 [4] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337]
 [5] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [6] Quantification of mitochondrial acetylation dynamics highlights prominent sites of metabolic regulation.
 Still AJ, Floyd BJ, Hebert AS, Bingman CA, Carson JJ, Gunderson DR, Dolan BK, Grimsrud PA, Dittenhafer-Reed KE, Stapleton DS, Keller MP, Westphall MS, Denu JM, Attie AD, Coon JJ, Pagliarini DJ.
 J Biol Chem. 2013 Jul 17;. [PMID: 23864654]
 [7] Calorie restriction and SIRT3 trigger global reprogramming of the mitochondrial protein acetylome.
 Hebert AS, Dittenhafer-Reed KE, Yu W, Bailey DJ, Selen ES, Boersma MD, Carson JJ, Tonelli M, Balloon AJ, Higbee AJ, Westphall MS, Pagliarini DJ, Prolla TA, Assadi-Porter F, Roy S, Denu JM, Coon JJ.
 Mol Cell. 2013 Jan 10;49(1):186-99. [PMID: 23201123]
 [8] Quantitative assessment of the impact of the gut microbiota on lysine epsilon-acetylation of host proteins using gnotobiotic mice.
 Simon GM, Cheng J, Gordon JI.
 Proc Natl Acad Sci U S A. 2012 Jul 10;109(28):11133-8. [PMID: 22733758]
 [9] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [10] Mitochondrial acetylome analysis in a mouse model of alcohol-induced liver injury utilizing SIRT3 knockout mice.
 Fritz KS, Galligan JJ, Hirschey MD, Verdin E, Petersen DR.
 J Proteome Res. 2012 Mar 2;11(3):1633-43. [PMID: 22309199] 

Functional Description

 Eukaryotic CPN10 homolog which is essential for mitochondrial protein biogenesis, together with CPN60. Binds to CPN60 in the presence of Mg-ATP and suppresses the ATPase activity of the latter. 

Sequence Annotation

 MOD_RES 2 2 N-acetylalanine (By similarity).
 MOD_RES 40 40 N6-malonyllysine (By similarity).
 MOD_RES 54 54 N6-malonyllysine (By similarity).
 MOD_RES 56 56 N6-acetyllysine; alternate (By
 MOD_RES 56 56 N6-malonyllysine; alternate (By
 MOD_RES 79 79 Phosphothreonine (By similarity).
 MOD_RES 86 86 N6-acetyllysine (By similarity).
 MOD_RES 99 99 N6-acetyllysine (By similarity).  

Keyword

 Acetylation; Chaperone; Complete proteome; Direct protein sequencing; Mitochondrion; Phosphoprotein; Reference proteome; Stress response. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 102 AA 

Protein Sequence

Gene Ontology

 GO:0005759; C:mitochondrial matrix; IEA:UniProtKB-SubCell.
 GO:0005739; C:mitochondrion; ISS:UniProtKB.
 GO:0005524; F:ATP binding; IEA:InterPro.
 GO:0051087; F:chaperone binding; ISS:UniProtKB.
 GO:0006457; P:protein folding; IEA:InterPro.
 GO:0006950; P:response to stress; IEA:UniProtKB-KW. 

Interpro

 IPR020818; Chaperonin_Cpn10.
 IPR018369; Chaprnonin_Cpn10_CS.
 IPR011032; GroES-like. 

Pfam

 PF00166; Cpn10 

SMART

 SM00883; Cpn10 

PROSITE

 PS00681; CHAPERONINS_CPN10 

PRINTS

 PR00297; CHAPERONIN10.