CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-044305
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 Integrator complex subunit 2 
Protein Synonyms/Alias
  
Gene Name
 INTS2 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
61QSWAQDKKLILRLLSubiquitination[1]
100KEQQLRHKLGGGSGEubiquitination[1, 2]
223EVCRGLIKNGERQDEubiquitination[1]
247DALRFLCKMNPSQALubiquitination[1]
255MNPSQALKVRGMVVEubiquitination[1, 2]
566TKHKVSIKDWIYRQLubiquitination[1, 2]
667TLAAMQRKPKSYSSSubiquitination[1]
669AAMQRKPKSYSSSLMubiquitination[1]
682LMDQIPIKFLIRQAQubiquitination[1]
742MLLTNNAKNHSPKQLubiquitination[1]
839IKFVRQQKYTQNDLMubiquitination[1, 2]
889AYLSAHLKETEQDRPubiquitination[1]
969ITTSAPNKGMEEGEDubiquitination[1, 3]
1087LTVLTQAKRYAFFMPubiquitination[4]
1146ITRLQQIKEKPSGWSubiquitination[1, 5]
1148RLQQIKEKPSGWSQIubiquitination[1, 6]
1157SGWSQICKDSSYKNGubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [4] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
 Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
 J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931]
 [5] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [6] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1196 AA 
Protein Sequence
MTECTSLQFV SPFAFEAMQK VDVVCLASLS DPELRLLLPC LVRMALCAPA DQSQSWAQDK 60
KLILRLLSGV EAVNSIVALL SVDFHALEQD ASKEQQLRHK LGGGSGESIL VSQLQHGLTL 120
EFEHSDSPRR LRLVLSELLA IMNKVSESNG EFFFKSSELF ESPVYLEEAA DVLCILQAEL 180
PSLLPIVDVA EALLHVRNGA WFLCLLVANV PDSFNEVCRG LIKNGERQDE ESLGGRRRTD 240
ALRFLCKMNP SQALKVRGMV VEECHLPGLG VALTLDHTKN EACEDGVSDL VCFVSGLLLG 300
TNAKVRTWFG TFIRNGQQRK RETSSSVLWQ MRRQLLLELM GILPTVRSTR IVEEADVDME 360
PNVSVYSGLK EEHVVKASAL LRLYCALMGI AGLKPTEEEA EQLLQLMTSR PPATPAGVRF 420
VSLSFCMLLA FSTLVSTPEQ EQLMVVWLSW MIKEEAYFES TSGVSASFGE MLLLVAMYFH 480
SNQLSAIIDL VCSTLGMKIV IKPSSLSRMK TIFTQEIFTE QVVTAHAVRV PVTSNLSANI 540
TGFLPIHCIY QLLRSRSFTK HKVSIKDWIY RQLCETSTPL HPQLLPLIDV YINSILTPAS 600
KSNPEATNQP VTEQEILNIF QGVIGGDNIR LNQRFSITAQ LLVLYYILSY EEALLANTKT 660
LAAMQRKPKS YSSSLMDQIP IKFLIRQAQG LQQELGGLHS ALLRLLATNY PHLCIVDDWI 720
CEEEITGTDA LLRRMLLTNN AKNHSPKQLQ EAFSAVPVNN TQVMQIIEHL TLLSASELIP 780
YAEVLTSNMS QLLNSGVPRR ILQTVNKLWM VLNTVMPRRL WVMTVNALQP SIKFVRQQKY 840
TQNDLMIDPL IVLRCDQRVH RCPPLMDITL HMLNGYLLAS KAYLSAHLKE TEQDRPSQNN 900
TIGLVGQTDA PEVTREELKN ALLAAQDSAA VQILLEICLP TEEEKANGVN PDSLLRNVQS 960
VITTSAPNKG MEEGEDNLLC NLREVQCLIC CLLHQMYIAD PNIAKLVHFQ GYPCELLPLT 1020
VAGIPSMHIC LDFIPELIAQ PELEKQIFAI QLLSHLCIQY ALPKSLSVAR LAVNVMGTLL 1080
TVLTQAKRYA FFMPTLPSLV SFCRAFPPLY EDIMSLLIQI GQVCASDVAT QTRDIDPIIT 1140
RLQQIKEKPS GWSQICKDSS YKNGSRDTGS MDPDVQLCHC IERTVIEIIN MSVSGI 1196 
Gene Ontology
 GO:0032039; C:integrator complex; IEA:InterPro.
 GO:0016180; P:snRNA processing; IEA:InterPro. 
Interpro
 IPR026236; Int2. 
Pfam
  
SMART
  
PROSITE
  
PRINTS
 PR02105; INTSUBUNIT2.