CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-019257
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Ceramide synthase 2 
Protein Synonyms/Alias
 CerS2; LAG1 longevity assurance homolog 2; SP260; Tumor metastasis-suppressor gene 1 protein 
Gene Name
 CERS2 
Gene Synonyms/Alias
 LASS2; TMSG1 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
93HFYLTSGKQPKQVEVubiquitination[1, 2, 3, 4]
96LTSGKQPKQVEVELLubiquitination[3]
131QDRPSLLKKFREASWubiquitination[2, 3]
334AHKFITGKLVEDERSubiquitination[2]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [4] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 
Functional Description
 Suppresses the growth of cancer cells. May be involved in sphingolipid synthesis. 
Sequence Annotation
 DOMAIN 131 332 TLC.
 DNA_BIND 67 128 Homeobox.
 MOD_RES 341 341 Phosphoserine.
 MOD_RES 346 346 Phosphothreonine.
 MOD_RES 348 348 Phosphoserine.
 MOD_RES 349 349 Phosphoserine.
 CARBOHYD 19 19 N-linked (GlcNAc...).  
Keyword
 Complete proteome; DNA-binding; Endoplasmic reticulum; Glycoprotein; Homeobox; Lipid biosynthesis; Lipid metabolism; Membrane; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Transmembrane; Transmembrane helix. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 380 AA 
Protein Sequence
MLQTLYDYFW WERLWLPVNL TWADLEDRDG RVYAKASDLY ITLPLALLFL IVRYFFELYV 60
ATPLAALLNI KEKTRLRAPP NATLEHFYLT SGKQPKQVEV ELLSRQSGLS GRQVERWFRR 120
RRNQDRPSLL KKFREASWRF TFYLIAFIAG MAVIVDKPWF YDMKKVWEGY PIQSTIPSQY 180
WYYMIELSFY WSLLFSIASD VKRKDFKEQI IHHVATIILI SFSWFANYIR AGTLIMALHD 240
SSDYLLESAK MFNYAGWKNT CNNIFIVFAI VFIITRLVIL PFWILHCTLV YPLELYPAFF 300
GYYFFNSMMG VLQLLHIFWA YLILRMAHKF ITGKLVEDER SDREETESSE GEEAAAGGGA 360
KSRPLANGHP ILNNNHRKND 380 
Gene Ontology
 GO:0005789; C:endoplasmic reticulum membrane; TAS:Reactome.
 GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
 GO:0031965; C:nuclear membrane; IDA:HPA.
 GO:0043565; F:sequence-specific DNA binding; IEA:InterPro.
 GO:0003700; F:sequence-specific DNA binding transcription factor activity; IEA:InterPro.
 GO:0050291; F:sphingosine N-acyltransferase activity; IEA:Compara.
 GO:0046513; P:ceramide biosynthetic process; IEA:Compara.
 GO:0044281; P:small molecule metabolic process; TAS:Reactome.
 GO:0030148; P:sphingolipid biosynthetic process; TAS:Reactome. 
Interpro
 IPR001356; Homeodomain.
 IPR009057; Homeodomain-like.
 IPR016439; Longevity_assurance_LAG1_LAC1.
 IPR006634; TLC-dom. 
Pfam
 PF00046; Homeobox
 PF03798; TRAM_LAG1_CLN8 
SMART
 SM00389; HOX
 SM00724; TLC 
PROSITE
 PS00027; HOMEOBOX_1
 PS50071; HOMEOBOX_2
 PS50922; TLC 
PRINTS