CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-001796
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 RNA polymerase sigma factor RpoD 
Protein Synonyms/Alias
 Sigma-70 
Gene Name
 rpoD 
Gene Synonyms/Alias
 alt; b3067; JW3039 
Created Date
 July 27, 2013 
Organism
 Escherichia coli (strain K12) 
NCBI Taxa ID
 83333 
Lysine Modification
Position
Peptide
Type
References
121EGEIDIAKRIEDGINacetylation[1]
251TAQEEILKLSEVFKQacetylation[1]
257LKLSEVFKQFRLVPKacetylation[1, 2, 3]
264KQFRLVPKQFDYLVNacetylation[1]
329MNKPWSEKLHDVSEEacetylation[1]
343EVHRALQKLQQIEEEacetylation[1]
359GLTIEQVKDINRRMSacetylation[1]
392RLVISIAKKYTNRGLacetylation[1]
418GLMKAVDKFEYRRGYacetylation[1]
462HMIETINKLNRISRQacetylation[1]
493RMLMPEDKIRKVLKIacetylation[1]
Reference
 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618]
 [2] The diversity of lysine-acetylated proteins in Escherichia coli.
 Yu BJ, Kim JA, Moon JH, Ryu SE, Pan JG.
 J Microbiol Biotechnol. 2008 Sep;18(9):1529-36. [PMID: 18852508]
 [3] Comprehensive profiling of protein lysine acetylation in Escherichia coli.
 Zhang K, Zheng S, Yang JS, Chen Y, Cheng Z.
 J Proteome Res. 2013 Feb 1;12(2):844-51. [PMID: 23294111] 
Functional Description
 Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is the primary sigma factor during exponential growth. Preferentially transcribes genes associated with fast growth, such as ribosomal operons, other protein-synthesis related genes, rRNA- and tRNA-encoding genes and prfB. 
Sequence Annotation
 DNA_BIND 573 592 H-T-H motif.
 REGION 2 80 Sigma-70 factor domain-1.
 REGION 379 449 Sigma-70 factor domain-2.
 REGION 458 534 Sigma-70 factor domain-3.
 REGION 547 600 Sigma-70 factor domain-4.
 REGION 584 599 Interaction with anti-sigma factors.
 MOTIF 403 406 Interaction with polymerase core subunit  
Keyword
 3D-structure; Complete proteome; Cytoplasm; DNA-binding; Reference proteome; Sigma factor; Transcription; Transcription regulation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 613 AA 
Protein Sequence
MEQNPQSQLK LLVTRGKEQG YLTYAEVNDH LPEDIVDSDQ IEDIIQMIND MGIQVMEEAP 60
DADDLMLAEN TADEDAAEAA AQVLSSVESE IGRTTDPVRM YMREMGTVEL LTREGEIDIA 120
KRIEDGINQV QCSVAEYPEA ITYLLEQYDR VEAEEARLSD LITGFVDPNA EEDLAPTATH 180
VGSELSQEDL DDDEDEDEED GDDDSADDDN SIDPELAREK FAELRAQYVV TRDTIKAKGR 240
SHATAQEEIL KLSEVFKQFR LVPKQFDYLV NSMRVMMDRV RTQERLIMKL CVEQCKMPKK 300
NFITLFTGNE TSDTWFNAAI AMNKPWSEKL HDVSEEVHRA LQKLQQIEEE TGLTIEQVKD 360
INRRMSIGEA KARRAKKEMV EANLRLVISI AKKYTNRGLQ FLDLIQEGNI GLMKAVDKFE 420
YRRGYKFSTY ATWWIRQAIT RSIADQARTI RIPVHMIETI NKLNRISRQM LQEMGREPTP 480
EELAERMLMP EDKIRKVLKI AKEPISMETP IGDDEDSHLG DFIEDTTLEL PLDSATTESL 540
RAATHDVLAG LTAREAKVLR MRFGIDMNTD YTLEEVGKQF DVTRERIRQI EAKALRKLRH 600
PSRSEVLRSF LDD 613 
Gene Ontology
 GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
 GO:0003700; F:sequence-specific DNA binding transcription factor activity; IEA:InterPro.
 GO:0016987; F:sigma factor activity; IDA:EcoliWiki.
 GO:0006352; P:DNA-dependent transcription, initiation; IEA:InterPro.
 GO:0009408; P:response to heat; IEP:EcoliWiki. 
Interpro
 IPR014284; RNA_pol_sigma-70_dom.
 IPR000943; RNA_pol_sigma70.
 IPR009042; RNA_pol_sigma70_r1_2.
 IPR007627; RNA_pol_sigma70_r2.
 IPR007624; RNA_pol_sigma70_r3.
 IPR007630; RNA_pol_sigma70_r4.
 IPR007631; RNA_pol_sigma_70_non-ess.
 IPR007127; RNA_pol_sigma_70_r1_1.
 IPR013325; RNA_pol_sigma_r2.
 IPR013324; RNA_pol_sigma_r3_r4.
 IPR012760; RNA_pol_sigma_RpoD_C.
 IPR011991; WHTH_DNA-bd_dom. 
Pfam
 PF04546; Sigma70_ner
 PF03979; Sigma70_r1_1
 PF00140; Sigma70_r1_2
 PF04542; Sigma70_r2
 PF04539; Sigma70_r3
 PF04545; Sigma70_r4 
SMART
  
PROSITE
 PS00715; SIGMA70_1
 PS00716; SIGMA70_2 
PRINTS
 PR00046; SIGMA70FCT.