UniProt Accession

 RGN_MOUSE; Q64374; A2AFC8; Q3UJG3; Q60944 

Genbank Protein ID

 U28937; U32170; D86217; AK146465; AL672073; BC012710 

Genbank Nucleotide ID

 AAC52721.1; AAD03478.1; BAA13046.1; BAE27192.1; CAM21274.1; AAH12710.1 

Protein Name

 Regucalcin 

Protein Synonyms/Alias

 RC; Gluconolactonase; GNL; Senescence marker protein 30; SMP-30 

Gene Name

 Rgn 

Gene Synonyms/Alias

 Smp30 

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
5	***MSSIKVECVLRE	acetylation	[1]
106	NNRFNDGKVDPAGRY	ubiquitination	[2]
144	LFPDHSVKKYFDQVD	acetylation	[3]
144	LFPDHSVKKYFDQVD	succinylation	[3]
144	LFPDHSVKKYFDQVD	ubiquitination	[2]
195	SNRRIVYKMEKDEQI	acetylation	[1, 4]
195	SNRRIVYKMEKDEQI	ubiquitination	[2]
198	RIVYKMEKDEQIPDG	acetylation	[4]
198	RIVYKMEKDEQIPDG	ubiquitination	[2]
213	MCIDAEGKLWVACYN	ubiquitination	[2]
233	RLDPETGKRLQTVKL	acetylation	[1]
233	RLDPETGKRLQTVKL	ubiquitination	[2]
239	GKRLQTVKLPVDKTT	acetylation	[1]
239	GKRLQTVKLPVDKTT	ubiquitination	[2]
244	TVKLPVDKTTSCCFG	acetylation	[3]
244	TVKLPVDKTTSCCFG	succinylation	[3]
244	TVKLPVDKTTSCCFG	ubiquitination	[2]
253	TSCCFGGKDYSEMYV	acetylation	[1, 3]
253	TSCCFGGKDYSEMYV	succinylation	[3]
253	TSCCFGGKDYSEMYV	ubiquitination	[2]
283	PDAGNIFKITGLGVK	ubiquitination	[2]

Reference

 [1] Calorie restriction and SIRT3 trigger global reprogramming of the mitochondrial protein acetylome.
 Hebert AS, Dittenhafer-Reed KE, Yu W, Bailey DJ, Selen ES, Boersma MD, Carson JJ, Tonelli M, Balloon AJ, Higbee AJ, Westphall MS, Pagliarini DJ, Prolla TA, Assadi-Porter F, Roy S, Denu JM, Coon JJ.
 Mol Cell. 2013 Jan 10;49(1):186-99. [PMID: 23201123]
 [2] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [3] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337]
 [4] Quantification of mitochondrial acetylation dynamics highlights prominent sites of metabolic regulation.
 Still AJ, Floyd BJ, Hebert AS, Bingman CA, Carson JJ, Gunderson DR, Dolan BK, Grimsrud PA, Dittenhafer-Reed KE, Stapleton DS, Keller MP, Westphall MS, Denu JM, Attie AD, Coon JJ, Pagliarini DJ.
 J Biol Chem. 2013 Jul 17;. [PMID: 23864654] 

Functional Description

 Gluconolactonase with low activity towards other sugar lactones, including gulonolactone and galactonolactone. Catalyzes a key step in ascorbic acid (vitamin C) biosynthesis. Can also hydrolyze diisopropyl phosphorofluoridate and phenylacetate (in vitro). Calcium-binding protein. Modulates Ca(2+) signaling, and Ca(2+)-dependent cellular processes and enzyme activities (By similarity). 

Sequence Annotation

 ACT_SITE 204 204 Proton donor/acceptor (Probable).
 METAL 18 18 Divalent metal cation.
 METAL 154 154 Divalent metal cation.
 METAL 204 204 Divalent metal cation.
 BINDING 101 101 Substrate.
 BINDING 103 103 Substrate.
 BINDING 121 121 Substrate.
 MOD_RES 3 3 Phosphoserine.  

Keyword

 3D-structure; Ascorbate biosynthesis; Calcium; Complete proteome; Cytoplasm; Hydrolase; Metal-binding; Phosphoprotein; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 299 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IDA:MGI.
 GO:0005634; C:nucleus; IDA:MGI.
 GO:0005509; F:calcium ion binding; ISS:UniProtKB.
 GO:0030234; F:enzyme regulator activity; IEA:InterPro.
 GO:0004341; F:gluconolactonase activity; IDA:UniProtKB.
 GO:0008270; F:zinc ion binding; ISS:UniProtKB.
 GO:0006874; P:cellular calcium ion homeostasis; ISS:UniProtKB.
 GO:0019853; P:L-ascorbic acid biosynthetic process; IDA:UniProtKB.
 GO:0032781; P:positive regulation of ATPase activity; ISS:UniProtKB.
 GO:0050848; P:regulation of calcium-mediated signaling; ISS:UniProtKB. 

Interpro

 IPR011042; 6-blade_b-propeller_TolB-like.
 IPR008367; Regucalcin.
 IPR013658; SGL.
 IPR005511; SMP-30. 

Pfam

 PF08450; SGL 

SMART

  

PROSITE

  

PRINTS

 PR01791; REGUCALCIN.
 PR01790; SMP30FAMILY.