CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-011600
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Calcium-activated potassium channel subunit alpha-1 
Protein Synonyms/Alias
 BK channel; BKCA alpha; Calcium-activated potassium channel, subfamily M subunit alpha-1; K(VCA)alpha; KCa1.1; Maxi K channel; MaxiK; Slo-alpha; Slo1; mSlo1; Slowpoke homolog; Slo homolog; mSlo 
Gene Name
 Kcnma1 
Gene Synonyms/Alias
 Kcnma 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
583FSMRSFIKIEEDTWQubiquitination[1]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 
Functional Description
 Potassium channel activated by both membrane depolarization or increase in cytosolic Ca(2+) that mediates export of K(+). It is also activated by the concentration of cytosolic Mg(2+). Its activation dampens the excitatory events that elevate the cytosolic Ca(2+) concentration and/or depolarize the cell membrane. It therefore contributes to repolarization of the membrane potential. Plays a key role in controlling excitability in a number of systems, such as regulation of the contraction of smooth muscle, the tuning of hair cells in the cochlea, regulation of transmitter release, and innate immunity. In smooth muscles, its activation by high level of Ca(2+), caused by ryanodine receptors in the sarcoplasmic reticulum, regulates the membrane potential. In cochlea cells, its number and kinetic properties partly determine the characteristic frequency of each hair cell and thereby helps to establish a tonotopic map. Kinetics of KCNMA1 channels are determined by alternative splicing, phosphorylation status and its combination with modulating beta subunits. Highly sensitive to both iberiotoxin (IbTx) and charybdotoxin (CTX). 
Sequence Annotation
 DOMAIN 415 558 RCK N-terminal.
 REGION 556 576 Segment S7.
 REGION 613 633 Segment S8.
 REGION 681 685 Heme-binding motif.
 REGION 783 803 Segment S9.
 REGION 1005 1025 Segment S10.
 MOTIF 352 355 Selectivity for potassium.
 MOTIF 976 998 Calcium bowl.
 METAL 439 439 Magnesium (Probable).
 METAL 462 462 Magnesium (Probable).
 METAL 464 464 Magnesium (Probable).
 METAL 985 985 Calcium; via carbonyl oxygen (By
 METAL 988 988 Calcium; via carbonyl oxygen (By
 METAL 991 991 Calcium (By similarity).
 METAL 993 993 Calcium (By similarity).
 MOD_RES 711 711 Phosphoserine.
 MOD_RES 1061 1061 Phosphothreonine.  
Keyword
 Alternative splicing; Calcium; Complete proteome; Ion channel; Ion transport; Magnesium; Membrane; Metal-binding; Phosphoprotein; Potassium; Potassium channel; Potassium transport; Reference proteome; Transmembrane; Transmembrane helix; Transport; Voltage-gated channel. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1209 AA 
Protein Sequence
MANGGGGGGG SSGGGGGGGG GSGLRMSSNI HANNLSLDAS SSSSSSSSSS SSSSSSSSSS 60
VHEPKMDALI IPVTMEVPCD SRGQRMWWAF LASSMVTFFG GLFIILLWRT LKYLWTVCCH 120
CGGKTKEAQK INNGSSQADG TLKPVDEKEE VVAAEVGWMT SVKDWAGVMI SAQTLTGRVL 180
VVLVFALSIG ALVIYFIDSS NPIESCQNFY KDFTLQIDMA FNVFFLLYFG LRFIAANDKL 240
WFWLEVNSVV DFFTVPPVFV SVYLNRSWLG LRFLRALRLI QFSEILQFLN ILKTSNSIKL 300
VNLLSIFIST WLTAAGFIHL VENSGDPWEN FQNNQALTYW ECVYLLMVTM STVGYGDVYA 360
KTTLGRLFMV FFILGGLAMF ASYVPEIIEL IGNRKKYGGS YSAVSGRKHI VVCGHITLES 420
VSNFLKDFLH KDRDDVNVEI VFLHNISPNL ELEALFKRHF TQVEFYQGSV LNPHDLARVK 480
IESADACLIL ANKYCADPDA EDASNIMRVI SIKNYHPKIR IITQMLQYHN KAHLLNIPSW 540
NWKEGDDAIC LAELKLGFIA QSCLAQGLST MLANLFSMRS FIKIEEDTWQ KYYLEGVSNE 600
MYTEYLSSAF VGLSFPTVCE LCFVKLKLLM IAIEYKSANR ESRILINPGN HLKIQEGTLG 660
FFIASDAKEV KRAFFYCKAC HDDVTDPKRI KKCGCRRPKM SIYKRMRRAC CFDCGRSERD 720
CSCMSGRVRG NVDTLERTFP LSSVSVNDCS TSFRAFEDEQ PPTLSPKKKQ RNGGMRNSPN 780
TSPKLMRHDP LLIPGNDQID NMDSNVKKYD STGMFHWCAP KEIEKVILTR SEAAMTVLSG 840
HVVVCIFGDV SSALIGLRNL VMPLRASNFH YHELKHIVFV GSIEYLKREW ETLHNFPKVS 900
ILPGTPLSRA DLRAVNINLC DMCVILSANQ NNIDDTSLQD KECILASLNI KSMQFDDSIG 960
VLQANSQGFT PPGMDRSSPD NSPVHGMLRQ PSITTGVNIP IITELVNDTN VQFLDQDDDD 1020
DPDTELYLTQ PFACGTAFAV SVLDSLMSAT YFNDNILTLI RTLVTGGATP ELEALIAEEN 1080
ALRGGYSTPQ TLANRDRCRV AQLALLDGPF ADLGDGGCYG DLFCKALKTY NMLCFGIYRL 1140
RDAHLSTPSQ CTKRYVITNP PYEFELVPTD LIFCLMQFDH NAGQSRASLS HSSHSSQSSS 1200
KKSSSVHSIP STANRPNRPK SRESRDKQNR KEMVYR 1236 
Gene Ontology
 GO:0016324; C:apical plasma membrane; IDA:MGI.
 GO:0005783; C:endoplasmic reticulum; IDA:MGI.
 GO:0009897; C:external side of plasma membrane; IDA:MGI.
 GO:0045211; C:postsynaptic membrane; IDA:MGI.
 GO:0043195; C:terminal bouton; IDA:MGI.
 GO:0008076; C:voltage-gated potassium channel complex; IEA:InterPro.
 GO:0060072; F:large conductance calcium-activated potassium channel activity; IDA:MGI.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0005249; F:voltage-gated potassium channel activity; IDA:MGI.
 GO:0007628; P:adult walking behavior; IMP:MGI.
 GO:0042491; P:auditory receptor cell differentiation; IMP:MGI.
 GO:0048469; P:cell maturation; IMP:MGI.
 GO:0060082; P:eye blink reflex; IMP:MGI.
 GO:0045475; P:locomotor rhythm; IMP:MGI.
 GO:0045794; P:negative regulation of cell volume; IMP:MGI.
 GO:0050885; P:neuromuscular process controlling balance; IMP:MGI.
 GO:0051260; P:protein homooligomerization; IDA:MGI.
 GO:0019228; P:regulation of action potential in neuron; IMP:MGI.
 GO:0032344; P:regulation of aldosterone metabolic process; IMP:MGI.
 GO:0060087; P:relaxation of vascular smooth muscle; IMP:MGI.
 GO:0001666; P:response to hypoxia; IDA:MGI.
 GO:0046541; P:saliva secretion; IGI:MGI.
 GO:0007605; P:sensory perception of sound; IMP:MGI.
 GO:0060083; P:smooth muscle contraction involved in micturition; IMP:MGI.
 GO:0007268; P:synaptic transmission; IMP:MGI. 
Interpro
 IPR024939; Ca-act_K_channel_Slo.
 IPR005821; Ion_trans_dom.
 IPR003091; K_chnl.
 IPR003929; K_chnl_Ca-activ_BK_asu.
 IPR016040; NAD(P)-bd_dom.
 IPR003148; RCK_N. 
Pfam
 PF03493; BK_channel_a
 PF00520; Ion_trans
 PF02254; TrkA_N 
SMART
  
PROSITE
 PS51201; RCK_N 
PRINTS
 PR01449; BKCHANNELA.
 PR00169; KCHANNEL.