CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-022239
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Kelch repeat and BTB domain-containing protein 4 
Protein Synonyms/Alias
 BTB and kelch domain-containing protein 4 
Gene Name
 KBTBD4 
Gene Synonyms/Alias
 BKLHD4 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
232ESLRTSLKEIGENVHubiquitination[1, 2, 3, 4, 5]
245VHIYLIGKESSRTHSubiquitination[4]
300SIPRRMWKCNNATVDubiquitination[4]
329TLVSVPGKDAIYSLGubiquitination[3, 4, 5]
338AIYSLGGKTLQDTLSubiquitination[2, 3]
409CFDTETDKCHVKPYVubiquitination[4]
413ETDKCHVKPYVLPFAubiquitination[4]
492KGDANTYKLDPATSAubiquitination[2, 5]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [4] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [5] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302
Functional Description
  
Sequence Annotation
 DOMAIN 45 112 BTB.
 DOMAIN 147 239 BACK.
 REPEAT 239 285 Kelch 1.
 REPEAT 286 328 Kelch 2.
 REPEAT 331 378 Kelch 3.
 REPEAT 380 430 Kelch 4.
 REPEAT 432 481 Kelch 5.  
Keyword
 3D-structure; Alternative splicing; Complete proteome; Kelch repeat; Polymorphism; Reference proteome; Repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 518 AA 
Protein Sequence
MESPEEPGAS MDENYFVNYT FKDRSHSGRV AQGIMKLCLE EELFADVTIS VEGREFQLHR 60
LVLSAQSCFF RSMFTSNLKE AHNRVIVLQD VSESVFQLLV DYIYHGTVKL RAEELQEIYE 120
VSDMYQLTSL FEECSRFLAR TVQVGNCLQV MWLADRHSDP ELYTAAKHCA KTHLAQLQNT 180
EEFLHLPHRL LTDIISDGVP CSQNPTEAIE AWINFNKEER EAFAESLRTS LKEIGENVHI 240
YLIGKESSRT HSLAVSLHCA EDDSISVSGQ NSLCHQITAA CKHGGDLYVV GGSIPRRMWK 300
CNNATVDWEW CAPLPRDRLQ HTLVSVPGKD AIYSLGGKTL QDTLSNAVIY YRVGDNVWTE 360
TTQLEVAVSG AAGANLNGII YLLGGEENDL DFFTKPSRLI QCFDTETDKC HVKPYVLPFA 420
GRMHAAVHKD LVFIVAEGDS LVCYNPLLDS FTRLCLPEAW SSAPSLWKIA SCNGSIYVFR 480
DRYKKGDANT YKLDPATSAV TVTRGIKVLL TNLQFVLA 518 
Gene Ontology
  
Interpro
 IPR011705; BACK.
 IPR000210; BTB/POZ-like.
 IPR011333; BTB/POZ_fold.
 IPR013069; BTB_POZ.
 IPR015915; Kelch-typ_b-propeller. 
Pfam
 PF07707; BACK
 PF00651; BTB 
SMART
 SM00875; BACK
 SM00225; BTB 
PROSITE
 PS50097; BTB 
PRINTS