| Tag | Content |
|---|
| CPLM ID | CPLM-002310 |
UniProt Accession | |
Genbank Protein ID | |
Genbank Nucleotide ID | |
Protein Name | General negative regulator of transcription subunit 3 |
Protein Synonyms/Alias | |
Gene Name | NOT3 |
Gene Synonyms/Alias | YIL038C |
Created Date | July 27, 2013 |
Organism | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
NCBI Taxa ID | 559292 |
Lysine Modification | Position | Peptide | Type | References |
|---|
| 110 | YSNISLKKSETLDPQ | ubiquitination | [1] | | 290 | KAAKLAAKNATGAAI | ubiquitination | [1] | | 317 | IPVADASKETERSPS | ubiquitination | [1] | | 343 | EAVKTSIKSPRSSAD | ubiquitination | [1] | | 413 | AASQAVEKDRKVTSA | ubiquitination | [1] | | 416 | QAVEKDRKVTSASST | ubiquitination | [1] | | 430 | TISNTSTKTPTTAAA | ubiquitination | [1, 2] | | 534 | NGPENTTKKVMEQKE | ubiquitination | [1] | | 535 | GPENTTKKVMEQKEE | ubiquitination | [2] | | 540 | TKKVMEQKEEESPEE | ubiquitination | [1] | | 686 | IIGSERLKEDSSSIY | ubiquitination | [1] | | 816 | NVTLEQQKQEISHGK | ubiquitination | [1] |
|
Reference | [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation. Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J. Nat Methods. 2013 Jul;10(7):676-82. [ PMID: 23749301] [2] Sites of ubiquitin attachment in Saccharomyces cerevisiae. Starita LM, Lo RS, Eng JK, von Haller PD, Fields S. Proteomics. 2012 Jan;12(2):236-40. [ PMID: 22106047] |
Functional Description | Acts as component of the CCR4-NOT core complex, which in the nucleus seems to be a general transcription factor, and in the cytoplasm the major mRNA deadenylase involved in mRNA turnover. The NOT protein subcomplex negatively regulates the basal and activated transcription of many genes. Preferentially affects TC- type TATA element-dependent transcription. Could directly or indirectly inhibit component(s) of the general transcription machinery. |
Sequence Annotation | MOD_RES 303 303 Phosphoserine.
MOD_RES 307 307 Phosphoserine.
MOD_RES 446 446 Phosphoserine.
MOD_RES 450 450 Phosphoserine.
MOD_RES 454 454 Phosphothreonine.
MOD_RES 565 565 Phosphoserine.
MOD_RES 569 569 Phosphoserine.
MOD_RES 571 571 Phosphothreonine.
MOD_RES 657 657 Phosphoserine. |
Keyword | Activator; Coiled coil; Complete proteome; Cytoplasm; Nucleus; Phosphoprotein; Reference proteome; Repressor; Transcription; Transcription regulation. |
Sequence Source | UniProt (SWISSPROT/TrEMBL); GenBank; EMBL |
Protein Length | 836 AA |
Protein Sequence | MAHRKLQQEV DRVFKKINEG LEIFNSYYER HESCTNNPSQ KDKLESDLKR EVKKLQRLRE 60
QIKSWQSSPD IKDKDSLLDY RRSVEIAMEK YKAVEKASKE KAYSNISLKK SETLDPQERE 120
RRDISEYLSQ MIDELERQYD SLQVEIDKLL LLNKKKKTSS TTNDEKKEQY KRFQARYRWH 180
QQQMELALRL LANEELDPQD VKNVQDDINY FVESNQDPDF VEDETIYDGL NLQSNEAIAH 240
EVAQYFASQN AEDNNTSDAN ESLQDISKLS KKEQRKLERE AKKAAKLAAK NATGAAIPVA 300
GPSSTPSPVI PVADASKETE RSPSSSPIHN ATKPEEAVKT SIKSPRSSAD NLLPSLQKSP 360
SSATPETPTN VHTHIHQTPN GITGATTLKP ATLPAKPAGE LKWAVAASQA VEKDRKVTSA 420
SSTISNTSTK TPTTAAATTT SSNANSRIGS ALNTPKLSTS SLSLQPDNTG ASSSAATAAA 480
VLAAGAAAVH QNNQAFYRNM SSSHHPLVSL ATNPKSEHEV ATTVNQNGPE NTTKKVMEQK 540
EEESPEERNK LQVPTFGVFD DDFESDRDSE TEPEEEEQPS TPKYLSLEQR EAKTNEIKKE 600
FVSDFETLLL PSGVQEFIMS SELYNSQIES KITYKRSRDM CEISRLVEVP QGVNPPSPLD 660
AFRSTQQWDV MRCSLRDIII GSERLKEDSS SIYAKILENF RTLEMFSLFY NYYFAITPLE 720
REIAYKILNE RDWKVSKDGT MWFLRQGEVK FFNEICEVGD YKIFKLDDWT VIDKINFRLD 780
YSFLQPPVDT ASEVRDVSVD NNNVNDQSNV TLEQQKQEIS HGKQLLKQLK QGKISV 836 |
Gene Ontology | GO:0030015; C:CCR4-NOT core complex; IPI:SGD. GO:0005737; C:cytoplasm; IDA:SGD. GO:0005634; C:nucleus; IEA:UniProtKB-SubCell. GO:0000289; P:nuclear-transcribed mRNA poly(A) tail shortening; IDA:SGD. GO:0032968; P:positive regulation of transcription elongation from RNA polymerase II promoter; IDA:SGD. GO:0016567; P:protein ubiquitination; IMP:SGD. GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. |
Interpro | |
Pfam | |
SMART | |
PROSITE | |
PRINTS | |