UniProt Accession

 SGOL1_HUMAN; Q5FBB7; Q588H5; Q5FBB4; Q5FBB5; Q5FBB6; Q5FBB8; Q8N579; Q8WVL0; Q9BVA8; Q9H275 

Genbank Protein ID

 AB190994; AB193056; AB193057; AB193058; AB193059; AB193060; AB193061; AB193062; AB193063; AB193064; AB193065; AB193066; AB187577; AB187578; AB187579; AB187580; AB187581; AB187582; BC001339; BC017867; BC032696; AF308299 

Genbank Nucleotide ID

 BAD91318.1; BAD95529.1; BAD95530.1; BAD95531.1; BAD95532.1; BAD95533.1; BAD95534.1; BAD95535.1; BAD95536.1; BAD95537.1; BAD95538.1; BAD95539.1; BAD89587.1; BAD89588.1; BAD89589.1; BAD89590.1; BAD89591.1; BAD89592.1; AAH01339.2; AAH17867.1; AAH32696.1; AAG48266.1 

Protein Name

 Shugoshin-like 1 

Protein Synonyms/Alias

 hSgo1; Serologically defined breast cancer antigen NY-BR-85 

Gene Name

 SGOL1 

Gene Synonyms/Alias

 SGO1 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
454	VSLYPVVKIRRLSLS	ubiquitination	[1, 2, 3]
463	RRLSLSPKKNKASPA	ubiquitination	[4]
464	RLSLSPKKNKASPAV	ubiquitination	[4]
466	SLSPKKNKASPAVAL	ubiquitination	[4]

Reference

 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [3] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [4] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 

Functional Description

 Plays a central role in chromosome cohesion during mitosis by preventing premature dissociation of cohesin complex from centromeres after prophase, when most of cohesin complex dissociates from chromosomes arms. May act by preventing phosphorylation of the STAG2 subunit of cohesin complex at the centromere, ensuring cohesin persistence at centromere until cohesin cleavage by ESPL1/separase at anaphase. Essential for proper chromosome segregation during mitosis and this function requires interaction with PPP2R1A. Its phosphorylated form is necessary for chromosome congression and for the proper attachment of spindle microtubule to the kinetochore. Necessary for kinetochore localization of PLK1 and CENPF. May play a role in the tension sensing mechanism of the spindle-assembly checkpoint by regulating PLK1 kinetochore affinity. Isoform 3 plays a role in maintaining centriole cohesion involved in controlling spindle pole integrity. 

Sequence Annotation

 REGION 1 176 Necessary for interaction with PPP2CA and
 MOTIF 192 200 D-box 1.
 MOTIF 310 312 KEN box.
 MOTIF 438 446 D-box 2.
 MOTIF 457 465 D-box 3.
 MOD_RES 14 14 Phosphoserine; by NEK2.
 MOD_RES 256 256 Phosphoserine.
 MOD_RES 436 436 Phosphoserine.
 MOD_RES 507 507 Phosphoserine; by NEK2.  

Keyword

 3D-structure; Alternative splicing; Cell cycle; Cell division; Centromere; Chromosome; Chromosome partition; Coiled coil; Complete proteome; Cytoplasm; Cytoskeleton; Kinetochore; Mitosis; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Ubl conjugation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 561 AA 

Protein Sequence

Gene Ontology

 GO:0005813; C:centrosome; IDA:UniProtKB.
 GO:0000777; C:condensed chromosome kinetochore; IEA:UniProtKB-SubCell.
 GO:0000779; C:condensed chromosome, centromeric region; IDA:UniProtKB.
 GO:0000780; C:condensed nuclear chromosome, centromeric region; IEA:Compara.
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0000776; C:kinetochore; IDA:UniProtKB.
 GO:0030892; C:mitotic cohesin complex; IDA:MGI.
 GO:0000922; C:spindle pole; IDA:UniProtKB.
 GO:0008608; P:attachment of spindle microtubules to kinetochore; IDA:UniProtKB.
 GO:0051301; P:cell division; IEA:UniProtKB-KW.
 GO:0010457; P:centriole-centriole cohesion; IDA:UniProtKB.
 GO:0045132; P:meiotic chromosome segregation; IEA:InterPro.
 GO:0007067; P:mitosis; IEA:UniProtKB-KW.
 GO:0000278; P:mitotic cell cycle; TAS:Reactome. 

Interpro

 IPR011515; Shugoshin_C.
 IPR011516; Shugoshin_N. 

Pfam

 PF07557; Shugoshin_C
 PF07558; Shugoshin_N 

SMART

  

PROSITE

  

PRINTS