CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-032909
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 26S proteasome non-ATPase regulatory subunit 6 
Protein Synonyms/Alias
 Proteasome (Prosome, macropain) 26S subunit, non-ATPase, 6, isoform CRA_b 
Gene Name
 PSMD6 
Gene Synonyms/Alias
 hCG_23509 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
13LEEEGLPKNPDLRIAubiquitination[1]
91SIRLCFAKPIIKRHRubiquitination[1]
95CFAKPIIKRHRMPSYubiquitination[1]
146EELEDAEKNLGESEIubiquitination[2]
160IRDAMMAKAEYLCRIubiquitination[1, 2, 3, 4, 5]
170YLCRIGDKEGALTAFubiquitination[1, 5]
183AFRKTYDKTVALGHRubiquitination[1, 2]
218TRNTEKAKSLIEEGGubiquitination[1]
234WDRRNRLKVYQGLYCubiquitination[5]
295DLREKVIKGAEILEVubiquitination[1, 2, 3, 6]
337VVEQEMKKDWLFAPHubiquitination[1]
399AAGRLHCKIDKVNEIubiquitination[3]
402RLHCKIDKVNEIVETubiquitination[1, 2, 5]
415ETNRPDSKNWQYQETubiquitination[1, 2, 3, 6, 7, 8]
424WQYQETIKKGDLLLNubiquitination[1, 2, 5, 6]
425QYQETIKKGDLLLNRubiquitination[1, 2, 5]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [4] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [5] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [6] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [7] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [8] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Proteasome; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 442 AA 
Protein Sequence
MPLENLEEEG LPKNPDLRIA QLRFLLSLPE HRGDAAVRDE LMAAVRDNNF WNRIRQEQTE 60
DCPPSSPSRG WHCQDWPSRK PPTSIRLCFA KPIIKRHRMP SYMAPYYEAL CKSLDWQIDV 120
DLLNKMKKAN EDELKRLDEE LEDAEKNLGE SEIRDAMMAK AEYLCRIGDK EGALTAFRKT 180
YDKTVALGHR LDIVFYLLRI GLFYMDNDLI TRNTEKAKSL IEEGGDWDRR NRLKVYQGLY 240
CVAIRDFKQA AELFLDTVST FTSYELMDYK TFVTYTVYVS MIALERPDLR EKVIKGAEIL 300
EVLHSLPAVR QYLFSLYECR YSVFFQSLAV VEQEMKKDWL FAPHYRYYVR EMRIHAYSQL 360
LESYRSLTLG YMAEAFGVGV EFIDQELSRF IAAGRLHCKI DKVNEIVETN RPDSKNWQYQ 420
ETIKKGDLLL NRVQKLSRVI NM 442 
Gene Ontology
 GO:0000502; C:proteasome complex; IEA:UniProtKB-KW. 
Interpro
 IPR019585; 26S_proteasome_reg_su-Rpn7.
 IPR000717; PCI_dom.
 IPR011991; WHTH_DNA-bd_dom. 
Pfam
 PF01399; PCI
 PF10602; RPN7 
SMART
 SM00088; PINT 
PROSITE
  
PRINTS