UniProt Accession

 BUB3_HUMAN; O43684; A6NJ42; B2R6E7; D3DRE9; O43685 

Genbank Protein ID

 AF047472; AF047473; AF053304; AF081496; AK312546; AC012391; CH471066; CH471066; CH471066; BC005138; BC022438 

Genbank Nucleotide ID

 AAC28438.1; AAC28439.1; AAC06258.1; AAC36307.1; BAG35444.1; EAW49284.1; EAW49285.1; EAW49286.1; AAH05138.1; AAH22438.1 

Protein Name

 Mitotic checkpoint protein BUB3 

Protein Synonyms/Alias

  

Gene Name

 BUB3 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
179	QRRESSLKYQTRCIR	ubiquitination	[1]
191	CIRAFPNKQGYVLSS	ubiquitination	[1]
216	DPSPEVQKKKYAFKC	ubiquitination	[1]
227	AFKCHRLKENNIEQI	ubiquitination	[1]
322	QVTDAETKPKST***	ubiquitination	[2]
324	TDAETKPKST*****	ubiquitination	[2]

Reference

 [1] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 

Functional Description

 Has a dual function in spindle-assembly checkpoint signaling and in promoting the establishment of correct kinetochore-microtubule (K-MT) attachments. Promotes the formation of stable end-on bipolar attachments. Necessary for kinetochore localization of BUB1. Regulates chromosome segregation during oocyte meiosis. The BUB1/BUB3 complex plays a role in the inhibition of anaphase-promoting complex or cyclosome (APC/C) when spindle-assembly checkpoint is activated and inhibits the ubiquitin ligase activity of APC/C by phosphorylating its activator CDC20. This complex can also phosphorylate MAD1L1. 

Sequence Annotation

 REPEAT 5 43 WD 1.
 REPEAT 46 83 WD 2.
 REPEAT 86 124 WD 3.
 REPEAT 128 163 WD 4.
 REPEAT 223 262 WD 5.
 MOD_RES 179 179 N6-acetyllysine.
 MOD_RES 211 211 Phosphoserine.
 CROSSLNK 216 216 Glycyl lysine isopeptide (Lys-Gly)  

Keyword

 Acetylation; ADP-ribosylation; Alternative splicing; Cell cycle; Cell division; Centromere; Chromosome; Chromosome partition; Complete proteome; Isopeptide bond; Kinetochore; Meiosis; Mitosis; Nucleus; Phosphoprotein; Reference proteome; Repeat; Ubl conjugation; WD repeat. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 328 AA 

Protein Sequence

Gene Ontology

 GO:0000777; C:condensed chromosome kinetochore; IEA:UniProtKB-SubCell.
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0000776; C:kinetochore; IDA:UniProtKB.
 GO:0005634; C:nucleus; IDA:HPA.
 GO:0031145; P:anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; TAS:Reactome.
 GO:0008608; P:attachment of spindle microtubules to kinetochore; IDA:UniProtKB.
 GO:0051301; P:cell division; IEA:UniProtKB-KW.
 GO:0007126; P:meiosis; IEA:UniProtKB-KW.
 GO:0000070; P:mitotic sister chromatid segregation; IEA:Compara.
 GO:0007094; P:mitotic spindle assembly checkpoint; TAS:Reactome.
 GO:0051436; P:negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle; TAS:Reactome.
 GO:0051983; P:regulation of chromosome segregation; IEA:Compara. 

Interpro

 IPR020472; G-protein_beta_WD-40_rep.
 IPR015943; WD40/YVTN_repeat-like_dom.
 IPR001680; WD40_repeat.
 IPR017986; WD40_repeat_dom. 

Pfam

 PF00400; WD40 

SMART

 SM00320; WD40 

PROSITE

 PS00678; WD_REPEATS_1
 PS50082; WD_REPEATS_2
 PS50294; WD_REPEATS_REGION 

PRINTS

 PR00320; GPROTEINBRPT.