CPLM-008310

Genbank Nucleotide ID

Very long-chain specific acyl-CoA dehydrogenase, mitochondrial

Protein Synonyms/Alias

Mus musculus (Mouse)

Position	Peptide	Type	References
52	ATQAVLDKPETLSSD	acetylation	[1, 2, 3, 4, 5]
72	KPARAESKSFAVGMF	acetylation	[3, 4, 5, 6]
72	KPARAESKSFAVGMF	succinylation	[6]
122	EEVNDPAKNDALEKV	acetylation	[3]
122	EEVNDPAKNDALEKV	ubiquitination	[7]
128	AKNDALEKVEDDTLQ	acetylation	[3]
128	AKNDALEKVEDDTLQ	ubiquitination	[7]
201	GTKAQREKYLPRVAS	acetylation	[3]
240	AIPSPCGKYYTLNGS	acetylation	[3, 8]
240	AIPSPCGKYYTLNGS	ubiquitination	[7]
277	DAATGAVKEKITAFV	acetylation	[3, 8]
279	ATGAVKEKITAFVVE	acetylation	[3]
299	VTHGLPEKKMGIKAS	acetylation	[3]
299	VTHGLPEKKMGIKAS	ubiquitination	[7]
317	EVYFDGVKVPSENVL	ubiquitination	[7]
332	GEVGDGFKVAVNILN	ubiquitination	[9]
359	TMKSLIAKAVDHATN	ubiquitination	[7]
373	NRTQFGDKIHNFGVI	acetylation	[3]
373	NRTQFGDKIHNFGVI	ubiquitination	[7]
483	QGCMDKGKELTGLGN	acetylation	[3]
508	LLMGEAGKQLRRRTG	ubiquitination	[7]

[1] The fasted/fed mouse metabolic acetylome: N6-acetylation differences suggest acetylation coordinates organ-specific fuel switching.
Yang L, Vaitheesvaran B, Hartil K, Robinson AJ, Hoopmann MR, Eng JK, Kurland IJ, Bruce JE.
J Proteome Res. 2011 Sep 2;10(9):4134-49. [PMID: 21728379]
[2] Quantitative assessment of the impact of the gut microbiota on lysine epsilon-acetylation of host proteins using gnotobiotic mice.
Simon GM, Cheng J, Gordon JI.
Proc Natl Acad Sci U S A. 2012 Jul 10;109(28):11133-8. [PMID: 22733758]
[3] Calorie restriction and SIRT3 trigger global reprogramming of the mitochondrial protein acetylome.
Hebert AS, Dittenhafer-Reed KE, Yu W, Bailey DJ, Selen ES, Boersma MD, Carson JJ, Tonelli M, Balloon AJ, Higbee AJ, Westphall MS, Pagliarini DJ, Prolla TA, Assadi-Porter F, Roy S, Denu JM, Coon JJ.
Mol Cell. 2013 Jan 10;49(1):186-99. [PMID: 23201123]
[4] Label-free quantitative proteomics of the lysine acetylome in mitochondria identifies substrates of SIRT3 in metabolic pathways.
Rardin MJ, Newman JC, Held JM, Cusack MP, Sorensen DJ, Li B, Schilling B, Mooney SD, Kahn CR, Verdin E, Gibson BW.
Proc Natl Acad Sci U S A. 2013 Apr 16;110(16):6601-6. [PMID: 23576753]
[5] Quantification of mitochondrial acetylation dynamics highlights prominent sites of metabolic regulation.
Still AJ, Floyd BJ, Hebert AS, Bingman CA, Carson JJ, Gunderson DR, Dolan BK, Grimsrud PA, Dittenhafer-Reed KE, Stapleton DS, Keller MP, Westphall MS, Denu JM, Attie AD, Coon JJ, Pagliarini DJ.
J Biol Chem. 2013 Jul 17;. [PMID: 23864654]
[6] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337]
[7] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
[8] Substrate and functional diversity of lysine acetylation revealed by a proteomics survey.
Kim SC, Sprung R, Chen Y, Xu Y, Ball H, Pei J, Cheng T, Kho Y, Xiao H, Xiao L, Grishin NV, White M, Yang XJ, Zhao Y.
Mol Cell. 2006 Aug;23(4):607-18. [PMID: 16916647]
[9] A proteomics approach to identify the ubiquitinated proteins in mouse heart.
Jeon HB, Choi ES, Yoon JH, Hwang JH, Chang JW, Lee EK, Choi HW, Park ZY, Yoo YJ.
Biochem Biophys Res Commun. 2007 Jun 8;357(3):731-6. [PMID: 17451654]

Functional Description

Active toward esters of long-chain and very long chain fatty acids such as palmitoyl-CoA, mysritoyl-CoA and stearoyl-CoA. Can accommodate substrate acyl chain lengths as long as 24 carbons, but shows little activity for substrates of less than 12 carbons (By similarity).

NP_BIND 215 224 FAD (By similarity).
NP_BIND 250 252 FAD (By similarity).
NP_BIND 436 440 FAD (By similarity).
NP_BIND 465 467 FAD (By similarity).
REGION 42 483 Catalytic.
REGION 339 342 Substrate binding (By similarity).
REGION 463 464 Substrate binding (By similarity).
REGION 484 517 Membrane-anchoring (Probable).
ACT_SITE 463 463 Proton acceptor (By similarity).
BINDING 224 224 Substrate; via carbonyl oxygen (By
BINDING 367 367 FAD (By similarity).
BINDING 464 464 Substrate; via amide nitrogen (By
MOD_RES 238 238 S-nitrosocysteine.
MOD_RES 240 240 N6-acetyllysine.
MOD_RES 277 277 N6-acetyllysine.
MOD_RES 332 332 N6-acetyllysine; alternate (By
CROSSLNK 332 332 Glycyl lysine isopeptide (Lys-Gly)

Acetylation; Complete proteome; FAD; Fatty acid metabolism; Flavoprotein; Isopeptide bond; Lipid metabolism; Membrane; Mitochondrion; Mitochondrion inner membrane; Oxidoreductase; Reference proteome; S-nitrosylation; Transit peptide; Ubl conjugation.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0005743; C:mitochondrial inner membrane; IEA:UniProtKB-SubCell.
GO:0005739; C:mitochondrion; IDA:MGI.
GO:0003995; F:acyl-CoA dehydrogenase activity; IEA:InterPro.
GO:0050660; F:flavin adenine dinucleotide binding; IEA:InterPro.
GO:0033539; P:fatty acid beta-oxidation using acyl-CoA dehydrogenase; IMP:BHF-UCL.
GO:0045717; P:negative regulation of fatty acid biosynthetic process; IMP:BHF-UCL.
GO:0046322; P:negative regulation of fatty acid oxidation; IMP:BHF-UCL.
GO:0090181; P:regulation of cholesterol metabolic process; IMP:BHF-UCL.
GO:0001659; P:temperature homeostasis; IMP:BHF-UCL.

IPR006089; Acyl-CoA_DH_CS.
IPR006092; Acyl-CoA_DH_N.
IPR006090; Acyl-CoA_Oxase/DH_1.
IPR006091; Acyl-CoA_Oxase/DH_cen-dom.
IPR009075; AcylCo_DH/oxidase_C.
IPR013786; AcylCoA_DH/ox_N.
IPR009100; AcylCoA_DH/oxidase.

PF00441; Acyl-CoA_dh_1
PF02770; Acyl-CoA_dh_M
PF02771; Acyl-CoA_dh_N

PS00072; ACYL_COA_DH_1
PS00073; ACYL_COA_DH_2