CPLM-009393

Genbank Nucleotide ID

DDB1- and CUL4-associated factor 7

Protein Synonyms/Alias

WD repeat-containing protein 68; WD repeat-containing protein An11 homolog

Homo sapiens (Human)

Position	Peptide	Type	References
30	NWSVRPDKRFRLALG	ubiquitination	[1, 2, 3]
73	DHPYPTTKLMWIPDT	ubiquitination	[1, 2, 3, 4, 5, 6]
81	LMWIPDTKGVYPDLL	ubiquitination	[1, 2, 3, 4, 5, 6, 7]
167	NLVSGHVKTQLIAHD	ubiquitination	[1, 2, 3, 4, 8, 9]
175	TQLIAHDKEVYDIAF	ubiquitination	[2]

[1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
[2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
[3] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
Chen Z, Zhou Y, Song J, Zhang Z.
Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
[4] Systematic and quantitative assessment of the ubiquitin-modified proteome.
Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
[5] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
[6] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
[7] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572]
[8] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931]
[9] Integrated proteomic analysis of post-translational modifications by serial enrichment.
Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]

Functional Description

Involved in craniofacial development. Acts upstream of the EDN1 pathway and is required for formation of the upper jaw equivalent, the palatoquadrate. The activity required for EDN1 pathway function differs between the first and second arches (By similarity). Associates with DIAPH1 and controls GLI1 transcriptional activity. Could be involved in normal and disease skin development. May function as a substrate receptor for CUL4- DDB1 E3 ubiquitin-protein ligase complex.

REPEAT 66 108 WD 1.
REPEAT 118 160 WD 2.
REPEAT 172 214 WD 3.
REPEAT 264 304 WD 4.

Complete proteome; Cytoplasm; Developmental protein; Nucleus; Reference proteome; Repeat; Ubl conjugation pathway; WD repeat.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0080008; C:Cul4-RING ubiquitin ligase complex; IDA:UniProtKB.
GO:0005737; C:cytoplasm; TAS:UniProtKB.
GO:0005634; C:nucleus; IDA:HPA.
GO:0007275; P:multicellular organismal development; IEA:UniProtKB-KW.
GO:0016567; P:protein ubiquitination; IC:UniProtKB.

IPR015943; WD40/YVTN_repeat-like_dom.
IPR001680; WD40_repeat.
IPR019775; WD40_repeat_CS.
IPR017986; WD40_repeat_dom.

PS00678; WD_REPEATS_1
PS50082; WD_REPEATS_2
PS50294; WD_REPEATS_REGION