CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-015358
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 WD repeat-containing protein 74 
Protein Synonyms/Alias
 NOP seven-associated protein 1 
Gene Name
 WDR74 
Gene Synonyms/Alias
 NSA1 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
21GTETGILKGVNLQRKubiquitination[1]
28KGVNLQRKQAANFTAubiquitination[1, 2]
68GCADRTVKHFSTEDGubiquitination[1, 2, 3, 4, 5, 6]
115ILRVWHDKDKDTSSDubiquitination[1, 2]
117RVWHDKDKDTSSDPLubiquitination[2]
151HVVATGGKENALKIWubiquitination[1]
156GGKENALKIWDLQGSubiquitination[1, 2, 4, 5]
197QFLPGSQKLVTCTGYubiquitination[1, 5]
266GRLLGCLKGLAGSVRubiquitination[1, 2, 4, 5]
281GLQCHPSKPLLASCGubiquitination[1, 2]
307NPRGLEHKVYLKSQLubiquitination[1]
311LEHKVYLKSQLNCLLubiquitination[1, 2]
337QEPQEPNKVPLEDTEubiquitination[4]
357ASLEAAAKRKLSGLEubiquitination[1, 4]
359LEAAAKRKLSGLEQPubiquitination[1, 2, 4, 5]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [4] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [5] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
 Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
 J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572]
 [6] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 
Functional Description
  
Sequence Annotation
 REPEAT 40 80 WD 1.
 REPEAT 83 122 WD 2.
 REPEAT 128 168 WD 3.
 REPEAT 179 220 WD 4.
 REPEAT 224 266 WD 5.
 REPEAT 267 306 WD 6.
 MOD_RES 214 214 Phosphoserine.
 MOD_RES 361 361 Phosphoserine.  
Keyword
 Alternative splicing; Complete proteome; Nucleus; Phosphoprotein; Reference proteome; Repeat; WD repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 385 AA 
Protein Sequence
MAAAAARWNH VWVGTETGIL KGVNLQRKQA ANFTAGGQPR REEAVSALCW GTGGETQMLV 60
GCADRTVKHF STEDGIFQGQ RHCPGGEGMF RGLAQADGTL ITCVDSGILR VWHDKDKDTS 120
SDPLLELRVG PGVCRMRQDP AHPHVVATGG KENALKIWDL QGSEEPVFRA KNVRNDWLDL 180
RVPIWDQDIQ FLPGSQKLVT CTGYHQVRVY DPASPQRRPV LETTYGEYPL TAMTLTPGGN 240
SVIVGNTHGQ LAEIDLRQGR LLGCLKGLAG SVRGLQCHPS KPLLASCGLD RVLRIHRIQN 300
PRGLEHKVYL KSQLNCLLLS GRDNWEDEPQ EPQEPNKVPL EDTETDELWA SLEAAAKRKL 360
SGLEQPQGAL QTRRRKKKRP GSTSP 385 
Gene Ontology
 GO:0005730; C:nucleolus; IDA:UniProtKB.
 GO:0001825; P:blastocyst formation; IEA:Compara.
 GO:0016070; P:RNA metabolic process; IEA:Compara. 
Interpro
 IPR015943; WD40/YVTN_repeat-like_dom.
 IPR001680; WD40_repeat.
 IPR017986; WD40_repeat_dom. 
Pfam
 PF00400; WD40 
SMART
 SM00320; WD40 
PROSITE
 PS00678; WD_REPEATS_1
 PS50082; WD_REPEATS_2
 PS50294; WD_REPEATS_REGION 
PRINTS