CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-006036
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Ezrin 
Protein Synonyms/Alias
 Cytovillin; Villin-2; p81 
Gene Name
 Ezr 
Gene Synonyms/Alias
 Vil2 
Created Date
 July 27, 2013 
Organism
 Rattus norvegicus (Rat) 
NCBI Taxa ID
 10116 
Lysine Modification
Position
Peptide
Type
References
60KGFPTWLKLDKKVSAacetylation[1]
79KENPVQFKFRAKFYPacetylation[1]
139AKFGDYNKEMHKSGYacetylation[1]
143DYNKEMHKSGYLSSEacetylation[1]
162QRVMDQHKLSRDQWEacetylation[1]
184AEHRGMLKDSAMLEYacetylation[1]
337RETVEREKEQMLREKacetylation[1]
344KEQMLREKEELMLRLacetylation[1]
438LEEARRRKEDEVEEWacetylation[1]
458EAQDDLVKTKEELHLubiquitination[2]
523KRITEAEKNERVQRQacetylation[1]
Reference
 [1] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
 Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
 Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405]
 [2] Synaptic protein ubiquitination in rat brain revealed by antibody-based ubiquitome analysis.
 Na CH, Jones DR, Yang Y, Wang X, Xu Y, Peng J.
 J Proteome Res. 2012 Sep 7;11(9):4722-32. [PMID: 22871113] 
Functional Description
 Probably involved in connections of major cytoskeletal structures to the plasma membrane. In epithelial cells, required for the formation of microvilli and membrane ruffles on the apical pole. Along with PLEKHG6, required for normal macropinocytosis (By similarity). 
Sequence Annotation
 DOMAIN 2 295 FERM.
 REGION 244 586 Interaction with SCYL3 (By similarity).
 MOD_RES 60 60 N6-acetyllysine (By similarity).
 MOD_RES 146 146 Phosphotyrosine; by PDGFR (By
 MOD_RES 535 535 Phosphoserine (By similarity).
 MOD_RES 567 567 Phosphothreonine; by ROCK2 and PKC/PRKCI  
Keyword
 Acetylation; Cell membrane; Cell projection; Cell shape; Coiled coil; Complete proteome; Cytoplasm; Cytoskeleton; Direct protein sequencing; Membrane; Phosphoprotein; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 586 AA 
Protein Sequence
MPKPINVRVT TMDAELEFAI QPNTTGKQLF DQVVKTIGLR EVWYFGLQYV DNKGFPTWLK 60
LDKKVSAQEV RKENPVQFKF RAKFYPEDVA DELIQDITQK LFFLQVKEGI LSDEIYCPPE 120
TAVLLGSYAV QAKFGDYNKE MHKSGYLSSE RLIPQRVMDQ HKLSRDQWED RIQVWHAEHR 180
GMLKDSAMLE YLKIAQDLEM YGINYFEIKN KKGTDLWLGV DALGLNIYEK DDKLTPKIGF 240
PWSEIRNISF NDKKFVIKPI DKKAPDFVFY APRLRINKRI LQLCMGNHEL YMRRRKPDTI 300
EVQQMKAQAR EEKHQKQLER QQLETEKKRR ETVEREKEQM LREKEELMLR LQDFEQKTKR 360
AEKELSEQIE KALQLEEERR RAQEEAERLE ADRMAALRAK EELERQAQDQ IKSQEQLAAE 420
LAEYTAKIAL LEEARRRKED EVEEWQHRAK EAQDDLVKTK EELHLVMTAP PPPPPPVYEP 480
VNYHVQEGLQ DEGAEPMGYS AELSSEGILD DRNEEKRITE AEKNERVQRQ LLTLSNELSQ 540
ARDENKRTHN DIIHNENMRQ GRDKYKTLRQ IRQGNTKQRI DEFEAM 586 
Gene Ontology
 GO:0005884; C:actin filament; ISS:UniProtKB.
 GO:0016324; C:apical plasma membrane; IDA:UniProtKB.
 GO:0097449; C:astrocyte projection; IDA:RGD.
 GO:0016323; C:basolateral plasma membrane; IDA:UniProtKB.
 GO:0044297; C:cell body; IDA:RGD.
 GO:0005938; C:cell cortex; IEA:UniProtKB-SubCell.
 GO:0051286; C:cell tip; IDA:RGD.
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0019898; C:extrinsic to membrane; ISS:UniProtKB.
 GO:0030175; C:filopodium; IDA:RGD.
 GO:0005925; C:focal adhesion; IEA:Compara.
 GO:0045121; C:membrane raft; IDA:RGD.
 GO:0044393; C:microspike; IDA:RGD.
 GO:0005932; C:microtubule basal body; IEA:Compara.
 GO:0031528; C:microvillus membrane; IDA:UniProtKB.
 GO:0005730; C:nucleolus; IEA:Compara.
 GO:0032587; C:ruffle membrane; IEA:UniProtKB-SubCell.
 GO:0097454; C:Schwann cell microvillus; IDA:RGD.
 GO:0030315; C:T-tubule; IDA:RGD.
 GO:0001931; C:uropod; IEA:Compara.
 GO:0051015; F:actin filament binding; ISS:UniProtKB.
 GO:0050839; F:cell adhesion molecule binding; ISS:BHF-UCL.
 GO:0005198; F:structural molecule activity; TAS:RGD.
 GO:0051017; P:actin filament bundle assembly; ISS:UniProtKB.
 GO:0030855; P:epithelial cell differentiation; IEP:RGD.
 GO:0035088; P:establishment or maintenance of apical/basal cell polarity; IEA:Compara.
 GO:0046847; P:filopodium assembly; IMP:RGD.
 GO:0007159; P:leukocyte cell-cell adhesion; IEA:Compara.
 GO:0022614; P:membrane to membrane docking; IEA:Compara.
 GO:0008360; P:regulation of cell shape; IEA:UniProtKB-KW. 
Interpro
 IPR019749; Band_41_domain.
 IPR019750; Band_41_fam.
 IPR011174; ERM.
 IPR011259; ERM_C_dom.
 IPR000798; Ez/rad/moesin_like.
 IPR014352; FERM/acyl-CoA-bd_prot_3-hlx.
 IPR019748; FERM_central.
 IPR019747; FERM_CS.
 IPR000299; FERM_domain.
 IPR018979; FERM_N.
 IPR018980; FERM_PH-like_C.
 IPR008954; Moesin.
 IPR011993; PH_like_dom. 
Pfam
 PF00769; ERM
 PF09380; FERM_C
 PF00373; FERM_M
 PF09379; FERM_N 
SMART
 SM00295; B41 
PROSITE
 PS00660; FERM_1
 PS00661; FERM_2
 PS50057; FERM_3 
PRINTS
 PR00935; BAND41.
 PR00661; ERMFAMILY.