CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-002538
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 1,4-alpha-glucan branching enzyme GlgB 
Protein Synonyms/Alias
 1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase; Alpha-(1->4)-glucan branching enzyme; Glycogen branching enzyme; BE 
Gene Name
 glgB 
Gene Synonyms/Alias
 b3432; JW3395 
Created Date
 July 27, 2013 
Organism
 Escherichia coli (strain K12) 
NCBI Taxa ID
 83333 
Lysine Modification
Position
Peptide
Type
References
29FSVLGMHKTTAGLEVacetylation[1]
52DVWVIEPKTGRKLAKacetylation[1]
59KTGRKLAKLECLDSRacetylation[1]
170RHPMRLRKESGIWELacetylation[1]
674FGINQPGKWREILNTacetylation[1]
Reference
 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618] 
Functional Description
 Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position (By similarity). 
Sequence Annotation
 ACT_SITE 405 405 Nucleophile (By similarity).
 ACT_SITE 458 458 Proton donor (By similarity).  
Keyword
 3D-structure; Carbohydrate metabolism; Complete proteome; Glycogen biosynthesis; Glycogen metabolism; Glycosyltransferase; Reference proteome; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 728 AA 
Protein Sequence
MSDRIDRDVI NALIAGHFAD PFSVLGMHKT TAGLEVRALL PDATDVWVIE PKTGRKLAKL 60
ECLDSRGFFS GVIPRRKNFF RYQLAVVWHG QQNLIDDPYR FGPLIQEMDA WLLSEGTHLR 120
PYETLGAHAD TMDGVTGTRF SVWAPNARRV SVVGQFNYWD GRRHPMRLRK ESGIWELFIP 180
GAHNGQLYKY EMIDANGNLR LKSDPYAFEA QMRPETASLI CGLPEKVVQT EERKKANQFD 240
APISIYEVHL GSWRRHTDNN FWLSYRELAD QLVPYAKWMG FTHLELLPIN EHPFDGSWGY 300
QPTGLYAPTR RFGTRDDFRY FIDAAHAAGL NVILDWVPGH FPTDDFALAE FDGTNLYEHS 360
DPREGYHQDW NTLIYNYGRR EVSNFLVGNA LYWIERFGID ALRVDAVASM IYRDYSRKEG 420
EWIPNEFGGR ENLEAIEFLR NTNRILGEQV SGAVTMAEES TDFPGVSRPQ DMGGLGFWYK 480
WNLGWMHDTL DYMKLDPVYR QYHHDKLTFG ILYNYTENFV LPLSHDEVVH GKKSILDRMP 540
GDAWQKFANL RAYYGWMWAF PGKKLLFMGN EFAQGREWNH DASLDWHLLE GGDNWHHGVQ 600
RLVRDLNLTY RHHKAMHELD FDPYGFEWLV VDDKERSVLI FVRRDKEGNE IIVASNFTPV 660
PRHDYRFGIN QPGKWREILN TDSMHYHGSN AGNGGTVHSD EIASHGRQHS LSLTLPPLAT 720
IWLVREAE 728 
Gene Ontology
 GO:0003844; F:1,4-alpha-glucan branching enzyme activity; IDA:EcoCyc.
 GO:0043169; F:cation binding; IEA:InterPro.
 GO:0004553; F:hydrolase activity, hydrolyzing O-glycosyl compounds; IEA:InterPro.
 GO:0005978; P:glycogen biosynthetic process; IMP:EcoCyc.
 GO:0006974; P:response to DNA damage stimulus; IEP:EcoliWiki. 
Interpro
 IPR006407; 1-4-A-glucan_branch_enz.
 IPR006048; A-amylase_b_C.
 IPR015902; Glyco_hydro_13.
 IPR013780; Glyco_hydro_13_b.
 IPR006047; Glyco_hydro_13_cat_dom.
 IPR004193; Glyco_hydro_13_N.
 IPR013781; Glyco_hydro_catalytic_dom.
 IPR017853; Glycoside_hydrolase_SF.
 IPR013783; Ig-like_fold.
 IPR014756; Ig_E-set. 
Pfam
 PF00128; Alpha-amylase
 PF02806; Alpha-amylase_C
 PF02922; CBM_48 
SMART
  
PROSITE
  
PRINTS