CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-002104
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Ornithine aminotransferase, mitochondrial 
Protein Synonyms/Alias
 Ornithine delta-aminotransferase; Ornithine--oxo-acid aminotransferase; Ornithine aminotransferase, hepatic form; Ornithine aminotransferase, renal form 
Gene Name
 OAT 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
31SATSVATKKTVQGPPubiquitination[1]
32ATSVATKKTVQGPPTubiquitination[1, 2, 3, 4]
49DIFEREYKYGAHNYHacetylation[5]
49DIFEREYKYGAHNYHubiquitination[1, 2, 4]
66PVALERGKGIYLWDVubiquitination[1, 2, 3, 4]
102PKIVNALKSQVDKLTubiquitination[1]
107ALKSQVDKLTLTSRAubiquitination[1]
351NLAENADKLGIILRNubiquitination[1]
362ILRNELMKLPSDVVTacetylation[5]
362ILRNELMKLPSDVVTubiquitination[1, 3, 6]
374VVTAVRGKGLLNAIVubiquitination[1, 2, 3, 4]
383LLNAIVIKETKDWDAubiquitination[1]
386AIVIKETKDWDAWKVacetylation[5]
392TKDWDAWKVCLRLRDubiquitination[2, 4]
405RDNGLLAKPTHGDIIacetylation[5]
405RDNGLLAKPTHGDIIubiquitination[1, 3, 6]
421FAPPLVIKEDELRESubiquitination[1]
Reference
 [1] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [4] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [5] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [6] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961
Functional Description
  
Sequence Annotation
 MOD_RES 292 292 N6-(pyridoxal phosphate)lysine.  
Keyword
 3D-structure; Alternative splicing; Aminotransferase; Complete proteome; Direct protein sequencing; Disease mutation; Mitochondrion; Polymorphism; Pyridoxal phosphate; Reference proteome; Transferase; Transit peptide. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 439 AA 
Protein Sequence
MFSKLAHLQR FAVLSRGVHS SVASATSVAT KKTVQGPPTS DDIFEREYKY GAHNYHPLPV 60
ALERGKGIYL WDVEGRKYFD FLSSYSAVNQ GHCHPKIVNA LKSQVDKLTL TSRAFYNNVL 120
GEYEEYITKL FNYHKVLPMN TGVEAGETAC KLARKWGYTV KGIQKYKAKI VFAAGNFWGR 180
TLSAISSSTD PTSYDGFGPF MPGFDIIPYN DLPALERALQ DPNVAAFMVE PIQGEAGVVV 240
PDPGYLMGVR ELCTRHQVLF IADEIQTGLA RTGRWLAVDY ENVRPDIVLL GKALSGGLYP 300
VSAVLCDDDI MLTIKPGEHG STYGGNPLGC RVAIAALEVL EEENLAENAD KLGIILRNEL 360
MKLPSDVVTA VRGKGLLNAI VIKETKDWDA WKVCLRLRDN GLLAKPTHGD IIRFAPPLVI 420
KEDELRESIE IINKTILSF 439 
Gene Ontology
 GO:0005759; C:mitochondrial matrix; TAS:Reactome.
 GO:0004587; F:ornithine-oxo-acid transaminase activity; EXP:Reactome.
 GO:0030170; F:pyridoxal phosphate binding; IEA:InterPro.
 GO:0008652; P:cellular amino acid biosynthetic process; TAS:Reactome.
 GO:0034641; P:cellular nitrogen compound metabolic process; TAS:Reactome.
 GO:0055129; P:L-proline biosynthetic process; IEA:UniProtKB-UniPathway.
 GO:0034214; P:protein hexamerization; IDA:UniProtKB.
 GO:0007601; P:visual perception; TAS:ProtInc. 
Interpro
 IPR005814; Aminotrans_3.
 IPR010164; Orn_aminotrans.
 IPR015424; PyrdxlP-dep_Trfase.
 IPR015421; PyrdxlP-dep_Trfase_major_sub1.
 IPR015422; PyrdxlP-dep_Trfase_major_sub2. 
Pfam
 PF00202; Aminotran_3 
SMART
  
PROSITE
 PS00600; AA_TRANSFER_CLASS_3 
PRINTS