CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-020806
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Putative tRNA pseudouridine synthase Pus10 
Protein Synonyms/Alias
 Coiled-coil domain-containing protein 139; tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase 
Gene Name
 Pus10 
Gene Synonyms/Alias
 Ccdc139 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
249FTRMAVLKALSKIKEacetylation[1]
434IGFLDDLKDLKIDQKubiquitination[2]
485TQAGTYIKEFVHGDFubiquitination[2]
Reference
 [1] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
 [2] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 
Functional Description
 Pseudouridylate synthases catalyze pseudouridination of structural RNAs, including transfer, ribosomal, and splicing RNAs. PUS10 catalyzes the formation of the universal psi55 in the GC loop of transfer RNAs (Probable). Modulator of TRAIL-induced cell death via activation of procaspase 8 and BID cleavage. Required for the progression of the apoptotic signal through intrinsic mitochondrial cell death (By similarity). 
Sequence Annotation
 REGION 302 315 RNA binding forefinger loop (Potential).
 REGION 440 455 RNA binding thumb loop (Potential).
 ACT_SITE 342 342 Nucleophile (Potential).
 BINDING 412 412 Substrate (Potential).
 BINDING 483 483 Substrate (Potential).
 MOD_RES 82 82 Phosphoserine.  
Keyword
 Coiled coil; Complete proteome; Isomerase; Phosphoprotein; Reference proteome; tRNA processing. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 527 AA 
Protein Sequence
MLPLTEENKH VAQLLFSSGT CPRCILRFCG VDLPAPYKHP SKELLNELQK FLEPEKPELI 60
LEAPNPPLKK IRLHEDGIDN LSEDGKEGVS VTEDESMAEK PSKLRVCNVC LGILQEFCEK 120
GFITKVCQKV EASGFEFTSV VLSVSFPPQL SVREHAAWLL VKQEMGKQSL SLGRNDVVQL 180
KEAYKWITHP LFSEELGVPT DGKSLFEVSV VFAHPETAED CHFLGEVCRD CFKPAKNKQS 240
VFTRMAVLKA LSKIKEEDFL GQFPCPPNSP KTVCTVLEVE CTHGAVFVAG RYNKYSRNLP 300
QTPWIIDGER KMESSVEELI SDHLLAVFRA ESFNFSSSGR EDVDVRTLGN GRPFAVELLN 360
PHRVHFTSQE MKELQQTINK SSDKIQVRDL QLVTREAIGH MKEGEEEKTK TYSALIWTNR 420
AIQKKDIGFL DDLKDLKIDQ KTPLRVLHRR PLAVRTRAIH SMKTHYLDEH HFRLHLKTQA 480
GTYIKEFVHG DFGRTKPNLG SLMNVTADIL ELDVESVDVD WPPALDD 527 
Gene Ontology
 GO:0009982; F:pseudouridine synthase activity; IEA:InterPro.
 GO:0003723; F:RNA binding; IEA:InterPro.
 GO:0001522; P:pseudouridine synthesis; IEA:InterPro.
 GO:0008033; P:tRNA processing; IEA:UniProtKB-KW. 
Interpro
 IPR020103; PsdUridine_synth_cat_dom. 
Pfam
  
SMART
  
PROSITE
  
PRINTS