CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-018319
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Butyrophilin subfamily 2 member A2 
Protein Synonyms/Alias
  
Gene Name
 BTN2A2 
Gene Synonyms/Alias
 BT2.2; BTF2 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
61RCHLSPEKNAEDMEVubiquitination[1]
83SPAVFVYKGGRERTEubiquitination[1]
105GRITFVSKDINRGSVubiquitination[1, 2]
152VVAGLGSKPLIEIKAubiquitination[1, 2]
307KKVEQEEKEIAQQLQubiquitination[1, 2]
406CRHSVERKGEVLLIPubiquitination[2]
440PERILPLKESLCRVGubiquitination[1]
513MVPEEGLKLHRVGTHubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983] 
Functional Description
 Inhibits the proliferation of CD4 and CD8 T-cells activated by anti-CD3 antibodies, T-cell metabolism and IL2 and IFNG secretion (By similarity). 
Sequence Annotation
 DOMAIN 34 145 Ig-like V-type.
 DOMAIN 153 234 Ig-like C2-type.
 DOMAIN 309 502 B30.2/SPRY.
 CARBOHYD 50 50 N-linked (GlcNAc...) (Potential).
 CARBOHYD 118 118 N-linked (GlcNAc...) (Potential).
 CARBOHYD 220 220 N-linked (GlcNAc...) (Potential).
 CARBOHYD 226 226 N-linked (GlcNAc...) (Potential).
 DISULFID 55 129 By similarity.  
Keyword
 Alternative splicing; Coiled coil; Complete proteome; Disulfide bond; Glycoprotein; Immunoglobulin domain; Membrane; Polymorphism; Reference proteome; Signal; Transmembrane; Transmembrane helix. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 523 AA 
Protein Sequence
MEPAAALHFS LPASLLLLLL LLLLSLCALV SAQFTVVGPA NPILAMVGEN TTLRCHLSPE 60
KNAEDMEVRW FRSQFSPAVF VYKGGRERTE EQMEEYRGRI TFVSKDINRG SVALVIHNVT 120
AQENGIYRCY FQEGRSYDEA ILRLVVAGLG SKPLIEIKAQ EDGSIWLECI SGGWYPEPLT 180
VWRDPYGEVV PALKEVSIAD ADGLFMVTTA VIIRDKYVRN VSCSVNNTLL GQEKETVIFI 240
PESFMPSASP WMVALAVILT ASPWMVSMTV ILAVFIIFMA VSICCIKKLQ REKKILSGEK 300
KVEQEEKEIA QQLQEELRWR RTFLHAADVV LDPDTAHPEL FLSEDRRSVR RGPYRQRVPD 360
NPERFDSQPC VLGWESFASG KHYWEVEVEN VMVWTVGVCR HSVERKGEVL LIPQNGFWTL 420
EMFGNQYRAL SSPERILPLK ESLCRVGVFL DYEAGDVSFY NMRDRSHIYT CPRSAFTVPV 480
RPFFRLGSDD SPIFICPALT GASGVMVPEE GLKLHRVGTH QSL 523 
Gene Ontology
 GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
 GO:0046007; P:negative regulation of activated T cell proliferation; ISS:UniProtKB.
 GO:0031324; P:negative regulation of cellular metabolic process; ISS:UniProtKB.
 GO:0050710; P:negative regulation of cytokine secretion; ISS:UniProtKB. 
Interpro
 IPR001870; B30.2/SPRY.
 IPR003879; Butyrophylin.
 IPR013162; CD80_C2-set.
 IPR008985; ConA-like_lec_gl_sf.
 IPR007110; Ig-like_dom.
 IPR013783; Ig-like_fold.
 IPR003599; Ig_sub.
 IPR013106; Ig_V-set.
 IPR006574; PRY.
 IPR018355; SPla/RYanodine_receptor_subgr.
 IPR003877; SPRY_rcpt. 
Pfam
 PF08205; C2-set_2
 PF00622; SPRY
 PF07686; V-set 
SMART
 SM00409; IG
 SM00589; PRY
 SM00449; SPRY 
PROSITE
 PS50188; B302_SPRY
 PS50835; IG_LIKE 
PRINTS
 PR01407; BUTYPHLNCDUF.