UniProt Accession

 PSPF_ECOLI; P37344; P76039 

Genbank Protein ID

 U38542; U00096; AP009048; X57560 

Genbank Nucleotide ID

 AAB02186.1; AAC74385.2; BAA14872.1 

Protein Name

 Psp operon transcriptional activator 

Protein Synonyms/Alias

 Phage shock protein F 

Gene Name

 pspF 

Gene Synonyms/Alias

 ycjB; b1303; JW1296 

Created Date

 July 27, 2013 

Organism

 Escherichia coli (strain K12) 

NCBI Taxa ID

 83333 

Lysine Modification

Position	Peptide	Type	References
202	IQMCREIKLPLFPGF	acetylation	[1]
256	DIIIDPFKRRPPEDA	acetylation	[1]
299	QLSLQQGKYNQKRAA	acetylation	[1]

Reference

 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618] 

Functional Description

 Transcriptional activator for the phage shock protein (psp) operon (pspABCDE) and pspG gene. 

Sequence Annotation

 DOMAIN 15 237 Sigma-54 factor interaction.
 NP_BIND 36 43 ATP (Potential).
 NP_BIND 99 108 ATP (Potential).
 DNA_BIND 302 321 H-T-H motif (By similarity).  

Keyword

 3D-structure; Activator; ATP-binding; Complete proteome; Cytoplasm; DNA-binding; Nucleotide-binding; Reference proteome; Transcription; Transcription regulation; Two-component regulatory system. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 325 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
 GO:0017111; F:nucleoside-triphosphatase activity; IEA:InterPro.
 GO:0003700; F:sequence-specific DNA binding transcription factor activity; IEA:InterPro.
 GO:0045892; P:negative regulation of transcription, DNA-dependent; ISS:EcoCyc.
 GO:0000160; P:phosphorelay signal transduction system; IEA:UniProtKB-KW.
 GO:0045893; P:positive regulation of transcription, DNA-dependent; ISS:EcoCyc.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 

Interpro

 IPR003593; AAA+_ATPase.
 IPR009057; Homeodomain-like.
 IPR002197; HTH_Fis.
 IPR027417; P-loop_NTPase.
 IPR002078; Sigma_54_int.
 IPR025943; Sigma_54_int_dom_ATP-bd_2.
 IPR025944; Sigma_54_int_dom_CS.
 IPR014317; Transcription_activator_PspF. 

Pfam

 PF02954; HTH_8
 PF00158; Sigma54_activat 

SMART

 SM00382; AAA 

PROSITE

 PS00675; SIGMA54_INTERACT_1
 PS00676; SIGMA54_INTERACT_2
 PS00688; SIGMA54_INTERACT_3
 PS50045; SIGMA54_INTERACT_4 

PRINTS

 PR01590; HTHFIS.