UniProt Accession

 RAC3_HUMAN; P60763; O14658; Q5U0M8 

Genbank Protein ID

 AF008591; AF498966; BT019443; BC009605; BC015197 

Genbank Nucleotide ID

 AAC51667.1; AAM21113.1; AAV38250.1; AAH09605.1; AAH15197.1 

Protein Name

 Ras-related C3 botulinum toxin substrate 3 

Protein Synonyms/Alias

 p21-Rac3 

Gene Name

 RAC3 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
96	SFENVRAKWYPEVRH	ubiquitination	[1]
133	IERLRDKKLAPITYP	ubiquitination	[1]
153	AREIGSVKYLECSAL	ubiquitination	[1, 2]
183	VLCPPPVKKPGKKCT	ubiquitination	[2, 3]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] Mass spectrometric analysis of lysine ubiquitylation reveals promiscuity at site level.
 Danielsen JM, Sylvestersen KB, Bekker-Jensen S, Szklarczyk D, Poulsen JW, Horn H, Jensen LJ, Mailand N, Nielsen ML.
 Mol Cell Proteomics. 2011 Mar;10(3):M110.003590. [PMID: 21139048] 

Functional Description

 Plasma membrane-associated small GTPase which cycles between an active GTP-bound and inactive GDP-bound state. In active state binds to a variety of effector proteins to regulate cellular responses, such as cell spreading and the formation of actin-based protusions including lamellipodia and membrane ruffles. 

Sequence Annotation

 NP_BIND 10 17 GTP (By similarity).
 NP_BIND 57 61 GTP (By similarity).
 NP_BIND 115 118 GTP (By similarity).
 MOTIF 32 40 Effector region (Potential).
 MOD_RES 189 189 Cysteine methyl ester (By similarity).
 LIPID 189 189 S-geranylgeranyl cysteine (By  

Keyword

 3D-structure; Cell projection; Complete proteome; Cytoplasm; GTP-binding; Lipoprotein; Membrane; Methylation; Nucleotide-binding; Prenylation; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 192 AA 

Protein Sequence

Gene Ontology

 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0012505; C:endomembrane system; IDA:UniProtKB.
 GO:0031941; C:filamentous actin; IDA:MGI.
 GO:0030426; C:growth cone; IDA:MGI.
 GO:0030027; C:lamellipodium; IEA:UniProtKB-SubCell.
 GO:0043025; C:neuronal cell body; IDA:MGI.
 GO:0005886; C:plasma membrane; TAS:Reactome.
 GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
 GO:0003924; F:GTPase activity; IDA:UniProtKB.
 GO:0030036; P:actin cytoskeleton organization; IDA:UniProtKB.
 GO:0030031; P:cell projection assembly; IDA:UniProtKB.
 GO:0050885; P:neuromuscular process controlling balance; IEA:Compara.
 GO:0031175; P:neuron projection development; IEA:Compara.
 GO:0051056; P:regulation of small GTPase mediated signal transduction; TAS:Reactome.
 GO:0007264; P:small GTPase mediated signal transduction; TAS:Reactome. 

Interpro

 IPR027417; P-loop_NTPase.
 IPR005225; Small_GTP-bd_dom.
 IPR001806; Small_GTPase.
 IPR003578; Small_GTPase_Rho. 

Pfam

 PF00071; Ras 

SMART

 SM00174; RHO 

PROSITE

 PS51420; RHO 

PRINTS

 PR00449; RASTRNSFRMNG.