UniProt Accession

 CBF5_YEAST; P33322; D6VYH9 

Genbank Protein ID

 L12351; U17246; BK006945 

Genbank Nucleotide ID

 AAA34473.1; AAB67463.1; DAA09495.1 

Protein Name

 H/ACA ribonucleoprotein complex subunit 4 

Protein Synonyms/Alias

 Centromere-binding factor 5; Centromere/microtubule-binding protein CBF5; H/ACA snoRNP protein CBF5; Small nucleolar RNP protein CBF5; p64' 

Gene Name

 CBF5 

Gene Synonyms/Alias

 YLR175W; L9470.11 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
3	*****MSKEDFVIKP	acetylation	[1]
9	SKEDFVIKPEAAGAS	acetylation	[1]
9	SKEDFVIKPEAAGAS	ubiquitination	[2, 3]
31	LLLKNFDKLLVRSGH	acetylation	[1]
31	LLLKNFDKLLVRSGH	ubiquitination	[3]
50	PAGSSPLKRDLKSYI	ubiquitination	[3]
80	HEVVAWIKRILRCEK	ubiquitination	[3]
87	KRILRCEKTGHSGTL	ubiquitination	[3]
114	DRATRLVKSQQGAGK	ubiquitination	[3]
121	KSQQGAGKEYVCIVR	ubiquitination	[3]
134	VRLHDALKDEKDLGR	acetylation	[1]
137	HDALKDEKDLGRSLE	ubiquitination	[2]
161	PPLISAVKRQLRVRT	ubiquitination	[3]
180	NLIEFDNKRNLGVFW	acetylation	[1]
180	NLIEFDNKRNLGVFW	ubiquitination	[3]
267	ETLLVGYKRIVVKDS	ubiquitination	[2, 3]
272	GYKRIVVKDSAVNAV	ubiquitination	[3]
384	NTPEQWKKEYVPLDN	ubiquitination	[3]

Reference

 [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]
 [2] Sites of ubiquitin attachment in Saccharomyces cerevisiae.
 Starita LM, Lo RS, Eng JK, von Haller PD, Fields S.
 Proteomics. 2012 Jan;12(2):236-40. [PMID: 22106047]
 [3] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301] 

Functional Description

 Plays a central role in ribosomal RNA processing. Probable catalytic subunit of H/ACA small nucleolar ribonucleoprotein (H/ACA snoRNP) complex, which catalyzes pseudouridylation of rRNA. This involves the isomerization of uridine such that the ribose is subsequently attached to C5, instead of the normal N1. Pseudouridine ('psi') residues may serve to stabilize the conformation of rRNAs. May function as a pseudouridine synthase. Binds in vitro to centromeres and microtubules. It is a centromeric DNA-CBF3-binding factor which is involved in mitotic chromosome segregation. Essential for cell growth. 

Sequence Annotation

 DOMAIN 266 341 PUA.
 REPEAT 434 436 1.
 REPEAT 437 439 2.
 REPEAT 440 442 3.
 REPEAT 443 445 4.
 REPEAT 446 448 5.
 REPEAT 449 451 6.
 REPEAT 452 454 7.
 REPEAT 455 457 8.
 REPEAT 458 460 9.
 REPEAT 461 463 10.
 REGION 434 463 10 X 3 AA tandem repeats of K-K-[DE].
 ACT_SITE 95 95 Nucleophile (By similarity).
 MOD_RES 47 47 Phosphoserine.
 MOD_RES 378 378 Phosphothreonine.
 MOD_RES 399 399 Phosphoserine; by ATM or ATR.  

Keyword

 3D-structure; Cell cycle; Cell division; Centromere; Chromosome; Complete proteome; Cytoplasm; Cytoskeleton; Direct protein sequencing; DNA-binding; Isomerase; Microtubule; Mitosis; Nucleus; Phosphoprotein; Reference proteome; Repeat; Ribonucleoprotein; Ribosome biogenesis; RNA-binding; rRNA processing. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 483 AA 

Protein Sequence

Gene Ontology

 GO:0030686; C:90S preribosome; IDA:SGD.
 GO:0031429; C:box H/ACA snoRNP complex; IPI:SGD.
 GO:0000775; C:chromosome, centromeric region; IEA:UniProtKB-SubCell.
 GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
 GO:0005874; C:microtubule; IEA:UniProtKB-KW.
 GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
 GO:0009982; F:pseudouridine synthase activity; IMP:SGD.
 GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
 GO:0000495; P:box H/ACA snoRNA 3'-end processing; IMP:SGD.
 GO:0051301; P:cell division; IEA:UniProtKB-KW.
 GO:0007067; P:mitosis; IEA:UniProtKB-KW.
 GO:0031118; P:rRNA pseudouridine synthesis; IMP:SGD.
 GO:0031120; P:snRNA pseudouridine synthesis; IDA:SGD. 

Interpro

 IPR012960; Dyskerin-like.
 IPR002501; PsdUridine_synth.
 IPR020103; PsdUridine_synth_cat_dom.
 IPR002478; PUA.
 IPR015947; PUA-like_domain.
 IPR004802; tRNA_PsdUridine_synth_B_fam.
 IPR004521; Uncharacterised_CHP00451. 

Pfam

 PF08068; DKCLD
 PF01472; PUA
 PF01509; TruB_N 

SMART

 SM00359; PUA 

PROSITE

 PS50890; PUA 

PRINTS