CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-023254
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Probable dimethyladenosine transferase 
Protein Synonyms/Alias
 DIM1 dimethyladenosine transferase 1 homolog; DIM1 dimethyladenosine transferase 1-like; Probable 18S rRNA (adenine(1779)-N(6)/adenine(1780)-N(6))-dimethyltransferase; Probable 18S rRNA dimethylase; Probable S-adenosylmethionine-6-N',N'-adenosyl(rRNA) dimethyltransferase 
Gene Name
 DIMT1 
Gene Synonyms/Alias
 DIMT1L; HUSSY-05 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
96RLVAELHKRVQGTPVubiquitination[1]
106QGTPVASKLQVLVGDubiquitination[2, 3, 4]
253AVQQLLEKNYRIHCSubiquitination[1, 2, 3, 4]
288TSTGFSDKRARSMDIubiquitination[1, 3]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [4] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 
Functional Description
 Specifically dimethylates two adjacent adenosines in the loop of a conserved hairpin near the 3'-end of 18S rRNA in the 40S particle (By similarity). 
Sequence Annotation
 BINDING 37 37 S-adenosyl-L-methionine; via carbonyl
 BINDING 39 39 S-adenosyl-L-methionine; via amide
 BINDING 64 64 S-adenosyl-L-methionine; via carbonyl
 BINDING 85 85 S-adenosyl-L-methionine.
 BINDING 113 113 S-adenosyl-L-methionine.
 BINDING 128 128 S-adenosyl-L-methionine.  
Keyword
 3D-structure; Complete proteome; Methyltransferase; Nucleus; Reference proteome; RNA-binding; rRNA processing; S-adenosyl-L-methionine; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 313 AA 
Protein Sequence
MPKVKSGAIG RRRGRQEQRR ELKSAGGLMF NTGIGQHILK NPLIINSIID KAALRPTDVV 60
LEVGPGTGNM TVKLLEKAKK VVACELDPRL VAELHKRVQG TPVASKLQVL VGDVLKTDLP 120
FFDTCVANLP YQISSPFVFK LLLHRPFFRC AILMFQREFA LRLVAKPGDK LYCRLSINTQ 180
LLARVDHLMK VGKNNFRPPP KVESSVVRIE PKNPPPPINF QEWDGLVRIT FVRKNKTLSA 240
AFKSSAVQQL LEKNYRIHCS VHNIIIPEDF SIADKIQQIL TSTGFSDKRA RSMDIDDFIR 300
LLHGFNAEGI HFS 313 
Gene Ontology
 GO:0005730; C:nucleolus; IEA:UniProtKB-SubCell.
 GO:0052909; F:18S rRNA (adenine(1779)-N(6)/adenine(1780)-N(6))-dimethyltransferase activity; IEA:EC.
 GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
 GO:0000179; F:rRNA (adenine-N6,N6-)-dimethyltransferase activity; IEA:InterPro.
 GO:0031167; P:rRNA methylation; IEA:GOC. 
Interpro
 IPR025814; DIM1.
 IPR020596; rRNA_Ade_Mease_Trfase_CS.
 IPR001737; rRNA_Ade_methylase_transferase.
 IPR020598; rRNA_Ade_methylase_Trfase_N.
 IPR011530; rRNA_adenine_dimethylase. 
Pfam
 PF00398; RrnaAD 
SMART
 SM00650; rADc 
PROSITE
 PS01131; RRNA_A_DIMETH 
PRINTS